Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages
RNA viruses have short generation times and high mutation rates, allowing them to undergo rapid molecular evolution during epidemics. However, the extent of RNA virus phenotypic evolution within epidemics and the resulting effects on fitness and virulence remain mostly unknown. Here, we screened the...
Main Authors: | , , , , , , , , , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2023
|
Subjects: | |
Online Access: | https://doaj.org/article/12277ab9345d479ebd7beb6966e2c4de |
id |
ftdoajarticles:oai:doaj.org/article:12277ab9345d479ebd7beb6966e2c4de |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:12277ab9345d479ebd7beb6966e2c4de 2023-05-15T15:16:30+02:00 Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages Glenn Oliveira Chantal B. F. Vogels Ashley Zolfaghari Sharada Saraf Raphaelle Klitting James Weger-Lucarelli Karla P. Leon Carlos O. Ontiveros Rimjhim Agarwal Konstantin A. Tsetsarkin Eva Harris Gregory D. Ebel Shirlee Wohl Nathan D. Grubaugh Kristian G. Andersen 2023-02-01T00:00:00Z https://doaj.org/article/12277ab9345d479ebd7beb6966e2c4de EN eng Public Library of Science (PLoS) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9907835/?tool=EBI https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 https://doaj.org/article/12277ab9345d479ebd7beb6966e2c4de PLoS Neglected Tropical Diseases, Vol 17, Iss 2 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles 2023-02-12T01:27:53Z RNA viruses have short generation times and high mutation rates, allowing them to undergo rapid molecular evolution during epidemics. However, the extent of RNA virus phenotypic evolution within epidemics and the resulting effects on fitness and virulence remain mostly unknown. Here, we screened the 2015–2016 Zika epidemic in the Americas for lineage-specific fitness differences. We engineered a library of recombinant viruses representing twelve major Zika virus lineages and used them to measure replicative fitness within disease-relevant human primary cells and live mosquitoes. We found that two of these lineages conferred significant in vitro replicative fitness changes among human primary cells, but we did not find fitness changes in Aedes aegypti mosquitoes. Additionally, we found evidence for elevated levels of positive selection among five amino acid sites that define major Zika virus lineages. While our work suggests that Zika virus may have acquired several phenotypic changes during a short time scale, these changes were relatively moderate and do not appear to have enhanced transmission during the epidemic. Author summary Zika virus was introduced to the Western Hemisphere, spread rapidly, and led to the 2015–2016 Zika epidemic and a rise in congenital microcephaly. It remains unclear whether Zika virus evolved to become more transmissible directly before or during the epidemic. To investigate whether Zika evolved to become more transmissible, we engineered a library of recombinant viruses that represent twelve major Zika virus lineages that circulated throughout the Americas. We measured the replicative fitness of each of these lineages by infecting live mosquitoes and human cells that are relevant for disease or transmission. We found that two of the lineages, one that dominated Central America and another that existed mostly in the Caribbean, appear to replicate more efficiently in human cells. While the fitness changes do not appear to have significant effects on the 2015–2016 Zika epidemic, our ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Glenn Oliveira Chantal B. F. Vogels Ashley Zolfaghari Sharada Saraf Raphaelle Klitting James Weger-Lucarelli Karla P. Leon Carlos O. Ontiveros Rimjhim Agarwal Konstantin A. Tsetsarkin Eva Harris Gregory D. Ebel Shirlee Wohl Nathan D. Grubaugh Kristian G. Andersen Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
RNA viruses have short generation times and high mutation rates, allowing them to undergo rapid molecular evolution during epidemics. However, the extent of RNA virus phenotypic evolution within epidemics and the resulting effects on fitness and virulence remain mostly unknown. Here, we screened the 2015–2016 Zika epidemic in the Americas for lineage-specific fitness differences. We engineered a library of recombinant viruses representing twelve major Zika virus lineages and used them to measure replicative fitness within disease-relevant human primary cells and live mosquitoes. We found that two of these lineages conferred significant in vitro replicative fitness changes among human primary cells, but we did not find fitness changes in Aedes aegypti mosquitoes. Additionally, we found evidence for elevated levels of positive selection among five amino acid sites that define major Zika virus lineages. While our work suggests that Zika virus may have acquired several phenotypic changes during a short time scale, these changes were relatively moderate and do not appear to have enhanced transmission during the epidemic. Author summary Zika virus was introduced to the Western Hemisphere, spread rapidly, and led to the 2015–2016 Zika epidemic and a rise in congenital microcephaly. It remains unclear whether Zika virus evolved to become more transmissible directly before or during the epidemic. To investigate whether Zika evolved to become more transmissible, we engineered a library of recombinant viruses that represent twelve major Zika virus lineages that circulated throughout the Americas. We measured the replicative fitness of each of these lineages by infecting live mosquitoes and human cells that are relevant for disease or transmission. We found that two of the lineages, one that dominated Central America and another that existed mostly in the Caribbean, appear to replicate more efficiently in human cells. While the fitness changes do not appear to have significant effects on the 2015–2016 Zika epidemic, our ... |
format |
Article in Journal/Newspaper |
author |
Glenn Oliveira Chantal B. F. Vogels Ashley Zolfaghari Sharada Saraf Raphaelle Klitting James Weger-Lucarelli Karla P. Leon Carlos O. Ontiveros Rimjhim Agarwal Konstantin A. Tsetsarkin Eva Harris Gregory D. Ebel Shirlee Wohl Nathan D. Grubaugh Kristian G. Andersen |
author_facet |
Glenn Oliveira Chantal B. F. Vogels Ashley Zolfaghari Sharada Saraf Raphaelle Klitting James Weger-Lucarelli Karla P. Leon Carlos O. Ontiveros Rimjhim Agarwal Konstantin A. Tsetsarkin Eva Harris Gregory D. Ebel Shirlee Wohl Nathan D. Grubaugh Kristian G. Andersen |
author_sort |
Glenn Oliveira |
title |
Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages |
title_short |
Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages |
title_full |
Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages |
title_fullStr |
Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages |
title_full_unstemmed |
Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages |
title_sort |
genomic and phenotypic analyses suggest moderate fitness differences among zika virus lineages |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doaj.org/article/12277ab9345d479ebd7beb6966e2c4de |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 2 (2023) |
op_relation |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9907835/?tool=EBI https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 https://doaj.org/article/12277ab9345d479ebd7beb6966e2c4de |
_version_ |
1766346802893684736 |