PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples PCR-RFLP do 16S DNA ribossomal para confirmar a identificação de Enterococcus gallinarum e Enterococcus casseliflavus isolados de amostras clínicas e alimentares
INTRODUCTION: This study aimed to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples by PCR-RFLP. METHODS: Fifty-two strains identified by conventional biochemical exams were submitted to PCR amplification and digested with Hi...
Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
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Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2010
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Online Access: | https://doi.org/10.1590/S0037-86822010000100023 https://doaj.org/article/118b77cd54824130b16dc7b01ec04d56 |
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ftdoajarticles:oai:doaj.org/article:118b77cd54824130b16dc7b01ec04d56 2023-05-15T15:13:27+02:00 PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples PCR-RFLP do 16S DNA ribossomal para confirmar a identificação de Enterococcus gallinarum e Enterococcus casseliflavus isolados de amostras clínicas e alimentares Aline Weber Medeiros Pedro d'Azevedo Rebeca Inhoque Pereira Ana Paula Cassenego Sueli Van Der Sand Jeverson Frazzon Ana Paula Guedes Frazzon 2010-02-01T00:00:00Z https://doi.org/10.1590/S0037-86822010000100023 https://doaj.org/article/118b77cd54824130b16dc7b01ec04d56 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822010000100023 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 doi:10.1590/S0037-86822010000100023 0037-8682 1678-9849 https://doaj.org/article/118b77cd54824130b16dc7b01ec04d56 Revista da Sociedade Brasileira de Medicina Tropical, Vol 43, Iss 1, Pp 100-101 (2010) PCR-RFLP de 16S rDNA Enterococcus gallinarum Enterococcus casseliflavus PCR-RFLP of 16S rDNA Arctic medicine. Tropical medicine RC955-962 article 2010 ftdoajarticles https://doi.org/10.1590/S0037-86822010000100023 2022-12-30T23:08:11Z INTRODUCTION: This study aimed to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples by PCR-RFLP. METHODS: Fifty-two strains identified by conventional biochemical exams were submitted to PCR amplification and digested with HinfI. Only 20 (38.5%) of the 52 strains showed a DNA pattern expected for E. gallinarum and E. casseliflavus. RESULTS: Analysis of the results of this study showed that E. gallinarum and E. casseliflavus are occasionally erroneously identified and confirmed the potential application of 16S rDNA analysis for accurate identification of these species. CONCLUSIONS: A correct identification is important to distinguish between intrinsic and acquired vancomycin resistance. INTRODUÇÃO: O objetivo deste estudo foi confirmar a identificação de amostras clínicas e alimentos de Enterococcus gallinarum e Enterococcus casseliflavus por PCR-RFLP. MÉTODOS: Cinquenta e duas cepas identificadas por exames bioquímicos convencionais foram submetidos a amplificação por PCR e digestão com HinfI. Apenas 20 (38,5%) das 52 amostras apresentaram um padrão de DNA esperado E. gallinarum e E. casseliflavus. RESULTADOS: Analise dos resultados deste estudo demonstraram que, algumas vezes E. gallinarum e E. casseliflavus são erroneamente identificados e confirmaram a potencial aplicação da análise do 16S rDNA para identificação exata destas espécies. CONCLUSÕES: A correta identificação é importante a fim de distinguir entre resistência intrínseca e adquirida à vancomicina. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 43 1 100 101 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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PCR-RFLP de 16S rDNA Enterococcus gallinarum Enterococcus casseliflavus PCR-RFLP of 16S rDNA Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
PCR-RFLP de 16S rDNA Enterococcus gallinarum Enterococcus casseliflavus PCR-RFLP of 16S rDNA Arctic medicine. Tropical medicine RC955-962 Aline Weber Medeiros Pedro d'Azevedo Rebeca Inhoque Pereira Ana Paula Cassenego Sueli Van Der Sand Jeverson Frazzon Ana Paula Guedes Frazzon PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples PCR-RFLP do 16S DNA ribossomal para confirmar a identificação de Enterococcus gallinarum e Enterococcus casseliflavus isolados de amostras clínicas e alimentares |
topic_facet |
