PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples PCR-RFLP do 16S DNA ribossomal para confirmar a identificação de Enterococcus gallinarum e Enterococcus casseliflavus isolados de amostras clínicas e alimentares

INTRODUCTION: This study aimed to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples by PCR-RFLP. METHODS: Fifty-two strains identified by conventional biochemical exams were submitted to PCR amplification and digested with Hi...

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Published in:Revista da Sociedade Brasileira de Medicina Tropical
Main Authors: Aline Weber Medeiros, Pedro d'Azevedo, Rebeca Inhoque Pereira, Ana Paula Cassenego, Sueli Van Der Sand, Jeverson Frazzon, Ana Paula Guedes Frazzon
Format: Article in Journal/Newspaper
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2010
Subjects:
Online Access:https://doi.org/10.1590/S0037-86822010000100023
https://doaj.org/article/118b77cd54824130b16dc7b01ec04d56
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spelling ftdoajarticles:oai:doaj.org/article:118b77cd54824130b16dc7b01ec04d56 2023-05-15T15:13:27+02:00 PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples PCR-RFLP do 16S DNA ribossomal para confirmar a identificação de Enterococcus gallinarum e Enterococcus casseliflavus isolados de amostras clínicas e alimentares Aline Weber Medeiros Pedro d'Azevedo Rebeca Inhoque Pereira Ana Paula Cassenego Sueli Van Der Sand Jeverson Frazzon Ana Paula Guedes Frazzon 2010-02-01T00:00:00Z https://doi.org/10.1590/S0037-86822010000100023 https://doaj.org/article/118b77cd54824130b16dc7b01ec04d56 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822010000100023 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 doi:10.1590/S0037-86822010000100023 0037-8682 1678-9849 https://doaj.org/article/118b77cd54824130b16dc7b01ec04d56 Revista da Sociedade Brasileira de Medicina Tropical, Vol 43, Iss 1, Pp 100-101 (2010) PCR-RFLP de 16S rDNA Enterococcus gallinarum Enterococcus casseliflavus PCR-RFLP of 16S rDNA Arctic medicine. Tropical medicine RC955-962 article 2010 ftdoajarticles https://doi.org/10.1590/S0037-86822010000100023 2022-12-30T23:08:11Z INTRODUCTION: This study aimed to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples by PCR-RFLP. METHODS: Fifty-two strains identified by conventional biochemical exams were submitted to PCR amplification and digested with HinfI. Only 20 (38.5%) of the 52 strains showed a DNA pattern expected for E. gallinarum and E. casseliflavus. RESULTS: Analysis of the results of this study showed that E. gallinarum and E. casseliflavus are occasionally erroneously identified and confirmed the potential application of 16S rDNA analysis for accurate identification of these species. CONCLUSIONS: A correct identification is important to distinguish between intrinsic and acquired vancomycin resistance. INTRODUÇÃO: O objetivo deste estudo foi confirmar a identificação de amostras clínicas e alimentos de Enterococcus gallinarum e Enterococcus casseliflavus por PCR-RFLP. MÉTODOS: Cinquenta e duas cepas identificadas por exames bioquímicos convencionais foram submetidos a amplificação por PCR e digestão com HinfI. Apenas 20 (38,5%) das 52 amostras apresentaram um padrão de DNA esperado E. gallinarum e E. casseliflavus. RESULTADOS: Analise dos resultados deste estudo demonstraram que, algumas vezes E. gallinarum e E. casseliflavus são erroneamente identificados e confirmaram a potencial aplicação da análise do 16S rDNA para identificação exata destas espécies. CONCLUSÕES: A correta identificação é importante a fim de distinguir entre resistência intrínseca e adquirida à vancomicina. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 43 1 100 101
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic PCR-RFLP de 16S rDNA
Enterococcus gallinarum
Enterococcus casseliflavus
PCR-RFLP of 16S rDNA
Arctic medicine. Tropical medicine
RC955-962
spellingShingle PCR-RFLP de 16S rDNA
Enterococcus gallinarum
Enterococcus casseliflavus
PCR-RFLP of 16S rDNA
Arctic medicine. Tropical medicine
RC955-962
Aline Weber Medeiros
Pedro d'Azevedo
Rebeca Inhoque Pereira
Ana Paula Cassenego
Sueli Van Der Sand
Jeverson Frazzon
Ana Paula Guedes Frazzon
PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples PCR-RFLP do 16S DNA ribossomal para confirmar a identificação de Enterococcus gallinarum e Enterococcus casseliflavus isolados de amostras clínicas e alimentares
