A novel activity on thymocytes cells exerted by the rattlesnake (Crotalus durissus cumanensis) venom
Introduction: The thymus is active mainly during the neonatal and pre-adolescent periods. Objective: To test naïve thymocytes proliferation and monocytes stimulation. Materials and methods: We collected fresh thymus tissue from neonate mice after surgery. Suspension cells were coated onto Ficoll-Hyp...
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Instituto Nacional de Salud
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ftdoajarticles:oai:doaj.org/article:10dfa68b3bb64a248ab20e34861152c8 2023-05-15T15:08:12+02:00 A novel activity on thymocytes cells exerted by the rattlesnake (Crotalus durissus cumanensis) venom María M. Pulido-Méndez Elvia Azuaje Alexis Rodríguez-Acosta 2021-09-01T00:00:00Z https://doi.org/10.7705/biomedica.5599 https://doaj.org/article/10dfa68b3bb64a248ab20e34861152c8 EN ES eng spa Instituto Nacional de Salud https://revistabiomedica.org/index.php/biomedica/article/view/5599 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.5599 https://doaj.org/article/10dfa68b3bb64a248ab20e34861152c8 Biomédica: revista del Instituto Nacional de Salud, Vol 41, Iss 3, Pp 449-457 (2021) crotalus crotalid venoms thymocytes monocytes macrophages venom Medicine R Arctic medicine. Tropical medicine RC955-962 article 2021 ftdoajarticles https://doi.org/10.7705/biomedica.5599 2022-12-31T00:57:09Z Introduction: The thymus is active mainly during the neonatal and pre-adolescent periods. Objective: To test naïve thymocytes proliferation and monocytes stimulation. Materials and methods: We collected fresh thymus tissue from neonate mice after surgery. Suspension cells were coated onto Ficoll-Hypaque support. The obtained cells (thymocytes) were cultured measuring the proliferation of naïve T cells stimulated by Crotalus durissus cumanensis (Cdc) venom at sub-lethal doses (20 ng). Then, we supplemented the wells with AlamarBlue™ and incubated them for 5 h to test their proliferation. Mononuclear cells from mice peripheral blood were collected and layered onto the support of the Ficoll-Hypaque solution. We added the thymocytes actively dividing (25 x 105 cells) from cultures stimulated with Cdc venom at 20 ng/well to cultured monocytes freshly obtained from the Ficoll-Hypaque separation. Both cell populations were incubated for 36 h until monocytes matured to macrophages. Results: The naïve thymocytes rapidly proliferated after stimulation with the Cdc venom (NTCdc) and these successively induced the maturation and function of monocytes progenitor cells to mature macrophages, which ingested Chinese ink. Conclusions: The naïve thymocytes proliferated by stimulation with the Cdc venom and subsequently the NT/Cdc induced the rapid maturation and function of monocytes progenitor cells becoming mature macrophages with their phenotypic characteristics. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 41 3 449 457 |
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crotalus crotalid venoms thymocytes monocytes macrophages venom Medicine R Arctic medicine. Tropical medicine RC955-962 |
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crotalus crotalid venoms thymocytes monocytes macrophages venom Medicine R Arctic medicine. Tropical medicine RC955-962 María M. Pulido-Méndez Elvia Azuaje Alexis Rodríguez-Acosta A novel activity on thymocytes cells exerted by the rattlesnake (Crotalus durissus cumanensis) venom |
topic_facet |
crotalus crotalid venoms thymocytes monocytes macrophages venom Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: The thymus is active mainly during the neonatal and pre-adolescent periods. Objective: To test naïve thymocytes proliferation and monocytes stimulation. Materials and methods: We collected fresh thymus tissue from neonate mice after surgery. Suspension cells were coated onto Ficoll-Hypaque support. The obtained cells (thymocytes) were cultured measuring the proliferation of naïve T cells stimulated by Crotalus durissus cumanensis (Cdc) venom at sub-lethal doses (20 ng). Then, we supplemented the wells with AlamarBlue™ and incubated them for 5 h to test their proliferation. Mononuclear cells from mice peripheral blood were collected and layered onto the support of the Ficoll-Hypaque solution. We added the thymocytes actively dividing (25 x 105 cells) from cultures stimulated with Cdc venom at 20 ng/well to cultured monocytes freshly obtained from the Ficoll-Hypaque separation. Both cell populations were incubated for 36 h until monocytes matured to macrophages. Results: The naïve thymocytes rapidly proliferated after stimulation with the Cdc venom (NTCdc) and these successively induced the maturation and function of monocytes progenitor cells to mature macrophages, which ingested Chinese ink. Conclusions: The naïve thymocytes proliferated by stimulation with the Cdc venom and subsequently the NT/Cdc induced the rapid maturation and function of monocytes progenitor cells becoming mature macrophages with their phenotypic characteristics. |
format |
Article in Journal/Newspaper |
author |
María M. Pulido-Méndez Elvia Azuaje Alexis Rodríguez-Acosta |
author_facet |
María M. Pulido-Méndez Elvia Azuaje Alexis Rodríguez-Acosta |
author_sort |
María M. Pulido-Méndez |
title |
A novel activity on thymocytes cells exerted by the rattlesnake (Crotalus durissus cumanensis) venom |
title_short |
A novel activity on thymocytes cells exerted by the rattlesnake (Crotalus durissus cumanensis) venom |
title_full |
A novel activity on thymocytes cells exerted by the rattlesnake (Crotalus durissus cumanensis) venom |
title_fullStr |
A novel activity on thymocytes cells exerted by the rattlesnake (Crotalus durissus cumanensis) venom |
title_full_unstemmed |
A novel activity on thymocytes cells exerted by the rattlesnake (Crotalus durissus cumanensis) venom |
title_sort |
novel activity on thymocytes cells exerted by the rattlesnake (crotalus durissus cumanensis) venom |
publisher |
Instituto Nacional de Salud |
publishDate |
2021 |
url |
https://doi.org/10.7705/biomedica.5599 https://doaj.org/article/10dfa68b3bb64a248ab20e34861152c8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 41, Iss 3, Pp 449-457 (2021) |
op_relation |
https://revistabiomedica.org/index.php/biomedica/article/view/5599 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.5599 https://doaj.org/article/10dfa68b3bb64a248ab20e34861152c8 |
op_doi |
https://doi.org/10.7705/biomedica.5599 |
container_title |
Biomédica |
container_volume |
41 |
container_issue |
3 |
container_start_page |
449 |
op_container_end_page |
457 |
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1766339605754281984 |