Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand
Abstract Objective The main objective of this study was to compare the performance of nested PCR with expert microscopy as a means of detecting Plasmodium parasites during active malaria surveillance in western Thailand. Methods The study was performed from May 2000 to April 2002 in the village of K...
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ftdoajarticles:oai:doaj.org/article:10cf4d4554f044a79e625936609fade7 2023-05-15T15:10:30+02:00 Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand Miller Robert Zollner Gabriela Rachapaew Nattawan Kengluecha Ampornpan Tippayachai Bousaraporn Maneechai Nongnuj Promstaporm Sommai Sattabongkot Jetsumon Coleman Russell E Vaughan Jefferson A Thimasarn Krongtong Khuntirat Benjawan 2006-12-01T00:00:00Z https://doi.org/10.1186/1475-2875-5-121 https://doaj.org/article/10cf4d4554f044a79e625936609fade7 EN eng BMC http://www.malariajournal.com/content/5/1/121 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-5-121 1475-2875 https://doaj.org/article/10cf4d4554f044a79e625936609fade7 Malaria Journal, Vol 5, Iss 1, p 121 (2006) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2006 ftdoajarticles https://doi.org/10.1186/1475-2875-5-121 2022-12-31T04:30:07Z Abstract Objective The main objective of this study was to compare the performance of nested PCR with expert microscopy as a means of detecting Plasmodium parasites during active malaria surveillance in western Thailand. Methods The study was performed from May 2000 to April 2002 in the village of Kong Mong Tha, located in western Thailand. Plasmodium vivax (PV) and Plasmodium falciparum (PF) are the predominant parasite species in this village, followed by Plasmodium malariae (PM) and Plasmodium ovale (PO). Each month, fingerprick blood samples were taken from each participating individual and used to prepare thick and thin blood films and for PCR analysis. Results PCR was sensitive (96%) and specific (98%) for malaria at parasite densities ≥ 500/μl; however, only 18% (47/269) of P. falciparum - and 5% (20/390) of P. vivax -positive films had parasite densities this high. Performance of PCR decreased markedly at parasite densities <500/μl, with sensitivity of only 20% for P. falciparum and 24% for P. vivax at densities <100 parasites/μl. Conclusion Although PCR performance appeared poor when compared to microscopy, data indicated that the discrepancy between the two methods resulted from poor performance of microscopy at low parasite densities rather than poor performance of PCR. These data are not unusual when the diagnostic method being evaluated is more sensitive than the reference method. PCR appears to be a useful method for detecting Plasmodium parasites during active malaria surveillance in Thailand. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 5 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Miller Robert Zollner Gabriela Rachapaew Nattawan Kengluecha Ampornpan Tippayachai Bousaraporn Maneechai Nongnuj Promstaporm Sommai Sattabongkot Jetsumon Coleman Russell E Vaughan Jefferson A Thimasarn Krongtong Khuntirat Benjawan Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Objective The main objective of this study was to compare the performance of nested PCR with expert microscopy as a means of detecting Plasmodium parasites during active malaria surveillance in western Thailand. Methods The study was performed from May 2000 to April 2002 in the village of Kong Mong Tha, located in western Thailand. Plasmodium vivax (PV) and Plasmodium falciparum (PF) are the predominant parasite species in this village, followed by Plasmodium malariae (PM) and Plasmodium ovale (PO). Each month, fingerprick blood samples were taken from each participating individual and used to prepare thick and thin blood films and for PCR analysis. Results PCR was sensitive (96%) and specific (98%) for malaria at parasite densities ≥ 500/μl; however, only 18% (47/269) of P. falciparum - and 5% (20/390) of P. vivax -positive films had parasite densities this high. Performance of PCR decreased markedly at parasite densities <500/μl, with sensitivity of only 20% for P. falciparum and 24% for P. vivax at densities <100 parasites/μl. Conclusion Although PCR performance appeared poor when compared to microscopy, data indicated that the discrepancy between the two methods resulted from poor performance of microscopy at low parasite densities rather than poor performance of PCR. These data are not unusual when the diagnostic method being evaluated is more sensitive than the reference method. PCR appears to be a useful method for detecting Plasmodium parasites during active malaria surveillance in Thailand. |
format |
Article in Journal/Newspaper |
author |
Miller Robert Zollner Gabriela Rachapaew Nattawan Kengluecha Ampornpan Tippayachai Bousaraporn Maneechai Nongnuj Promstaporm Sommai Sattabongkot Jetsumon Coleman Russell E Vaughan Jefferson A Thimasarn Krongtong Khuntirat Benjawan |
author_facet |
Miller Robert Zollner Gabriela Rachapaew Nattawan Kengluecha Ampornpan Tippayachai Bousaraporn Maneechai Nongnuj Promstaporm Sommai Sattabongkot Jetsumon Coleman Russell E Vaughan Jefferson A Thimasarn Krongtong Khuntirat Benjawan |
author_sort |
Miller Robert |
title |
Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand |
title_short |
Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand |
title_full |
Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand |
title_fullStr |
Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand |
title_full_unstemmed |
Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand |
title_sort |
comparison of pcr and microscopy for the detection of asymptomatic malaria in a plasmodium falciparum/vivax endemic area in thailand |
publisher |
BMC |
publishDate |
2006 |
url |
https://doi.org/10.1186/1475-2875-5-121 https://doaj.org/article/10cf4d4554f044a79e625936609fade7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 5, Iss 1, p 121 (2006) |
op_relation |
http://www.malariajournal.com/content/5/1/121 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-5-121 1475-2875 https://doaj.org/article/10cf4d4554f044a79e625936609fade7 |
op_doi |
https://doi.org/10.1186/1475-2875-5-121 |
container_title |
Malaria Journal |
container_volume |
5 |
container_issue |
1 |
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1766341520734027776 |