Toll-like receptor 3 gene polymorphisms are not associated with the risk of hepatitis B and hepatitis C virus infection
INTRODUCTION: The present study investigated the prevalence of two single-nucleotide polymorphisms (SNPs) in the Toll-like receptor 3 (TLR3) gene in patients infected with hepatitis B virus (HBV) and hepatitis C virus (HCV). METHODS: Samples collected from HCV (n = 74) and HBV (n = 35) carriers were...
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ftdoajarticles:oai:doaj.org/article:116ee9b4be5f48ea9b485950b530e77c 2023-05-15T15:09:05+02:00 Toll-like receptor 3 gene polymorphisms are not associated with the risk of hepatitis B and hepatitis C virus infection Keyla Santos Guedes de Sá Orlando de Souza Pires-Neto Barbara Brasil Santana Samara Tatielle Monteiro Gomes Ednelza da Silva Graça Amoras Simone Regina da Silva Conde Sâmia Demachki Vânia Nakauth Azevedo Luiz Fernando Almeida Machado Rosimar Neris Martins-Feitosa Marluísa de Oliveira Guimarães Ishak Ricardo Ishak Antonio Carlos Rosário Vallinoto 2015-04-01T00:00:00Z https://doi.org/10.1590/0037-8682-0008-2015 https://doaj.org/article/116ee9b4be5f48ea9b485950b530e77c EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000200136&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0008-2015 https://doaj.org/article/116ee9b4be5f48ea9b485950b530e77c Revista da Sociedade Brasileira de Medicina Tropical, Vol 48, Iss 2, Pp 136-142 (2015) TLR3 rs5743305 rs3775291 HCV HBV Arctic medicine. Tropical medicine RC955-962 article 2015 ftdoajarticles https://doi.org/10.1590/0037-8682-0008-2015 2022-12-30T23:25:46Z INTRODUCTION: The present study investigated the prevalence of two single-nucleotide polymorphisms (SNPs) in the Toll-like receptor 3 (TLR3) gene in patients infected with hepatitis B virus (HBV) and hepatitis C virus (HCV). METHODS: Samples collected from HCV (n = 74) and HBV (n = 35) carriers were subjected to quantitative real-time PCR (qPCR) to detect the presence of the SNPs rs5743305 and rs3775291 in TLR3 and to measure the following biomarkers: alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), and prothrombin time (PT). A healthy control group was investigated and consisted of 299 HCV- and HBV-seronegative individuals. RESULTS: No significant differences in allele, genotype and haplotype frequencies were observed between the investigated groups, and no association was observed between the polymorphisms and histopathological results. Nevertheless, genotypes TA/AA (rs5743305) and GG (rs3775291) appear to be associated with higher levels of ALT (p<0.01), AST (p<0.05) and PT (p<0.05). In addition, genotypes TT (rs5743305; p<0.05) and GG (rs3775291; p<0.05) were associated with higher GGT levels. CONCLUSIONS: This genetic analysis revealed the absence of an association between the polymorphisms investigated and susceptibility to HBV and HCV infection; however, these polymorphisms might be associated with a greater degree of biliary damage during the course of HCV infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 48 2 136 142 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
TLR3 rs5743305 rs3775291 HCV HBV Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
TLR3 rs5743305 rs3775291 HCV HBV Arctic medicine. Tropical medicine RC955-962 Keyla Santos Guedes de Sá Orlando de Souza Pires-Neto Barbara Brasil Santana Samara Tatielle Monteiro Gomes Ednelza da Silva Graça Amoras Simone Regina da Silva Conde Sâmia Demachki Vânia Nakauth Azevedo Luiz Fernando Almeida Machado Rosimar Neris Martins-Feitosa Marluísa de Oliveira Guimarães Ishak Ricardo Ishak Antonio Carlos Rosário Vallinoto Toll-like receptor 3 gene polymorphisms are not associated with the risk of hepatitis B and hepatitis C virus infection |
topic_facet |
TLR3 rs5743305 rs3775291 HCV HBV Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: The present study investigated the prevalence of two single-nucleotide polymorphisms (SNPs) in the Toll-like receptor 3 (TLR3) gene in patients infected with hepatitis B virus (HBV) and hepatitis C virus (HCV). METHODS: Samples collected from HCV (n = 74) and HBV (n = 35) carriers were subjected to quantitative real-time PCR (qPCR) to detect the presence of the SNPs rs5743305 and rs3775291 in TLR3 and to measure the following biomarkers: alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), and prothrombin time (PT). A healthy control group was investigated and consisted of 299 HCV- and HBV-seronegative individuals. RESULTS: No significant differences in allele, genotype and haplotype frequencies were observed between the investigated groups, and no association was observed between the polymorphisms and histopathological results. Nevertheless, genotypes TA/AA (rs5743305) and GG (rs3775291) appear to be associated with higher levels of ALT (p<0.01), AST (p<0.05) and PT (p<0.05). In addition, genotypes TT (rs5743305; p<0.05) and GG (rs3775291; p<0.05) were associated with higher GGT levels. CONCLUSIONS: This genetic analysis revealed the absence of an association between the polymorphisms investigated and susceptibility to HBV and HCV infection; however, these polymorphisms might be associated with a greater degree of biliary damage during the course of HCV infection. |
format |
Article in Journal/Newspaper |
author |
Keyla Santos Guedes de Sá Orlando de Souza Pires-Neto Barbara Brasil Santana Samara Tatielle Monteiro Gomes Ednelza da Silva Graça Amoras Simone Regina da Silva Conde Sâmia Demachki Vânia Nakauth Azevedo Luiz Fernando Almeida Machado Rosimar Neris Martins-Feitosa Marluísa de Oliveira Guimarães Ishak Ricardo Ishak Antonio Carlos Rosário Vallinoto |
author_facet |
Keyla Santos Guedes de Sá Orlando de Souza Pires-Neto Barbara Brasil Santana Samara Tatielle Monteiro Gomes Ednelza da Silva Graça Amoras Simone Regina da Silva Conde Sâmia Demachki Vânia Nakauth Azevedo Luiz Fernando Almeida Machado Rosimar Neris Martins-Feitosa Marluísa de Oliveira Guimarães Ishak Ricardo Ishak Antonio Carlos Rosário Vallinoto |
author_sort |
Keyla Santos Guedes de Sá |
title |
Toll-like receptor 3 gene polymorphisms are not associated with the risk of hepatitis B and hepatitis C virus infection |
title_short |
Toll-like receptor 3 gene polymorphisms are not associated with the risk of hepatitis B and hepatitis C virus infection |
title_full |
Toll-like receptor 3 gene polymorphisms are not associated with the risk of hepatitis B and hepatitis C virus infection |
title_fullStr |
Toll-like receptor 3 gene polymorphisms are not associated with the risk of hepatitis B and hepatitis C virus infection |
title_full_unstemmed |
Toll-like receptor 3 gene polymorphisms are not associated with the risk of hepatitis B and hepatitis C virus infection |
title_sort |
toll-like receptor 3 gene polymorphisms are not associated with the risk of hepatitis b and hepatitis c virus infection |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2015 |
url |
https://doi.org/10.1590/0037-8682-0008-2015 https://doaj.org/article/116ee9b4be5f48ea9b485950b530e77c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 48, Iss 2, Pp 136-142 (2015) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000200136&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0008-2015 https://doaj.org/article/116ee9b4be5f48ea9b485950b530e77c |
op_doi |
https://doi.org/10.1590/0037-8682-0008-2015 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
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48 |
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2 |
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136 |
op_container_end_page |
142 |
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