Gametogenesis in the Pacific oyster Crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes.
BACKGROUND: The Pacific oyster Crassostrea gigas (Mollusca, Lophotrochozoa) is an alternative and irregular protandrous hermaphrodite: most individuals mature first as males and then change sex several times. Little is known about genetic and phenotypic basis of sex differentiation in oysters, and l...
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ftdoajarticles:oai:doaj.org/article:124feb13552a4ec8b2c882be05dd64b7 2023-05-15T15:58:18+02:00 Gametogenesis in the Pacific oyster Crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes. Nolwenn M Dheilly Christophe Lelong Arnaud Huvet Kristell Kellner Marie-Pierre Dubos Guillaume Riviere Pierre Boudry Pascal Favrel 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0036353 https://doaj.org/article/124feb13552a4ec8b2c882be05dd64b7 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3348941?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0036353 https://doaj.org/article/124feb13552a4ec8b2c882be05dd64b7 PLoS ONE, Vol 7, Iss 5, p e36353 (2012) Medicine R Science Q article 2012 ftdoajarticles https://doi.org/10.1371/journal.pone.0036353 2022-12-31T05:33:35Z BACKGROUND: The Pacific oyster Crassostrea gigas (Mollusca, Lophotrochozoa) is an alternative and irregular protandrous hermaphrodite: most individuals mature first as males and then change sex several times. Little is known about genetic and phenotypic basis of sex differentiation in oysters, and little more about the molecular pathways regulating reproduction. We have recently developed and validated a microarray containing 31,918 oligomers (Dheilly et al., 2011) representing the oyster transcriptome. The application of this microarray to the study of mollusk gametogenesis should provide a better understanding of the key factors involved in sex differentiation and the regulation of oyster reproduction. METHODOLOGY/PRINCIPAL FINDINGS: Gene expression was studied in gonads of oysters cultured over a yearly reproductive cycle. Principal component analysis and hierarchical clustering showed a significant divergence in gene expression patterns of males and females coinciding with the start of gonial mitosis. ANOVA analysis of the data revealed 2,482 genes differentially expressed during the course of males and/or females gametogenesis. The expression of 434 genes could be localized in either germ cells or somatic cells of the gonad by comparing the transcriptome of female gonads to the transcriptome of stripped oocytes and somatic tissues. Analysis of the annotated genes revealed conserved molecular mechanisms between mollusks and mammals: genes involved in chromatin condensation, DNA replication and repair, mitosis and meiosis regulation, transcription, translation and apoptosis were expressed in both male and female gonads. Most interestingly, early expressed male-specific genes included bindin and a dpy-30 homolog and female-specific genes included foxL2, nanos homolog 3, a pancreatic lipase related protein, cd63 and vitellogenin. Further functional analyses are now required in order to investigate their role in sex differentiation in oysters. CONCLUSIONS/SIGNIFICANCE: This study allowed us to identify potential ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific PLoS ONE 7 5 e36353 |
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Medicine R Science Q |
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Medicine R Science Q Nolwenn M Dheilly Christophe Lelong Arnaud Huvet Kristell Kellner Marie-Pierre Dubos Guillaume Riviere Pierre Boudry Pascal Favrel Gametogenesis in the Pacific oyster Crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes. |
topic_facet |
Medicine R Science Q |
description |
BACKGROUND: The Pacific oyster Crassostrea gigas (Mollusca, Lophotrochozoa) is an alternative and irregular protandrous hermaphrodite: most individuals mature first as males and then change sex several times. Little is known about genetic and phenotypic basis of sex differentiation in oysters, and little more about the molecular pathways regulating reproduction. We have recently developed and validated a microarray containing 31,918 oligomers (Dheilly et al., 2011) representing the oyster transcriptome. The application of this microarray to the study of mollusk gametogenesis should provide a better understanding of the key factors involved in sex differentiation and the regulation of oyster reproduction. METHODOLOGY/PRINCIPAL FINDINGS: Gene expression was studied in gonads of oysters cultured over a yearly reproductive cycle. Principal component analysis and hierarchical clustering showed a significant divergence in gene expression patterns of males and females coinciding with the start of gonial mitosis. ANOVA analysis of the data revealed 2,482 genes differentially expressed during the course of males and/or females gametogenesis. The expression of 434 genes could be localized in either germ cells or somatic cells of the gonad by comparing the transcriptome of female gonads to the transcriptome of stripped oocytes and somatic tissues. Analysis of the annotated genes revealed conserved molecular mechanisms between mollusks and mammals: genes involved in chromatin condensation, DNA replication and repair, mitosis and meiosis regulation, transcription, translation and apoptosis were expressed in both male and female gonads. Most interestingly, early expressed male-specific genes included bindin and a dpy-30 homolog and female-specific genes included foxL2, nanos homolog 3, a pancreatic lipase related protein, cd63 and vitellogenin. Further functional analyses are now required in order to investigate their role in sex differentiation in oysters. CONCLUSIONS/SIGNIFICANCE: This study allowed us to identify potential ... |
format |
Article in Journal/Newspaper |
author |
Nolwenn M Dheilly Christophe Lelong Arnaud Huvet Kristell Kellner Marie-Pierre Dubos Guillaume Riviere Pierre Boudry Pascal Favrel |
author_facet |
Nolwenn M Dheilly Christophe Lelong Arnaud Huvet Kristell Kellner Marie-Pierre Dubos Guillaume Riviere Pierre Boudry Pascal Favrel |
author_sort |
Nolwenn M Dheilly |
title |
Gametogenesis in the Pacific oyster Crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes. |
title_short |
Gametogenesis in the Pacific oyster Crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes. |
title_full |
Gametogenesis in the Pacific oyster Crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes. |
title_fullStr |
Gametogenesis in the Pacific oyster Crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes. |
title_full_unstemmed |
Gametogenesis in the Pacific oyster Crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes. |
title_sort |
gametogenesis in the pacific oyster crassostrea gigas: a microarrays-based analysis identifies sex and stage specific genes. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pone.0036353 https://doaj.org/article/124feb13552a4ec8b2c882be05dd64b7 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
PLoS ONE, Vol 7, Iss 5, p e36353 (2012) |
op_relation |
http://europepmc.org/articles/PMC3348941?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0036353 https://doaj.org/article/124feb13552a4ec8b2c882be05dd64b7 |
op_doi |
https://doi.org/10.1371/journal.pone.0036353 |
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PLoS ONE |
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7 |
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5 |
container_start_page |
e36353 |
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