Multiple Anopheles species complicate downstream analysis and decision-making in a malaria pre-elimination area in southern Mozambique
Abstract Background Different anopheline species (even within a species group/complex) can differ in their feeding and resting behaviours, which impact both malaria transmission patterns as well as the efficacy of vector control interventions. While morphological identification of sampled specimens...
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ftdoajarticles:oai:doaj.org/article:12495982eda14a74826c3754e860ff94 2024-02-27T08:38:24+00:00 Multiple Anopheles species complicate downstream analysis and decision-making in a malaria pre-elimination area in southern Mozambique Mara Máquina Mercy A. Opiyo Nelson Cuamba Dulcisária Marrenjo Maria Rodrigues Sarmento Armando Sheila Nhate Fabião Luis Francisco Saúte Baltazar Candrinho Neil F. Lobo Krijn P. Paaijmans 2024-01-01T00:00:00Z https://doi.org/10.1186/s12936-024-04842-0 https://doaj.org/article/12495982eda14a74826c3754e860ff94 EN eng BMC https://doi.org/10.1186/s12936-024-04842-0 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-024-04842-0 1475-2875 https://doaj.org/article/12495982eda14a74826c3754e860ff94 Malaria Journal, Vol 23, Iss 1, Pp 1-6 (2024) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2024 ftdoajarticles https://doi.org/10.1186/s12936-024-04842-0 2024-01-28T02:25:28Z Abstract Background Different anopheline species (even within a species group/complex) can differ in their feeding and resting behaviours, which impact both malaria transmission patterns as well as the efficacy of vector control interventions. While morphological identification of sampled specimens is an important first step towards understanding species diversity and abundance, misidentification can result in the implementation of less effective vector control measures, and consequently smaller reductions in the number of local malaria cases. Focusing on southern Mozambique, a malaria pre-elimination area where malaria remains persistent, the aims of this preliminary study were to use molecular identification (CO1 and ITS2 barcoding) to (1) validate the results from the morphological identification (with a particular focus on Anopheles pharoensis and Anopheles squamosus), and (2) have a closer look at the Anopheles coustani group (which includes Anopheles tenebrosus and Anopheles ziemanni). Methods Female anopheline mosquitoes (n = 81) were identified morphologically and subsequently sequenced at the ribosomal DNA internal transcribed spacer region 2 (ITS2) and/or cytochrome oxidase subunit 1 (CO1) loci towards species determination. Results Out of the 62 specimens that were identified morphologically to species, 4 (6.5%) were misidentified. Regarding the An. coustani group, morphological identification showed that several members are present in southern Mozambique, including An. coustani sensu lato (s.l.), An. ziemanni and An. tenebrosus. However, based on both ITS2 and CO1 sequences, the exact species remains unknown for the latter two members until voucher sequences are available for comparison. Conclusion The reason(s) for morphological misidentification of anopheline mosquitoes need to be mitigated. This is usually related to both the capacity (i.e. training) of the microscopist to identify anopheline species, and the information provided in the dichotomous identification key. As the An. coustani complex ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 23 1 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Mara Máquina Mercy A. Opiyo Nelson Cuamba Dulcisária Marrenjo Maria Rodrigues Sarmento Armando Sheila Nhate Fabião Luis Francisco Saúte Baltazar Candrinho Neil F. Lobo Krijn P. Paaijmans Multiple Anopheles species complicate downstream analysis and decision-making in a malaria pre-elimination area in southern Mozambique |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Different anopheline species (even within a species group/complex) can differ in their feeding and resting behaviours, which impact both malaria transmission patterns as well as the efficacy of vector control interventions. While morphological identification of sampled specimens is an important first step towards understanding species diversity and abundance, misidentification can result in the implementation of less effective vector control measures, and consequently smaller reductions in the number of local malaria cases. Focusing on southern Mozambique, a malaria pre-elimination area where malaria remains persistent, the aims of this preliminary study were to use molecular identification (CO1 and ITS2 barcoding) to (1) validate the results from the morphological identification (with a particular focus on Anopheles pharoensis and Anopheles squamosus), and (2) have a closer look at the Anopheles coustani group (which includes Anopheles tenebrosus and Anopheles ziemanni). Methods Female anopheline mosquitoes (n = 81) were identified morphologically and subsequently sequenced at the ribosomal DNA internal transcribed spacer region 2 (ITS2) and/or cytochrome oxidase subunit 1 (CO1) loci towards species determination. Results Out of the 62 specimens that were identified morphologically to species, 4 (6.5%) were misidentified. Regarding the An. coustani group, morphological identification showed that several members are present in southern Mozambique, including An. coustani sensu lato (s.l.), An. ziemanni and An. tenebrosus. However, based on both ITS2 and CO1 sequences, the exact species remains unknown for the latter two members until voucher sequences are available for comparison. Conclusion The reason(s) for morphological misidentification of anopheline mosquitoes need to be mitigated. This is usually related to both the capacity (i.e. training) of the microscopist to identify anopheline species, and the information provided in the dichotomous identification key. As the An. coustani complex ... |
format |
Article in Journal/Newspaper |
author |
Mara Máquina Mercy A. Opiyo Nelson Cuamba Dulcisária Marrenjo Maria Rodrigues Sarmento Armando Sheila Nhate Fabião Luis Francisco Saúte Baltazar Candrinho Neil F. Lobo Krijn P. Paaijmans |
author_facet |
Mara Máquina Mercy A. Opiyo Nelson Cuamba Dulcisária Marrenjo Maria Rodrigues Sarmento Armando Sheila Nhate Fabião Luis Francisco Saúte Baltazar Candrinho Neil F. Lobo Krijn P. Paaijmans |
author_sort |
Mara Máquina |
title |
Multiple Anopheles species complicate downstream analysis and decision-making in a malaria pre-elimination area in southern Mozambique |
title_short |
Multiple Anopheles species complicate downstream analysis and decision-making in a malaria pre-elimination area in southern Mozambique |
title_full |
Multiple Anopheles species complicate downstream analysis and decision-making in a malaria pre-elimination area in southern Mozambique |
title_fullStr |
Multiple Anopheles species complicate downstream analysis and decision-making in a malaria pre-elimination area in southern Mozambique |
title_full_unstemmed |
Multiple Anopheles species complicate downstream analysis and decision-making in a malaria pre-elimination area in southern Mozambique |
title_sort |
multiple anopheles species complicate downstream analysis and decision-making in a malaria pre-elimination area in southern mozambique |
publisher |
BMC |
publishDate |
2024 |
url |
https://doi.org/10.1186/s12936-024-04842-0 https://doaj.org/article/12495982eda14a74826c3754e860ff94 |
geographic |
Arctic |
geographic_facet |
Arctic |
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Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 23, Iss 1, Pp 1-6 (2024) |
op_relation |
https://doi.org/10.1186/s12936-024-04842-0 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-024-04842-0 1475-2875 https://doaj.org/article/12495982eda14a74826c3754e860ff94 |
op_doi |
https://doi.org/10.1186/s12936-024-04842-0 |
container_title |
Malaria Journal |
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23 |
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1 |
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1792045291072913408 |