The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40.
Background Hepatic stellate cells (HSCs) are one of the main cell types involved in liver fibrosis induced by many factors, including schistosomes. Previous studies in our lab have shown that recombinant P40 protein from Schistosoma japonicum (rSjP40) can inhibit HSC activation in vitro. Let-7b is a...
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ftdoajarticles:oai:doaj.org/article:117b2bf55c2043a7b8982840eae57e84 2023-05-15T15:08:32+02:00 The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40. Xiaolei Sun Li Zhang Yuting Jiang Aihong Li Dandan Zhu Jiangrong Wu Yinong Duan 2021-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009472 https://doaj.org/article/117b2bf55c2043a7b8982840eae57e84 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009472 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009472 https://doaj.org/article/117b2bf55c2043a7b8982840eae57e84 PLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009472 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009472 2022-12-31T11:13:25Z Background Hepatic stellate cells (HSCs) are one of the main cell types involved in liver fibrosis induced by many factors, including schistosomes. Previous studies in our lab have shown that recombinant P40 protein from Schistosoma japonicum (rSjP40) can inhibit HSC activation in vitro. Let-7b is a member of the let-7 microRNA family and plays an inhibitory role in a variety of diseases and inflammatory conditions. In this study, we investigated the role of let-7b in the inhibition of HSC activation by rSjP40. Methods Expression of let-7b was detected by quantitative real-time PCR. A dual luciferase assay was used to confirm direct interaction between let-7b and collagen I. We also used western blot to assess protein levels of TGFβRI and collagen type I α1 (COL1A1). Results We found that rSjP40 up-regulates expression of let-7b in HSCs. Let-7b inhibits collagen I expression by directly targeting the 3'UTR region of the collagen I gene. Furthermore, we discovered that let-7b inhibitor partially restores the loss of collagen I expression caused by rSjP40. Conclusion Our research clarifies the role of let-7b in the inhibition of HSC activation by rSjP40 and will provide new insights and ideas for the inhibition of HSC activation and treatment of liver fibrosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 6 e0009472 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Xiaolei Sun Li Zhang Yuting Jiang Aihong Li Dandan Zhu Jiangrong Wu Yinong Duan The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Hepatic stellate cells (HSCs) are one of the main cell types involved in liver fibrosis induced by many factors, including schistosomes. Previous studies in our lab have shown that recombinant P40 protein from Schistosoma japonicum (rSjP40) can inhibit HSC activation in vitro. Let-7b is a member of the let-7 microRNA family and plays an inhibitory role in a variety of diseases and inflammatory conditions. In this study, we investigated the role of let-7b in the inhibition of HSC activation by rSjP40. Methods Expression of let-7b was detected by quantitative real-time PCR. A dual luciferase assay was used to confirm direct interaction between let-7b and collagen I. We also used western blot to assess protein levels of TGFβRI and collagen type I α1 (COL1A1). Results We found that rSjP40 up-regulates expression of let-7b in HSCs. Let-7b inhibits collagen I expression by directly targeting the 3'UTR region of the collagen I gene. Furthermore, we discovered that let-7b inhibitor partially restores the loss of collagen I expression caused by rSjP40. Conclusion Our research clarifies the role of let-7b in the inhibition of HSC activation by rSjP40 and will provide new insights and ideas for the inhibition of HSC activation and treatment of liver fibrosis. |
format |
Article in Journal/Newspaper |
author |
Xiaolei Sun Li Zhang Yuting Jiang Aihong Li Dandan Zhu Jiangrong Wu Yinong Duan |
author_facet |
Xiaolei Sun Li Zhang Yuting Jiang Aihong Li Dandan Zhu Jiangrong Wu Yinong Duan |
author_sort |
Xiaolei Sun |
title |
The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40. |
title_short |
The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40. |
title_full |
The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40. |
title_fullStr |
The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40. |
title_full_unstemmed |
The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40. |
title_sort |
role of let-7b in the inhibition of hepatic stellate cell activation by rsjp40. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009472 https://doaj.org/article/117b2bf55c2043a7b8982840eae57e84 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009472 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009472 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009472 https://doaj.org/article/117b2bf55c2043a7b8982840eae57e84 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009472 |
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PLOS Neglected Tropical Diseases |
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15 |
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6 |
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e0009472 |
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