Minimally Symptomatic Infection in an Ebola 'Hotspot': A Cross-Sectional Serosurvey.
INTRODUCTION:Evidence for minimally symptomatic Ebola virus (EBOV) infection is limited. During the 2013-16 outbreak in West Africa, it was not considered epidemiologically relevant to published models or projections of intervention effects. In order to improve our understanding of the transmission...
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ftdoajarticles:oai:doaj.org/article:122e674174424500b99232bce6549573 2023-05-15T15:14:50+02:00 Minimally Symptomatic Infection in an Ebola 'Hotspot': A Cross-Sectional Serosurvey. Eugene T Richardson J Daniel Kelly Mohamed Bailor Barrie Annelies W Mesman Sahr Karku Komba Quiwa Regan H Marsh Songor Koedoyoma Fodei Daboh Kathryn P Barron Michael Grady Elizabeth Tucker Kerry L Dierberg George W Rutherford Michele Barry James Holland Jones Megan B Murray Paul E Farmer 2016-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005087 https://doaj.org/article/122e674174424500b99232bce6549573 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5112953?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005087 https://doaj.org/article/122e674174424500b99232bce6549573 PLoS Neglected Tropical Diseases, Vol 10, Iss 11, p e0005087 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005087 2022-12-31T06:28:13Z INTRODUCTION:Evidence for minimally symptomatic Ebola virus (EBOV) infection is limited. During the 2013-16 outbreak in West Africa, it was not considered epidemiologically relevant to published models or projections of intervention effects. In order to improve our understanding of the transmission dynamics of EBOV in humans, we investigated the occurrence of minimally symptomatic EBOV infection in quarantined contacts of reported Ebola virus disease cases in a recognized 'hotspot.' METHODOLOGY/PRINCIPAL FINDINGS:We conducted a cross-sectional serosurvey in Sukudu, Kono District, Sierra Leone, from October 2015 to January 2016. A blood sample was collected from 187 study participants, 132 negative controls (individuals with a low likelihood of previous exposure to Ebola virus), and 30 positive controls (Ebola virus disease survivors). IgG responses to Ebola glycoprotein and nucleoprotein were measured using Alpha Diagnostic International ELISA kits with plasma diluted at 1:200. Optical density was read at 450 nm (subtracting OD at 630nm to normalize well background) on a ChroMate 4300 microplate reader. A cutoff of 4.7 U/mL for the anti-GP ELISA yielded 96.7% sensitivity and 97.7% specificity in distinguishing positive and negative controls. We identified 14 seropositive individuals not known to have had Ebola virus disease. Two of the 14 seropositive individuals reported only fever during quarantine while the remaining 12 denied any signs or symptoms during quarantine. CONCLUSIONS/SIGNIFICANCE:By using ELISA to measure Zaire Ebola virus antibody concentrations, we identified a significant number of individuals with previously undetected EBOV infection in a 'hotspot' village in Sierra Leone, approximately one year after the village outbreak. The findings provide further evidence that Ebola, like many other viral infections, presents with a spectrum of clinical manifestations, including minimally symptomatic infection. These data also suggest that a significant portion of Ebola transmission events may have gone ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 11 e0005087 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Eugene T Richardson J Daniel Kelly Mohamed Bailor Barrie Annelies W Mesman Sahr Karku Komba Quiwa Regan H Marsh Songor Koedoyoma Fodei Daboh Kathryn P Barron Michael Grady Elizabeth Tucker Kerry L Dierberg George W Rutherford Michele Barry James Holland Jones Megan B Murray Paul E Farmer Minimally Symptomatic Infection in an Ebola 'Hotspot': A Cross-Sectional Serosurvey. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
INTRODUCTION:Evidence for minimally symptomatic Ebola virus (EBOV) infection is limited. During the 2013-16 outbreak in West Africa, it was not considered epidemiologically relevant to published models or projections of intervention effects. In order to improve our understanding of the transmission dynamics of EBOV in humans, we investigated the occurrence of minimally symptomatic EBOV infection in quarantined contacts of reported Ebola virus disease cases in a recognized 'hotspot.' METHODOLOGY/PRINCIPAL FINDINGS:We conducted a cross-sectional serosurvey in Sukudu, Kono District, Sierra Leone, from October 2015 to January 2016. A blood sample was collected from 187 study participants, 132 negative controls (individuals with a low likelihood of previous exposure to Ebola virus), and 30 positive controls (Ebola virus disease survivors). IgG responses to Ebola glycoprotein and nucleoprotein were measured using Alpha Diagnostic International ELISA kits with plasma diluted at 1:200. Optical density was read at 450 nm (subtracting OD at 630nm to normalize well background) on a ChroMate 4300 microplate reader. A cutoff of 4.7 U/mL for the anti-GP ELISA yielded 96.7% sensitivity and 97.7% specificity in distinguishing positive and negative controls. We identified 14 seropositive individuals not known to have had Ebola virus disease. Two of the 14 seropositive individuals reported only fever during quarantine while the remaining 12 denied any signs or symptoms during quarantine. CONCLUSIONS/SIGNIFICANCE:By using ELISA to measure Zaire Ebola virus antibody concentrations, we identified a significant number of individuals with previously undetected EBOV infection in a 'hotspot' village in Sierra Leone, approximately one year after the village outbreak. The findings provide further evidence that Ebola, like many other viral infections, presents with a spectrum of clinical manifestations, including minimally symptomatic infection. These data also suggest that a significant portion of Ebola transmission events may have gone ... |
format |
Article in Journal/Newspaper |
author |
Eugene T Richardson J Daniel Kelly Mohamed Bailor Barrie Annelies W Mesman Sahr Karku Komba Quiwa Regan H Marsh Songor Koedoyoma Fodei Daboh Kathryn P Barron Michael Grady Elizabeth Tucker Kerry L Dierberg George W Rutherford Michele Barry James Holland Jones Megan B Murray Paul E Farmer |
author_facet |
Eugene T Richardson J Daniel Kelly Mohamed Bailor Barrie Annelies W Mesman Sahr Karku Komba Quiwa Regan H Marsh Songor Koedoyoma Fodei Daboh Kathryn P Barron Michael Grady Elizabeth Tucker Kerry L Dierberg George W Rutherford Michele Barry James Holland Jones Megan B Murray Paul E Farmer |
author_sort |
Eugene T Richardson |
title |
Minimally Symptomatic Infection in an Ebola 'Hotspot': A Cross-Sectional Serosurvey. |
title_short |
Minimally Symptomatic Infection in an Ebola 'Hotspot': A Cross-Sectional Serosurvey. |
title_full |
Minimally Symptomatic Infection in an Ebola 'Hotspot': A Cross-Sectional Serosurvey. |
title_fullStr |
Minimally Symptomatic Infection in an Ebola 'Hotspot': A Cross-Sectional Serosurvey. |
title_full_unstemmed |
Minimally Symptomatic Infection in an Ebola 'Hotspot': A Cross-Sectional Serosurvey. |
title_sort |
minimally symptomatic infection in an ebola 'hotspot': a cross-sectional serosurvey. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0005087 https://doaj.org/article/122e674174424500b99232bce6549573 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 11, p e0005087 (2016) |
op_relation |
http://europepmc.org/articles/PMC5112953?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005087 https://doaj.org/article/122e674174424500b99232bce6549573 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005087 |
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PLOS Neglected Tropical Diseases |
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11 |
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e0005087 |
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