Expression profiling of Echinococcus multilocularis miRNAs throughout metacestode development in vitro.
The neglected zoonotic disease alveolar echinococcosis (AE) is caused by the metacestode stage of the tapeworm parasite Echinococcus multilocularis. MicroRNAs (miRNAs) are small non-coding RNAs with a major role in regulating gene expression in key biological processes. We analyzed the expression pr...
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ftdoajarticles:oai:doaj.org/article:10d119d5d4e141e3ba4ad2a3a9f37c10 2023-05-15T15:16:54+02:00 Expression profiling of Echinococcus multilocularis miRNAs throughout metacestode development in vitro. Natalia Macchiaroli Matías Preza Matías Gastón Pérez Laura Kamenetzky Marcela Cucher Uriel Koziol Estela Castillo Matthew Berriman Klaus Brehm Mara Cecilia Rosenzvit 2021-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009297 https://doaj.org/article/10d119d5d4e141e3ba4ad2a3a9f37c10 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009297 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009297 https://doaj.org/article/10d119d5d4e141e3ba4ad2a3a9f37c10 PLoS Neglected Tropical Diseases, Vol 15, Iss 3, p e0009297 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009297 2022-12-31T14:48:54Z The neglected zoonotic disease alveolar echinococcosis (AE) is caused by the metacestode stage of the tapeworm parasite Echinococcus multilocularis. MicroRNAs (miRNAs) are small non-coding RNAs with a major role in regulating gene expression in key biological processes. We analyzed the expression profile of E. multilocularis miRNAs throughout metacestode development in vitro, determined the spatial expression of miR-71 in metacestodes cultured in vitro and predicted miRNA targets. Small cDNA libraries from different samples of E. multilocularis were sequenced. We confirmed the expression of 37 miRNAs in E. multilocularis being some of them absent in the host, such as miR-71. We found a few miRNAs highly expressed in all life cycle stages and conditions analyzed, whereas most miRNAs showed very low expression. The most expressed miRNAs were miR-71, miR-9, let-7, miR-10, miR-4989 and miR-1. The high expression of these miRNAs was conserved in other tapeworms, suggesting essential roles in development, survival, or host-parasite interaction. We found highly regulated miRNAs during the different transitions or cultured conditions analyzed, which might suggest a role in the regulation of developmental timing, host-parasite interaction, and/or in maintaining the unique developmental features of each developmental stage or condition. We determined that miR-71 is expressed in germinative cells and in other cell types of the germinal layer in E. multilocularis metacestodes cultured in vitro. MiRNA target prediction of the most highly expressed miRNAs and in silico functional analysis suggested conserved and essential roles for these miRNAs in parasite biology. We found relevant targets potentially involved in development, cell growth and death, lifespan regulation, transcription, signal transduction and cell motility. The evolutionary conservation and expression analyses of E. multilocularis miRNAs throughout metacestode development along with the in silico functional analyses of their predicted targets might help to ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 3 e0009297 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Natalia Macchiaroli Matías Preza Matías Gastón Pérez Laura Kamenetzky Marcela Cucher Uriel Koziol Estela Castillo Matthew Berriman Klaus Brehm Mara Cecilia Rosenzvit Expression profiling of Echinococcus multilocularis miRNAs throughout metacestode development in vitro. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The neglected zoonotic disease alveolar echinococcosis (AE) is caused by the metacestode stage of the tapeworm parasite Echinococcus multilocularis. MicroRNAs (miRNAs) are small non-coding RNAs with a major role in regulating gene expression in key biological processes. We analyzed the expression profile of E. multilocularis miRNAs throughout metacestode development in vitro, determined the spatial expression of miR-71 in metacestodes cultured in vitro and predicted miRNA targets. Small cDNA libraries from different samples of E. multilocularis were sequenced. We confirmed the expression of 37 miRNAs in E. multilocularis being some of them absent in the host, such as miR-71. We found a few miRNAs highly expressed in all life cycle stages and conditions analyzed, whereas most miRNAs showed very low expression. The most expressed miRNAs were miR-71, miR-9, let-7, miR-10, miR-4989 and miR-1. The high expression of these miRNAs was conserved in other tapeworms, suggesting essential roles in development, survival, or host-parasite interaction. We found highly regulated miRNAs during the different transitions or cultured conditions analyzed, which might suggest a role in the regulation of developmental timing, host-parasite interaction, and/or in maintaining the unique developmental features of each developmental stage or condition. We determined that miR-71 is expressed in germinative cells and in other cell types of the germinal layer in E. multilocularis metacestodes cultured in vitro. MiRNA target prediction of the most highly expressed miRNAs and in silico functional analysis suggested conserved and essential roles for these miRNAs in parasite biology. We found relevant targets potentially involved in development, cell growth and death, lifespan regulation, transcription, signal transduction and cell motility. The evolutionary conservation and expression analyses of E. multilocularis miRNAs throughout metacestode development along with the in silico functional analyses of their predicted targets might help to ... |
format |
Article in Journal/Newspaper |
author |
Natalia Macchiaroli Matías Preza Matías Gastón Pérez Laura Kamenetzky Marcela Cucher Uriel Koziol Estela Castillo Matthew Berriman Klaus Brehm Mara Cecilia Rosenzvit |
author_facet |
Natalia Macchiaroli Matías Preza Matías Gastón Pérez Laura Kamenetzky Marcela Cucher Uriel Koziol Estela Castillo Matthew Berriman Klaus Brehm Mara Cecilia Rosenzvit |
author_sort |
Natalia Macchiaroli |
title |
Expression profiling of Echinococcus multilocularis miRNAs throughout metacestode development in vitro. |
title_short |
Expression profiling of Echinococcus multilocularis miRNAs throughout metacestode development in vitro. |
title_full |
Expression profiling of Echinococcus multilocularis miRNAs throughout metacestode development in vitro. |
title_fullStr |
Expression profiling of Echinococcus multilocularis miRNAs throughout metacestode development in vitro. |
title_full_unstemmed |
Expression profiling of Echinococcus multilocularis miRNAs throughout metacestode development in vitro. |
title_sort |
expression profiling of echinococcus multilocularis mirnas throughout metacestode development in vitro. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009297 https://doaj.org/article/10d119d5d4e141e3ba4ad2a3a9f37c10 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 3, p e0009297 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009297 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009297 https://doaj.org/article/10d119d5d4e141e3ba4ad2a3a9f37c10 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009297 |
container_title |
PLOS Neglected Tropical Diseases |
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15 |
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3 |
container_start_page |
e0009297 |
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1766347196957982720 |