Assessment of hepatitis B virus genotype D and interleukin-10, interferon gamma, and tumor necrosis factor-α in fulminant hepatic failure
Background: Genotypically, hepatitis B virus (HBV) is divided into 8 groups, A–H. The interaction between HBV replication and immune responses against HBV infection plays an important role in the pathogenesis of virus infection. Aims and Objectives: In an attempt to elucidate the role of immunomodul...
Published in: | CHRISMED Journal of Health and Research |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2019
|
Subjects: | |
Online Access: | https://doi.org/10.4103/cjhr.cjhr_131_18 https://doaj.org/article/10607a0876904b5a86ba2fcb0cdf63d1 |
id |
ftdoajarticles:oai:doaj.org/article:10607a0876904b5a86ba2fcb0cdf63d1 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:10607a0876904b5a86ba2fcb0cdf63d1 2023-05-15T18:13:23+02:00 Assessment of hepatitis B virus genotype D and interleukin-10, interferon gamma, and tumor necrosis factor-α in fulminant hepatic failure Meher Rizvi Hiba Sami Mohd Azam Indu Shukla Haris M Khan M R Ajmal 2019-01-01T00:00:00Z https://doi.org/10.4103/cjhr.cjhr_131_18 https://doaj.org/article/10607a0876904b5a86ba2fcb0cdf63d1 EN eng Wolters Kluwer Medknow Publications http://www.cjhr.org/article.asp?issn=2348-3334;year=2019;volume=6;issue=3;spage=162;epage=166;aulast=Rizvi https://doaj.org/toc/2348-3334 https://doaj.org/toc/2348-506X 2348-3334 2348-506X doi:10.4103/cjhr.cjhr_131_18 https://doaj.org/article/10607a0876904b5a86ba2fcb0cdf63d1 CHRISMED Journal of Health and Research, Vol 6, Iss 3, Pp 162-166 (2019) Fulminant hepatitis genotype D Hepatitis B virus Medicine R Nursing RT1-120 article 2019 ftdoajarticles https://doi.org/10.4103/cjhr.cjhr_131_18 2022-12-31T01:51:53Z Background: Genotypically, hepatitis B virus (HBV) is divided into 8 groups, A–H. The interaction between HBV replication and immune responses against HBV infection plays an important role in the pathogenesis of virus infection. Aims and Objectives: In an attempt to elucidate the role of immunomodulatory, pro-inflammatory, and anti-inflammatory mediators in the pathogenesis of HBV genotype D fulminant hepatitis (FH), we assessed the role of interferon gamma (IFN-γ), tumor necrosis factor-α (TNF-α), and interleukin-10 (IL-10) in such patients. Subjects and Methods: Two hundred and seventy-six cases of acute hepatitis among which 37 cases of fulminant hepatic failure were screened. A genotyping system based on polymerase chain reaction using type-specific primers was used in this study (Sami et al. 2013). TNF-α, IL-10 levels, and IFN-γ serum level were measured by ELISA. Results: Genotype D was detected in 10 cases (27.02%) out of 37 FH patients. Mean IL-10, IFN-γ, and TNF-α levels in FH patients were significantly higher than the healthy controls at 127.6 ± 73.45 pg/ml (P < 0.001), 10.6778 ± 5.23 pg/ml (P < 0.0001), and 62.9000 ± 21.67 pg/ml (P < 0.001), respectively. IFN-γ and IL-10 levels were much higher in cases of hepatitis B e-antigen (HBeAg) seronegative individuals, mean levels being 20.77 pg/ml and 36.53 pg/ml, respectively. Using multiple linear regression analysis, the presence of HBeAg was inversely associated with TNF-α. Conclusion: This study clearly highlights the excessive dysregulated response of the three cytokines, more so of IL-10 which appears pivotal in the pathogenesis of FH. Interventions leading to suppression of IL-10 levels may be beneficial in improving the patient outcome. Article in Journal/Newspaper sami Directory of Open Access Journals: DOAJ Articles CHRISMED Journal of Health and Research 6 3 162 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Fulminant hepatitis genotype D Hepatitis B virus Medicine R Nursing RT1-120 |
spellingShingle |
