Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes
Boreal lakes and ponds produce two-thirds of the total natural methane emissions above the latitude of 50° North. These lake emissions are regulated by methanotrophs which can oxidize up to 99% of the methane produced in the sediments and the water column. Despite their importance, the diversity and...
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Frontiers Media S.A.
2021
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ftdoajarticles:oai:doaj.org/article:108b6d9be57e41fe9539dce506dc2320 2023-05-15T18:28:33+02:00 Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes Gaëtan Martin Antti J. Rissanen Sarahi L. Garcia Maliheh Mehrshad Moritz Buck Sari Peura 2021-08-01T00:00:00Z https://doi.org/10.3389/fmicb.2021.669937 https://doaj.org/article/108b6d9be57e41fe9539dce506dc2320 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmicb.2021.669937/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2021.669937 https://doaj.org/article/108b6d9be57e41fe9539dce506dc2320 Frontiers in Microbiology, Vol 12 (2021) methanotroph greenhouse gas metagenomics thaw ponds microbial diversity lakes Microbiology QR1-502 article 2021 ftdoajarticles https://doi.org/10.3389/fmicb.2021.669937 2022-12-31T09:52:50Z Boreal lakes and ponds produce two-thirds of the total natural methane emissions above the latitude of 50° North. These lake emissions are regulated by methanotrophs which can oxidize up to 99% of the methane produced in the sediments and the water column. Despite their importance, the diversity and distribution of the methanotrophs in lakes are still poorly understood. Here, we used shotgun metagenomic data to explore the diversity and distribution of methanotrophs in 40 oxygen-stratified water bodies in boreal and subarctic areas in Europe and North America. In our data, gammaproteobacterial methanotrophs (order Methylococcales) generally dominated the methanotrophic communities throughout the water columns. A recently discovered lineage of Methylococcales, Candidatus Methylumidiphilus, was present in all the studied water bodies and dominated the methanotrophic community in lakes with a high relative abundance of methanotrophs. Alphaproteobacterial methanotrophs were the second most abundant group of methanotrophs. In the top layer of the lakes, characterized by low CH4 concentration, their abundance could surpass that of the gammaproteobacterial methanotrophs. These results support the theory that the alphaproteobacterial methanotrophs have a high affinity for CH4 and can be considered stress-tolerant strategists. In contrast, the gammaproteobacterial methanotrophs are competitive strategists. In addition, relative abundances of anaerobic methanotrophs, Candidatus Methanoperedenaceae and Candidatus Methylomirabilis, were strongly correlated, suggesting possible co-metabolism. Our data also suggest that these anaerobic methanotrophs could be active even in the oxic layers. In non-metric multidimensional scaling, alpha- and gammaproteobacterial methanotrophs formed separate clusters based on their abundances in the samples, except for the gammaproteobacterial Candidatus Methylumidiphilus, which was separated from these two clusters. This may reflect similarities in the niche and environmental requirements of ... Article in Journal/Newspaper Subarctic Directory of Open Access Journals: DOAJ Articles Frontiers in Microbiology 12 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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methanotroph greenhouse gas metagenomics thaw ponds microbial diversity lakes Microbiology QR1-502 |
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methanotroph greenhouse gas metagenomics thaw ponds microbial diversity lakes Microbiology QR1-502 Gaëtan Martin Antti J. Rissanen Sarahi L. Garcia Maliheh Mehrshad Moritz Buck Sari Peura Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes |
topic_facet |
methanotroph greenhouse gas metagenomics thaw ponds microbial diversity lakes Microbiology QR1-502 |
description |
Boreal lakes and ponds produce two-thirds of the total natural methane emissions above the latitude of 50° North. These lake emissions are regulated by methanotrophs which can oxidize up to 99% of the methane produced in the sediments and the water column. Despite their importance, the diversity and distribution of the methanotrophs in lakes are still poorly understood. Here, we used shotgun metagenomic data to explore the diversity and distribution of methanotrophs in 40 oxygen-stratified water bodies in boreal and subarctic areas in Europe and North America. In our data, gammaproteobacterial methanotrophs (order Methylococcales) generally dominated the methanotrophic communities throughout the water columns. A recently discovered lineage of Methylococcales, Candidatus Methylumidiphilus, was present in all the studied water bodies and dominated the methanotrophic community in lakes with a high relative abundance of methanotrophs. Alphaproteobacterial methanotrophs were the second most abundant group of methanotrophs. In the top layer of the lakes, characterized by low CH4 concentration, their abundance could surpass that of the gammaproteobacterial methanotrophs. These results support the theory that the alphaproteobacterial methanotrophs have a high affinity for CH4 and can be considered stress-tolerant strategists. In contrast, the gammaproteobacterial methanotrophs are competitive strategists. In addition, relative abundances of anaerobic methanotrophs, Candidatus Methanoperedenaceae and Candidatus Methylomirabilis, were strongly correlated, suggesting possible co-metabolism. Our data also suggest that these anaerobic methanotrophs could be active even in the oxic layers. In non-metric multidimensional scaling, alpha- and gammaproteobacterial methanotrophs formed separate clusters based on their abundances in the samples, except for the gammaproteobacterial Candidatus Methylumidiphilus, which was separated from these two clusters. This may reflect similarities in the niche and environmental requirements of ... |
format |
Article in Journal/Newspaper |
author |
Gaëtan Martin Antti J. Rissanen Sarahi L. Garcia Maliheh Mehrshad Moritz Buck Sari Peura |
author_facet |
Gaëtan Martin Antti J. Rissanen Sarahi L. Garcia Maliheh Mehrshad Moritz Buck Sari Peura |
author_sort |
Gaëtan Martin |
title |
Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes |
title_short |
Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes |
title_full |
Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes |
title_fullStr |
Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes |
title_full_unstemmed |
Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes |
title_sort |
candidatus methylumidiphilus drives peaks in methanotrophic relative abundance in stratified lakes and ponds across northern landscapes |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fmicb.2021.669937 https://doaj.org/article/108b6d9be57e41fe9539dce506dc2320 |
genre |
Subarctic |
genre_facet |
Subarctic |
op_source |
Frontiers in Microbiology, Vol 12 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmicb.2021.669937/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2021.669937 https://doaj.org/article/108b6d9be57e41fe9539dce506dc2320 |
op_doi |
https://doi.org/10.3389/fmicb.2021.669937 |
container_title |
Frontiers in Microbiology |
container_volume |
12 |
_version_ |
1766211069424959488 |