topic_facet PCR-RFLP de 16S rDNA
Enterococcus gallinarum
Enterococcus casseliflavus
PCR-RFLP of 16S rDNA
Arctic medicine. Tropical medicine
RC955-962
description INTRODUCTION: This study aimed to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples by PCR-RFLP. METHODS: Fifty-two strains identified by conventional biochemical exams were submitted to PCR amplification and digested with HinfI. Only 20 (38.5%) of the 52 strains showed a DNA pattern expected for E. gallinarum and E. casseliflavus. RESULTS: Analysis of the results of this study showed that E. gallinarum and E. casseliflavus are occasionally erroneously identified and confirmed the potential application of 16S rDNA analysis for accurate identification of these species. CONCLUSIONS: A correct identification is important to distinguish between intrinsic and acquired vancomycin resistance. INTRODUÇÃO: O objetivo deste estudo foi confirmar a identificação de amostras clínicas e alimentos de Enterococcus gallinarum e Enterococcus casseliflavus por PCR-RFLP. MÉTODOS: Cinquenta e duas cepas identificadas por exames bioquímicos convencionais foram submetidos a amplificação por PCR e digestão com HinfI. Apenas 20 (38,5%) das 52 amostras apresentaram um padrão de DNA esperado E. gallinarum e E. casseliflavus. RESULTADOS: Analise dos resultados deste estudo demonstraram que, algumas vezes E. gallinarum e E. casseliflavus são erroneamente identificados e confirmaram a potencial aplicação da análise do 16S rDNA para identificação exata destas espécies. CONCLUSÕES: A correta identificação é importante a fim de distinguir entre resistência intrínseca e adquirida à vancomicina.
format Article in Journal/Newspaper
author Aline Weber Medeiros
Pedro d'Azevedo
Rebeca Inhoque Pereira
Ana Paula Cassenego
Sueli Van Der Sand
Jeverson Frazzon
Ana Paula Guedes Frazzon
author_facet Aline Weber Medeiros
Pedro d'Azevedo
Rebeca Inhoque Pereira
Ana Paula Cassenego
Sueli Van Der Sand
Jeverson Frazzon
Ana Paula Guedes Frazzon
author_sort Aline Weber Medeiros
title PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples PCR-RFLP do 16S DNA ribossomal para confirmar a identificação de Enterococcus gallinarum e Enterococcus casseliflavus isolados de amostras clínicas e alimentares
title_short PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples PCR-RFLP do 16S DNA ribossomal para confirmar a identificação de Enterococcus gallinarum e Enterococcus casseliflavus isolados de amostras clínicas e alimentares
title_full PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples PCR-RFLP do 16S DNA ribossomal para confirmar a identificação de Enterococcus gallinarum e Enterococcus casseliflavus isolados de amostras clínicas e alimentares
title_fullStr PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples PCR-RFLP do 16S DNA ribossomal para confirmar a identificação de Enterococcus gallinarum e Enterococcus casseliflavus isolados de amostras clínicas e alimentares
title_full_unstemmed PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples PCR-RFLP do 16S DNA ribossomal para confirmar a identificação de Enterococcus gallinarum e Enterococcus casseliflavus isolados de amostras clínicas e alimentares
title_sort pcr-rflp of 16s ribosomal dna to confirm the identification of enterococcus gallinarum and enterococcus casseliflavus isolated from clinical and food samples pcr-rflp do 16s dna ribossomal para confirmar a identificação de enterococcus gallinarum e enterococcus casseliflavus isolados de amostras clínicas e alimentares
publisher Sociedade Brasileira de Medicina Tropical (SBMT)
publishDate 2010
url https://doi.org/10.1590/S0037-86822010000100023
https://doaj.org/article/118b77cd54824130b16dc7b01ec04d56
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source Revista da Sociedade Brasileira de Medicina Tropical, Vol 43, Iss 1, Pp 100-101 (2010)
op_relation http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822010000100023
https://doaj.org/toc/0037-8682
https://doaj.org/toc/1678-9849
doi:10.1590/S0037-86822010000100023
0037-8682
1678-9849
https://doaj.org/article/118b77cd54824130b16dc7b01ec04d56
op_doi https://doi.org/10.1590/S0037-86822010000100023
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