Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from Deschampsia antarctica and its expression regulated by cold and UV stresses
Abstract Background The Copper/Zinc superoxide dismutase (Cu/ZnSOD) gene, SOD gene, was isolated from a Deschampsia antarctica Desv. by cDNA library screening. The expression of SOD gene in the leaves of D. antarctica was determined by RT-PCR and its differential expression of gene transcripts in co...
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ftdoajarticles:oai:doaj.org/article:122c31d6d0c54a1a8b6c38fae016acfb 2023-05-15T13:44:39+02:00 Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from Deschampsia antarctica and its expression regulated by cold and UV stresses Gidekel Manuel Moraga Ana Dinamarca Jorge Sánchez-Venegas Jaime R 2009-09-01T00:00:00Z https://doi.org/10.1186/1756-0500-2-198 https://doaj.org/article/122c31d6d0c54a1a8b6c38fae016acfb EN eng BMC http://www.biomedcentral.com/1756-0500/2/198 https://doaj.org/toc/1756-0500 doi:10.1186/1756-0500-2-198 1756-0500 https://doaj.org/article/122c31d6d0c54a1a8b6c38fae016acfb BMC Research Notes, Vol 2, Iss 1, p 198 (2009) Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 article 2009 ftdoajarticles https://doi.org/10.1186/1756-0500-2-198 2022-12-31T06:53:54Z Abstract Background The Copper/Zinc superoxide dismutase (Cu/ZnSOD) gene, SOD gene, was isolated from a Deschampsia antarctica Desv. by cDNA library screening. The expression of SOD gene in the leaves of D. antarctica was determined by RT-PCR and its differential expression of gene transcripts in conditions of cold and UV radiation stresses was revealed by northern blot. Findings The molecular characterization shows that SOD cDNA is 709 bp in length, which translates an ORF of 152 amino acids that correspond to a protein of predicted molecular mass of 15 kDa. The assay shows that the expression of SOD gene increases when D. antarctica is acclimatised to 4°C and exposed to UV radiation. These results indicate that the SOD gene of D. antarctica is involved in the antioxidative process triggered by oxidative stress induced by the conditions of environmental change in which they live. Conclusion The present results allow us to know the characteristics of Cu/ZnSOD gene from D. antarctica and understand that its expression is regulated by cold and UV radiation. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles BMC Research Notes 2 1 198 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 |
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Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 Gidekel Manuel Moraga Ana Dinamarca Jorge Sánchez-Venegas Jaime R Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from Deschampsia antarctica and its expression regulated by cold and UV stresses |
topic_facet |
Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 |
description |
Abstract Background The Copper/Zinc superoxide dismutase (Cu/ZnSOD) gene, SOD gene, was isolated from a Deschampsia antarctica Desv. by cDNA library screening. The expression of SOD gene in the leaves of D. antarctica was determined by RT-PCR and its differential expression of gene transcripts in conditions of cold and UV radiation stresses was revealed by northern blot. Findings The molecular characterization shows that SOD cDNA is 709 bp in length, which translates an ORF of 152 amino acids that correspond to a protein of predicted molecular mass of 15 kDa. The assay shows that the expression of SOD gene increases when D. antarctica is acclimatised to 4°C and exposed to UV radiation. These results indicate that the SOD gene of D. antarctica is involved in the antioxidative process triggered by oxidative stress induced by the conditions of environmental change in which they live. Conclusion The present results allow us to know the characteristics of Cu/ZnSOD gene from D. antarctica and understand that its expression is regulated by cold and UV radiation. |
format |
Article in Journal/Newspaper |
author |
Gidekel Manuel Moraga Ana Dinamarca Jorge Sánchez-Venegas Jaime R |
author_facet |
Gidekel Manuel Moraga Ana Dinamarca Jorge Sánchez-Venegas Jaime R |
author_sort |
Gidekel Manuel |
title |
Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from Deschampsia antarctica and its expression regulated by cold and UV stresses |
title_short |
Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from Deschampsia antarctica and its expression regulated by cold and UV stresses |
title_full |
Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from Deschampsia antarctica and its expression regulated by cold and UV stresses |
title_fullStr |
Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from Deschampsia antarctica and its expression regulated by cold and UV stresses |
title_full_unstemmed |
Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from Deschampsia antarctica and its expression regulated by cold and UV stresses |
title_sort |
molecular characterization of a cdna encoding cu/zn superoxide dismutase from deschampsia antarctica and its expression regulated by cold and uv stresses |
publisher |
BMC |
publishDate |
2009 |
url |
https://doi.org/10.1186/1756-0500-2-198 https://doaj.org/article/122c31d6d0c54a1a8b6c38fae016acfb |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
BMC Research Notes, Vol 2, Iss 1, p 198 (2009) |
op_relation |
http://www.biomedcentral.com/1756-0500/2/198 https://doaj.org/toc/1756-0500 doi:10.1186/1756-0500-2-198 1756-0500 https://doaj.org/article/122c31d6d0c54a1a8b6c38fae016acfb |
op_doi |
https://doi.org/10.1186/1756-0500-2-198 |
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BMC Research Notes |
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2 |
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1 |
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198 |
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1766204653997916160 |