Performance of molecular methods for identification of unusual subtypes of hepatitis C virus genotype 2
Introduction: Hepatitis C virus (HCV) displays high genetic variability, with seven genotypes and numerous subtypes. The determination of the viral type has been essential for the selection and timing of antiviral treatment. In Venezuela, HCV genotype 2 is relatively diverse, being particularly prev...
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ftdoajarticles:oai:doaj.org/article:11b8188ed6d7466fb1ac80caacf79255 2023-05-15T15:07:00+02:00 Performance of molecular methods for identification of unusual subtypes of hepatitis C virus genotype 2 Pierina D’Angelo Rossana Celeste Jaspe Carmen Luisa Loureiro Cristina Gutiérrez María Zulay Sulbarán Yoneira Sulbarán Felix Toro Flor Helene Pujol 2018-06-01T00:00:00Z https://doi.org/10.7705/biomedica.v38i0.3864 https://doaj.org/article/11b8188ed6d7466fb1ac80caacf79255 EN ES eng spa Instituto Nacional de Salud https://www.revistabiomedica.org/index.php/biomedica/article/view/3864 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v38i0.3864 https://doaj.org/article/11b8188ed6d7466fb1ac80caacf79255 Biomédica: revista del Instituto Nacional de Salud, Vol 38, Iss 2, Pp 282-288 (2018) Medicine R Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.7705/biomedica.v38i0.3864 2022-12-31T02:05:15Z Introduction: Hepatitis C virus (HCV) displays high genetic variability, with seven genotypes and numerous subtypes. The determination of the viral type has been essential for the selection and timing of antiviral treatment. In Venezuela, HCV genotype 2 is relatively diverse, being particularly prevalent subtype 2j. Objective: To evaluate the performance of methodologies for genotyping HCV, particularly for identification of subtype 2j. Materials and methods: HCV genotype and subtype were determined by reverse hybridization technique (LiPA) and sequencing of the HCV 5’UTR and NS5B regions. Results: A total of 65 samples from HCV-infected patients were analyzed. PCR amplifications of the 5’UTR region exhibited the highest sensitivity (100% vs 91% for LiPA and 77% for NS5B). Genotype determination, taking as reference test NS5B, showed 100% concordance with the other methods, and 67% and 59% for subtypes with 5´NC and LiPA, respectively. NS5B sequencing allowed the identification of subtypes 2j and 2s, which were not detected by the other methods. A specific LiPA pattern was not observed for HCV subtype 2j. Conclusion: Although being the methodology with lowest sensitivity for amplification of HCV RNA, sequencing NS5B region remains a powerful tool for correct discrimination of the different HCV subtypes, which is of epidemiological relevance. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 38 2 282 288 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English Spanish |
topic |
Medicine R Arctic medicine. Tropical medicine RC955-962 |
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Medicine R Arctic medicine. Tropical medicine RC955-962 Pierina D’Angelo Rossana Celeste Jaspe Carmen Luisa Loureiro Cristina Gutiérrez María Zulay Sulbarán Yoneira Sulbarán Felix Toro Flor Helene Pujol Performance of molecular methods for identification of unusual subtypes of hepatitis C virus genotype 2 |
topic_facet |
Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: Hepatitis C virus (HCV) displays high genetic variability, with seven genotypes and numerous subtypes. The determination of the viral type has been essential for the selection and timing of antiviral treatment. In Venezuela, HCV genotype 2 is relatively diverse, being particularly prevalent subtype 2j. Objective: To evaluate the performance of methodologies for genotyping HCV, particularly for identification of subtype 2j. Materials and methods: HCV genotype and subtype were determined by reverse hybridization technique (LiPA) and sequencing of the HCV 5’UTR and NS5B regions. Results: A total of 65 samples from HCV-infected patients were analyzed. PCR amplifications of the 5’UTR region exhibited the highest sensitivity (100% vs 91% for LiPA and 77% for NS5B). Genotype determination, taking as reference test NS5B, showed 100% concordance with the other methods, and 67% and 59% for subtypes with 5´NC and LiPA, respectively. NS5B sequencing allowed the identification of subtypes 2j and 2s, which were not detected by the other methods. A specific LiPA pattern was not observed for HCV subtype 2j. Conclusion: Although being the methodology with lowest sensitivity for amplification of HCV RNA, sequencing NS5B region remains a powerful tool for correct discrimination of the different HCV subtypes, which is of epidemiological relevance. |
format |
Article in Journal/Newspaper |
author |
Pierina D’Angelo Rossana Celeste Jaspe Carmen Luisa Loureiro Cristina Gutiérrez María Zulay Sulbarán Yoneira Sulbarán Felix Toro Flor Helene Pujol |
author_facet |
Pierina D’Angelo Rossana Celeste Jaspe Carmen Luisa Loureiro Cristina Gutiérrez María Zulay Sulbarán Yoneira Sulbarán Felix Toro Flor Helene Pujol |
author_sort |
Pierina D’Angelo |
title |
Performance of molecular methods for identification of unusual subtypes of hepatitis C virus genotype 2 |
title_short |
Performance of molecular methods for identification of unusual subtypes of hepatitis C virus genotype 2 |
title_full |
Performance of molecular methods for identification of unusual subtypes of hepatitis C virus genotype 2 |
title_fullStr |
Performance of molecular methods for identification of unusual subtypes of hepatitis C virus genotype 2 |
title_full_unstemmed |
Performance of molecular methods for identification of unusual subtypes of hepatitis C virus genotype 2 |
title_sort |
performance of molecular methods for identification of unusual subtypes of hepatitis c virus genotype 2 |
publisher |
Instituto Nacional de Salud |
publishDate |
2018 |
url |
https://doi.org/10.7705/biomedica.v38i0.3864 https://doaj.org/article/11b8188ed6d7466fb1ac80caacf79255 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 38, Iss 2, Pp 282-288 (2018) |
op_relation |
https://www.revistabiomedica.org/index.php/biomedica/article/view/3864 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v38i0.3864 https://doaj.org/article/11b8188ed6d7466fb1ac80caacf79255 |
op_doi |
https://doi.org/10.7705/biomedica.v38i0.3864 |
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Biomédica |
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38 |
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2 |
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282 |
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288 |
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