Fulminant hepatitis genotype D Hepatitis B virus Medicine R Nursing RT1-120 Meher Rizvi Hiba Sami Mohd Azam Indu Shukla Haris M Khan M R Ajmal Assessment of hepatitis B virus genotype D and interleukin-10, interferon gamma, and tumor necrosis factor-α in fulminant hepatic failure |
topic_facet |
Fulminant hepatitis genotype D Hepatitis B virus Medicine R Nursing RT1-120 |
description |
Background: Genotypically, hepatitis B virus (HBV) is divided into 8 groups, A–H. The interaction between HBV replication and immune responses against HBV infection plays an important role in the pathogenesis of virus infection. Aims and Objectives: In an attempt to elucidate the role of immunomodulatory, pro-inflammatory, and anti-inflammatory mediators in the pathogenesis of HBV genotype D fulminant hepatitis (FH), we assessed the role of interferon gamma (IFN-γ), tumor necrosis factor-α (TNF-α), and interleukin-10 (IL-10) in such patients. Subjects and Methods: Two hundred and seventy-six cases of acute hepatitis among which 37 cases of fulminant hepatic failure were screened. A genotyping system based on polymerase chain reaction using type-specific primers was used in this study (Sami et al. 2013). TNF-α, IL-10 levels, and IFN-γ serum level were measured by ELISA. Results: Genotype D was detected in 10 cases (27.02%) out of 37 FH patients. Mean IL-10, IFN-γ, and TNF-α levels in FH patients were significantly higher than the healthy controls at 127.6 ± 73.45 pg/ml (P < 0.001), 10.6778 ± 5.23 pg/ml (P < 0.0001), and 62.9000 ± 21.67 pg/ml (P < 0.001), respectively. IFN-γ and IL-10 levels were much higher in cases of hepatitis B e-antigen (HBeAg) seronegative individuals, mean levels being 20.77 pg/ml and 36.53 pg/ml, respectively. Using multiple linear regression analysis, the presence of HBeAg was inversely associated with TNF-α. Conclusion: This study clearly highlights the excessive dysregulated response of the three cytokines, more so of IL-10 which appears pivotal in the pathogenesis of FH. Interventions leading to suppression of IL-10 levels may be beneficial in improving the patient outcome. |
format |
Article in Journal/Newspaper |
author |
Meher Rizvi Hiba Sami Mohd Azam Indu Shukla Haris M Khan M R Ajmal |
author_facet |
Meher Rizvi Hiba Sami Mohd Azam Indu Shukla Haris M Khan M R Ajmal |
author_sort |
Meher Rizvi |
title |
Assessment of hepatitis B virus genotype D and interleukin-10, interferon gamma, and tumor necrosis factor-α in fulminant hepatic failure |
title_short |
Assessment of hepatitis B virus genotype D and interleukin-10, interferon gamma, and tumor necrosis factor-α in fulminant hepatic failure |
title_full |
Assessment of hepatitis B virus genotype D and interleukin-10, interferon gamma, and tumor necrosis factor-α in fulminant hepatic failure |
title_fullStr |
Assessment of hepatitis B virus genotype D and interleukin-10, interferon gamma, and tumor necrosis factor-α in fulminant hepatic failure |
title_full_unstemmed |
Assessment of hepatitis B virus genotype D and interleukin-10, interferon gamma, and tumor necrosis factor-α in fulminant hepatic failure |
title_sort |
assessment of hepatitis b virus genotype d and interleukin-10, interferon gamma, and tumor necrosis factor-α in fulminant hepatic failure |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/cjhr.cjhr_131_18 https://doaj.org/article/10607a0876904b5a86ba2fcb0cdf63d1 |
genre |
sami |
genre_facet |
sami |
op_source |
CHRISMED Journal of Health and Research, Vol 6, Iss 3, Pp 162-166 (2019) |
op_relation |
http://www.cjhr.org/article.asp?issn=2348-3334;year=2019;volume=6;issue=3;spage=162;epage=166;aulast=Rizvi https://doaj.org/toc/2348-3334 https://doaj.org/toc/2348-506X 2348-3334 2348-506X doi:10.4103/cjhr.cjhr_131_18 https://doaj.org/article/10607a0876904b5a86ba2fcb0cdf63d1 |
op_doi |
https://doi.org/10.4103/cjhr.cjhr_131_18 |
container_title |
CHRISMED Journal of Health and Research |
container_volume |
6 |
container_issue |
3 |
container_start_page |
162 |
_version_ |
1766185893906874368 |