Hindgut microbiota in laboratory-reared and wild Triatoma infestans.
Triatomine vectors transmit Trypanosoma cruzi, the etiological agent of Chagas disease in humans. Transmission to humans typically occurs when contaminated triatomine feces come in contact with the bite site or mucosal membranes. In the Southern Cone of South America, where the highest burden of dis...
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ftdoajarticles:oai:doaj.org/article:109642566c51445dadaa75cdbedf766b 2023-05-15T15:16:26+02:00 Hindgut microbiota in laboratory-reared and wild Triatoma infestans. Andreea Waltmann Alexandra C Willcox Sujata Balasubramanian Katty Borrini Mayori Sandra Mendoza Guerrero Renzo S Salazar Sanchez Jeffrey Roach Carlos Condori Pino Robert H Gilman Caryn Bern Jonathan J Juliano Michael Z Levy Steven R Meshnick Natalie M Bowman 2019-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007383 https://doaj.org/article/109642566c51445dadaa75cdbedf766b EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007383 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007383 https://doaj.org/article/109642566c51445dadaa75cdbedf766b PLoS Neglected Tropical Diseases, Vol 13, Iss 5, p e0007383 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007383 2022-12-31T06:00:46Z Triatomine vectors transmit Trypanosoma cruzi, the etiological agent of Chagas disease in humans. Transmission to humans typically occurs when contaminated triatomine feces come in contact with the bite site or mucosal membranes. In the Southern Cone of South America, where the highest burden of disease exists, Triatoma infestans is the principal vector for T. cruzi. Recent studies of other vector-borne illnesses have shown that arthropod microbiota influences the ability of infectious agents to colonize the insect vector and transmit to the human host. This has garnered attention as a potential control strategy against T. cruzi, as vector control is the main tool of Chagas disease prevention. Here we characterized the microbiota in T. infestans feces of both wild-caught and laboratory-reared insects and examined the relationship between microbial composition and T. cruzi infection using highly sensitive high-throughput sequencing technology to sequence the V3-V4 region of the 16S ribosomal RNA gene on the MiSeq Illumina platform. We collected 59 wild (9 with T. cruzi infection) and 10 lab-reared T. infestans (4 with T. cruzi infection) from the endemic area of Arequipa, Perú. Wild T. infestans had greater hindgut bacterial diversity than laboratory-reared bugs. Microbiota of lab insects comprised a subset of those identified in their wild counterparts, with 96 of the total 124 genera also observed in laboratory-reared insects. Among wild insects, variation in bacterial composition was observed, but time and location of collection and development stage did not explain this variation. T. cruzi infection in lab insects did not affect α- or β-diversity; however, we did find that the β-diversity of wild insects differed if they were infected with T. cruzi and identified 10 specific taxa that had significantly different relative abundances in infected vs. uninfected wild T. infestans (Bosea, Mesorhizobium, Dietzia, and Cupriavidus were underrepresented in infected bugs; Sporosarcina, an unclassified genus of ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 5 e0007383 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Andreea Waltmann Alexandra C Willcox Sujata Balasubramanian Katty Borrini Mayori Sandra Mendoza Guerrero Renzo S Salazar Sanchez Jeffrey Roach Carlos Condori Pino Robert H Gilman Caryn Bern Jonathan J Juliano Michael Z Levy Steven R Meshnick Natalie M Bowman Hindgut microbiota in laboratory-reared and wild Triatoma infestans. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Triatomine vectors transmit Trypanosoma cruzi, the etiological agent of Chagas disease in humans. Transmission to humans typically occurs when contaminated triatomine feces come in contact with the bite site or mucosal membranes. In the Southern Cone of South America, where the highest burden of disease exists, Triatoma infestans is the principal vector for T. cruzi. Recent studies of other vector-borne illnesses have shown that arthropod microbiota influences the ability of infectious agents to colonize the insect vector and transmit to the human host. This has garnered attention as a potential control strategy against T. cruzi, as vector control is the main tool of Chagas disease prevention. Here we characterized the microbiota in T. infestans feces of both wild-caught and laboratory-reared insects and examined the relationship between microbial composition and T. cruzi infection using highly sensitive high-throughput sequencing technology to sequence the V3-V4 region of the 16S ribosomal RNA gene on the MiSeq Illumina platform. We collected 59 wild (9 with T. cruzi infection) and 10 lab-reared T. infestans (4 with T. cruzi infection) from the endemic area of Arequipa, Perú. Wild T. infestans had greater hindgut bacterial diversity than laboratory-reared bugs. Microbiota of lab insects comprised a subset of those identified in their wild counterparts, with 96 of the total 124 genera also observed in laboratory-reared insects. Among wild insects, variation in bacterial composition was observed, but time and location of collection and development stage did not explain this variation. T. cruzi infection in lab insects did not affect α- or β-diversity; however, we did find that the β-diversity of wild insects differed if they were infected with T. cruzi and identified 10 specific taxa that had significantly different relative abundances in infected vs. uninfected wild T. infestans (Bosea, Mesorhizobium, Dietzia, and Cupriavidus were underrepresented in infected bugs; Sporosarcina, an unclassified genus of ... |
format |
Article in Journal/Newspaper |
author |
Andreea Waltmann Alexandra C Willcox Sujata Balasubramanian Katty Borrini Mayori Sandra Mendoza Guerrero Renzo S Salazar Sanchez Jeffrey Roach Carlos Condori Pino Robert H Gilman Caryn Bern Jonathan J Juliano Michael Z Levy Steven R Meshnick Natalie M Bowman |
author_facet |
Andreea Waltmann Alexandra C Willcox Sujata Balasubramanian Katty Borrini Mayori Sandra Mendoza Guerrero Renzo S Salazar Sanchez Jeffrey Roach Carlos Condori Pino Robert H Gilman Caryn Bern Jonathan J Juliano Michael Z Levy Steven R Meshnick Natalie M Bowman |
author_sort |
Andreea Waltmann |
title |
Hindgut microbiota in laboratory-reared and wild Triatoma infestans. |
title_short |
Hindgut microbiota in laboratory-reared and wild Triatoma infestans. |
title_full |
Hindgut microbiota in laboratory-reared and wild Triatoma infestans. |
title_fullStr |
Hindgut microbiota in laboratory-reared and wild Triatoma infestans. |
title_full_unstemmed |
Hindgut microbiota in laboratory-reared and wild Triatoma infestans. |
title_sort |
hindgut microbiota in laboratory-reared and wild triatoma infestans. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007383 https://doaj.org/article/109642566c51445dadaa75cdbedf766b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 5, p e0007383 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007383 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007383 https://doaj.org/article/109642566c51445dadaa75cdbedf766b |
op_doi |
https://doi.org/10.1371/journal.pntd.0007383 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
5 |
container_start_page |
e0007383 |
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1766346734684864512 |