SIMPLIFIED DIAGNOSIS OF MALARIA INFECTION: GFM/PCR/ELISA A SIMPLIFIED NUCLEIC ACID AMPLIFICATION TECHNIQUE BY PCR/ELISA

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Ricardo Luiz Dantas MACHADO, Denise Oliveira GARRET, Ipemida Sullayman ADAGU, David Charles WARHURST, Marinete Marins PÓVOA
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 1998
• Subjects: Malaria; Diagnosis; Glass fibre membrane; PCR/ELISA; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doi.org/10.1590/S0036-46651998000500014; https://doaj.org/article/1260e4aaba7a4a899768ac277658f0c9

We report an adaptation of a technique for the blood sample collection (GFM) as well as for the extraction and amplification of Plasmodium DNA for the diagnosis of malaria infection by the PCR/ELISA. The method of blood sample collection requires less expertise and saves both time and money, thus reducing the cost by more than half. The material is also suitable for genetic analysis in either fresh or stored specimens prepared by this method. Relatamos a adaptação de uma técnica para coleta de amostras (MFV) e outra para extração, amplificação de DNA de parasitas da malária para diagnóstico por PCR/ELISA. O método de coleta de amostras requer menos habilidade e economisa tempo e dinheiro, assim reduzindo a mais da metade o custo. O material é também adequado para análise genética em especimens frescos ou estocados, preparados por este método.