PCR-RFLP de 16S rDNA Enterococcus gallinarum Enterococcus casseliflavus PCR-RFLP of 16S rDNA Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: This study aimed to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples by PCR-RFLP. METHODS: Fifty-two strains identified by conventional biochemical exams were submitted to PCR amplification and digested with HinfI. Only 20 (38.5%) of the 52 strains showed a DNA pattern expected for E. gallinarum and E. casseliflavus. RESULTS: Analysis of the results of this study showed that E. gallinarum and E. casseliflavus are occasionally erroneously identified and confirmed the potential application of 16S rDNA analysis for accurate identification of these species. CONCLUSIONS: A correct identification is important to distinguish between intrinsic and acquired vancomycin resistance. INTRODUÇÃO: O objetivo deste estudo foi confirmar a identificação de amostras clínicas e alimentos de Enterococcus gallinarum e Enterococcus casseliflavus por PCR-RFLP. MÉTODOS: Cinquenta e duas cepas identificadas por exames bioquímicos convencionais foram submetidos a amplificação por PCR e digestão com HinfI. Apenas 20 (38,5%) das 52 amostras apresentaram um padrão de DNA esperado E. gallinarum e E. casseliflavus. RESULTADOS: Analise dos resultados deste estudo demonstraram que, algumas vezes E. gallinarum e E. casseliflavus são erroneamente identificados e confirmaram a potencial aplicação da análise do 16S rDNA para identificação exata destas espécies. CONCLUSÕES: A correta identificação é importante a fim de distinguir entre resistência intrínseca e adquirida à vancomicina. |
format |
Article in Journal/Newspaper |
author |
Aline Weber Medeiros Pedro d'Azevedo Rebeca Inhoque Pereira Ana Paula Cassenego Sueli Van Der Sand Jeverson Frazzon Ana Paula Guedes Frazzon |
author_facet |
Aline Weber Medeiros Pedro d'Azevedo Rebeca Inhoque Pereira Ana Paula Cassenego Sueli Van Der Sand Jeverson Frazzon Ana Paula Guedes Frazzon |
author_sort |
Aline Weber Medeiros |
title |
PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples PCR-RFLP do 16S DNA ribossomal para confirmar a identificação de Enterococcus gallinarum e Enterococcus casseliflavus isolados de amostras clínicas e alimentares |
title_short |
PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples PCR-RFLP do 16S DNA ribossomal para confirmar a identificação de Enterococcus gallinarum e Enterococcus casseliflavus isolados de amostras clínicas e alimentares |
title_full |
PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples PCR-RFLP do 16S DNA ribossomal para confirmar a identificação de Enterococcus gallinarum e Enterococcus casseliflavus isolados de amostras clínicas e alimentares |
title_fullStr |
PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples PCR-RFLP do 16S DNA ribossomal para confirmar a identificação de Enterococcus gallinarum e Enterococcus casseliflavus isolados de amostras clínicas e alimentares |
title_full_unstemmed |
PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples PCR-RFLP do 16S DNA ribossomal para confirmar a identificação de Enterococcus gallinarum e Enterococcus casseliflavus isolados de amostras clínicas e alimentares |
title_sort |
pcr-rflp of 16s ribosomal dna to confirm the identification of enterococcus gallinarum and enterococcus casseliflavus isolated from clinical and food samples pcr-rflp do 16s dna ribossomal para confirmar a identificação de enterococcus gallinarum e enterococcus casseliflavus isolados de amostras clínicas e alimentares |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2010 |
url |
https://doi.org/10.1590/S0037-86822010000100023 https://doaj.org/article/118b77cd54824130b16dc7b01ec04d56 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 43, Iss 1, Pp 100-101 (2010) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822010000100023 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 doi:10.1590/S0037-86822010000100023 0037-8682 1678-9849 https://doaj.org/article/118b77cd54824130b16dc7b01ec04d56 |
op_doi |
https://doi.org/10.1590/S0037-86822010000100023 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
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43 |
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100 |
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101 |
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