Production and Chemical Characterization of Exopolysaccharides by Antarctic Yeasts Vishniacozyma victoriae and Tremellomycetes sp.
The study aimed to investigate exopolysaccharides (EPSs) produced by two Antarctic yeasts isolated from Livingston Island. The species were identified as Vishniacozyma victoriae (V) and Tremellomycetes sp. (T) based on a molecular genetic analysis of ITS1-5.8S-ITS4 regions of the 18S rRNA gene. The...
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ftdoajarticles:oai:doaj.org/article:1114ac0ded154ab4ad4cd697252ced0c 2023-05-15T14:02:10+02:00 Production and Chemical Characterization of Exopolysaccharides by Antarctic Yeasts Vishniacozyma victoriae and Tremellomycetes sp. Snezhana Rusinova-Videva Manol Ognyanov Yordan Georgiev Margarita Kambourova Aleksandar Adamov Vasilena Krasteva 2022-02-01T00:00:00Z https://doi.org/10.3390/app12041805 https://doaj.org/article/1114ac0ded154ab4ad4cd697252ced0c EN eng MDPI AG https://www.mdpi.com/2076-3417/12/4/1805 https://doaj.org/toc/2076-3417 doi:10.3390/app12041805 2076-3417 https://doaj.org/article/1114ac0ded154ab4ad4cd697252ced0c Applied Sciences, Vol 12, Iss 1805, p 1805 (2022) Antarctic yeasts polysaccharides biotechnology chromatography spectroscopy Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 article 2022 ftdoajarticles https://doi.org/10.3390/app12041805 2022-12-31T15:11:12Z The study aimed to investigate exopolysaccharides (EPSs) produced by two Antarctic yeasts isolated from Livingston Island. The species were identified as Vishniacozyma victoriae (V) and Tremellomycetes sp. (T) based on a molecular genetic analysis of ITS1-5.8S-ITS4 regions of the 18S rRNA gene. The EPS production was investigated under stress conditions in culture flasks and a bioreactor. Different chromatographic (HPLC-RID, HPSEC-RID) and spectral (FT-IR) analyses were employed to characterize EPSs. Tremellomycetes sp. accumulated 7 g/L biomass and 4.5 g/L EPS after 120 h of cultivation. The total carbohydrate content of V-EPS and T-EPS was 75.4% and 79.0%, respectively. The EPSs mainly consisted of mannose (30–32%), which was followed by glucose, xylose, galactose, and small amounts of uronic acids (6.3–7.0%). EPSs had appreciable amounts of proteins (11–12%). The FT-IR spectra contained absorption bands typical for hetero-mannans and β -glucans (797–1033 cm −1 ). EPSs were heterogeneous with a broad molecular weight distribution range (47 × 10 4 –68 × 10 4 g/mol). In conclusion, both yeasts synthesized high-molecular-weight heteromannans, and Tremellomycetes sp. stood out as being a better producer than V. victoriae . The current study also formed a basis for a better assessment of the potential for practical application of EPSs and yeasts in biochemical engineering and biotechnology. Article in Journal/Newspaper Antarc* Antarctic Livingston Island Directory of Open Access Journals: DOAJ Articles Antarctic Livingston Island ENVELOPE(-60.500,-60.500,-62.600,-62.600) Applied Sciences 12 4 1805 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctic yeasts polysaccharides biotechnology chromatography spectroscopy Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 |
spellingShingle |
Antarctic yeasts polysaccharides biotechnology chromatography spectroscopy Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Snezhana Rusinova-Videva Manol Ognyanov Yordan Georgiev Margarita Kambourova Aleksandar Adamov Vasilena Krasteva Production and Chemical Characterization of Exopolysaccharides by Antarctic Yeasts Vishniacozyma victoriae and Tremellomycetes sp. |
topic_facet |
Antarctic yeasts polysaccharides biotechnology chromatography spectroscopy Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 |
description |
The study aimed to investigate exopolysaccharides (EPSs) produced by two Antarctic yeasts isolated from Livingston Island. The species were identified as Vishniacozyma victoriae (V) and Tremellomycetes sp. (T) based on a molecular genetic analysis of ITS1-5.8S-ITS4 regions of the 18S rRNA gene. The EPS production was investigated under stress conditions in culture flasks and a bioreactor. Different chromatographic (HPLC-RID, HPSEC-RID) and spectral (FT-IR) analyses were employed to characterize EPSs. Tremellomycetes sp. accumulated 7 g/L biomass and 4.5 g/L EPS after 120 h of cultivation. The total carbohydrate content of V-EPS and T-EPS was 75.4% and 79.0%, respectively. The EPSs mainly consisted of mannose (30–32%), which was followed by glucose, xylose, galactose, and small amounts of uronic acids (6.3–7.0%). EPSs had appreciable amounts of proteins (11–12%). The FT-IR spectra contained absorption bands typical for hetero-mannans and β -glucans (797–1033 cm −1 ). EPSs were heterogeneous with a broad molecular weight distribution range (47 × 10 4 –68 × 10 4 g/mol). In conclusion, both yeasts synthesized high-molecular-weight heteromannans, and Tremellomycetes sp. stood out as being a better producer than V. victoriae . The current study also formed a basis for a better assessment of the potential for practical application of EPSs and yeasts in biochemical engineering and biotechnology. |
format |
Article in Journal/Newspaper |
author |
Snezhana Rusinova-Videva Manol Ognyanov Yordan Georgiev Margarita Kambourova Aleksandar Adamov Vasilena Krasteva |
author_facet |
Snezhana Rusinova-Videva Manol Ognyanov Yordan Georgiev Margarita Kambourova Aleksandar Adamov Vasilena Krasteva |
author_sort |
Snezhana Rusinova-Videva |
title |
Production and Chemical Characterization of Exopolysaccharides by Antarctic Yeasts Vishniacozyma victoriae and Tremellomycetes sp. |
title_short |
Production and Chemical Characterization of Exopolysaccharides by Antarctic Yeasts Vishniacozyma victoriae and Tremellomycetes sp. |
title_full |
Production and Chemical Characterization of Exopolysaccharides by Antarctic Yeasts Vishniacozyma victoriae and Tremellomycetes sp. |
title_fullStr |
Production and Chemical Characterization of Exopolysaccharides by Antarctic Yeasts Vishniacozyma victoriae and Tremellomycetes sp. |
title_full_unstemmed |
Production and Chemical Characterization of Exopolysaccharides by Antarctic Yeasts Vishniacozyma victoriae and Tremellomycetes sp. |
title_sort |
production and chemical characterization of exopolysaccharides by antarctic yeasts vishniacozyma victoriae and tremellomycetes sp. |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/app12041805 https://doaj.org/article/1114ac0ded154ab4ad4cd697252ced0c |
long_lat |
ENVELOPE(-60.500,-60.500,-62.600,-62.600) |
geographic |
Antarctic Livingston Island |
geographic_facet |
Antarctic Livingston Island |
genre |
Antarc* Antarctic Livingston Island |
genre_facet |
Antarc* Antarctic Livingston Island |
op_source |
Applied Sciences, Vol 12, Iss 1805, p 1805 (2022) |
op_relation |
https://www.mdpi.com/2076-3417/12/4/1805 https://doaj.org/toc/2076-3417 doi:10.3390/app12041805 2076-3417 https://doaj.org/article/1114ac0ded154ab4ad4cd697252ced0c |
op_doi |
https://doi.org/10.3390/app12041805 |
container_title |
Applied Sciences |
container_volume |
12 |
container_issue |
4 |
container_start_page |
1805 |
_version_ |
1766272274247188480 |