Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp.
Leptospirosis is a bacterial zoonosis, caused by Leptospira spp., that leads to significant morbidity and mortality worldwide. Despite considerable advances, much is yet to be discovered about disease pathogenicity. The influence of epigenetic mechanisms, particularly RNA-mediated post-transcription...
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ftdoajarticles:oai:doaj.org/article:114ec55113b94d10af70caabbdf2ec7b 2023-05-15T15:05:01+02:00 Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp. Leandro Encarnação Garcia Erivelto Corrêa de Araújo Junior Larissa Martins Melo Jaqueline Poleto Bragato Juliana Regina Peiró Valéria Marçal Félix de Lima Márcia Marinho Daniel Robert Arnold Flavia Lombardi Lopes 2018-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006621 https://doaj.org/article/114ec55113b94d10af70caabbdf2ec7b EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6051669?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006621 https://doaj.org/article/114ec55113b94d10af70caabbdf2ec7b PLoS Neglected Tropical Diseases, Vol 12, Iss 7, p e0006621 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006621 2022-12-31T00:59:18Z Leptospirosis is a bacterial zoonosis, caused by Leptospira spp., that leads to significant morbidity and mortality worldwide. Despite considerable advances, much is yet to be discovered about disease pathogenicity. The influence of epigenetic mechanisms, particularly RNA-mediated post-transcriptional regulation of host immune response has been described following a variety of bacterial infections. The current study examined the microtranscriptome of macrophages J774A.1 following an 8h infection with virulent, attenuated and saprophyte strains of Leptospira. Microarray analysis revealed that 29 miRNAs were misregulated following leptospiral infection compared to control macrophages in a strain and virulence-specific manner. Pathway analysis for targets of these differentially expressed miRNAs suggests that several processes involved in immune response could be regulated by miRNAs. Our data provides the first evidence that host miRNAs are regulated by Leptospira infection in macrophages. A number of the identified miRNA targets participate in key immune response processes. We suggest that post-transcriptional regulation by miRNAs may play a role in host response to infection in leptospirosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 7 e0006621 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Leandro Encarnação Garcia Erivelto Corrêa de Araújo Junior Larissa Martins Melo Jaqueline Poleto Bragato Juliana Regina Peiró Valéria Marçal Félix de Lima Márcia Marinho Daniel Robert Arnold Flavia Lombardi Lopes Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Leptospirosis is a bacterial zoonosis, caused by Leptospira spp., that leads to significant morbidity and mortality worldwide. Despite considerable advances, much is yet to be discovered about disease pathogenicity. The influence of epigenetic mechanisms, particularly RNA-mediated post-transcriptional regulation of host immune response has been described following a variety of bacterial infections. The current study examined the microtranscriptome of macrophages J774A.1 following an 8h infection with virulent, attenuated and saprophyte strains of Leptospira. Microarray analysis revealed that 29 miRNAs were misregulated following leptospiral infection compared to control macrophages in a strain and virulence-specific manner. Pathway analysis for targets of these differentially expressed miRNAs suggests that several processes involved in immune response could be regulated by miRNAs. Our data provides the first evidence that host miRNAs are regulated by Leptospira infection in macrophages. A number of the identified miRNA targets participate in key immune response processes. We suggest that post-transcriptional regulation by miRNAs may play a role in host response to infection in leptospirosis. |
format |
Article in Journal/Newspaper |
author |
Leandro Encarnação Garcia Erivelto Corrêa de Araújo Junior Larissa Martins Melo Jaqueline Poleto Bragato Juliana Regina Peiró Valéria Marçal Félix de Lima Márcia Marinho Daniel Robert Arnold Flavia Lombardi Lopes |
author_facet |
Leandro Encarnação Garcia Erivelto Corrêa de Araújo Junior Larissa Martins Melo Jaqueline Poleto Bragato Juliana Regina Peiró Valéria Marçal Félix de Lima Márcia Marinho Daniel Robert Arnold Flavia Lombardi Lopes |
author_sort |
Leandro Encarnação Garcia |
title |
Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp. |
title_short |
Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp. |
title_full |
Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp. |
title_fullStr |
Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp. |
title_full_unstemmed |
Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp. |
title_sort |
characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of leptospira spp. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006621 https://doaj.org/article/114ec55113b94d10af70caabbdf2ec7b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 7, p e0006621 (2018) |
op_relation |
http://europepmc.org/articles/PMC6051669?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006621 https://doaj.org/article/114ec55113b94d10af70caabbdf2ec7b |
op_doi |
https://doi.org/10.1371/journal.pntd.0006621 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
7 |
container_start_page |
e0006621 |
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1766336782716108800 |