Medium-term cryopreservation of rabies virus samples
Introduction The cryopreservation of rabies virus has been described in detail in the literature. To date, little information is available on the use of cryoprotective agents for cold preservation of this virus, and the available data focus only on short-term virus preservation. In this study, we in...
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ftdoajarticles:oai:doaj.org/article:105c6f3b626e4fee95ffe25a0ed71c2e 2023-05-15T15:09:36+02:00 Medium-term cryopreservation of rabies virus samples Tereza D'avila de Freitas Aguiar Maria Fatima da Silva Teixeira Edmara Chaves Costa Allan Bezerra Vitaliano Carlos Henrique de Andrade Teles Igor Ciriaco Barroso Ronaldo Pereira Dias Nelio Batista de Moraes 2013-12-01T00:00:00Z https://doi.org/10.1590/0037-8682-0135-2013 https://doaj.org/article/105c6f3b626e4fee95ffe25a0ed71c2e EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000600678&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0135-2013 https://doaj.org/article/105c6f3b626e4fee95ffe25a0ed71c2e Revista da Sociedade Brasileira de Medicina Tropical, Vol 46, Iss 6, Pp 678-683 (2013) Cryopreservation Rabies virus Medium-term Arctic medicine. Tropical medicine RC955-962 article 2013 ftdoajarticles https://doi.org/10.1590/0037-8682-0135-2013 2022-12-31T02:57:59Z Introduction The cryopreservation of rabies virus has been described in detail in the literature. To date, little information is available on the use of cryoprotective agents for cold preservation of this virus, and the available data focus only on short-term virus preservation. In this study, we investigated the medium-term cryopreservation of samples of rabies virus using different cryopreservation protocols. Methods The cryopreservation protocols for the rabies virus samples were performed at -20°C and were divided according to the variables of time and cryoprotectant type used. The laboratory tests (intracerebral inoculation of mice, viral titration and direct immunofluorescence) were performed at regular intervals (360 and 720 days) to assess the viability of the viral samples according to the different preservation techniques used. Results After 1 year of cryopreservation, the fluorescence intensity of intracellular corpuscles of the rabies virus and the median survival time of the mice differed between the positive controls and the treatments with the cryoprotectants. After 2 years, most of the samples subjected to the cryopreservation protocols (including the controls) did not produce fluorescence. However, the virus samples exposed to the cryoprotectant sucrose (68% solution) responded positively in the direct immunofluorescence assay and in the intracerebral inoculation of the mice. Conclusions Medium-term cryopreservation of the rabies virus inactivates the viral sample. However, the cryoprotectant agent sucrose (68%) produces a preservative effect in cryopreserved rabies virus samples. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 46 6 678 683 |
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English |
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Cryopreservation Rabies virus Medium-term Arctic medicine. Tropical medicine RC955-962 |
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Cryopreservation Rabies virus Medium-term Arctic medicine. Tropical medicine RC955-962 Tereza D'avila de Freitas Aguiar Maria Fatima da Silva Teixeira Edmara Chaves Costa Allan Bezerra Vitaliano Carlos Henrique de Andrade Teles Igor Ciriaco Barroso Ronaldo Pereira Dias Nelio Batista de Moraes Medium-term cryopreservation of rabies virus samples |
topic_facet |
Cryopreservation Rabies virus Medium-term Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction The cryopreservation of rabies virus has been described in detail in the literature. To date, little information is available on the use of cryoprotective agents for cold preservation of this virus, and the available data focus only on short-term virus preservation. In this study, we investigated the medium-term cryopreservation of samples of rabies virus using different cryopreservation protocols. Methods The cryopreservation protocols for the rabies virus samples were performed at -20°C and were divided according to the variables of time and cryoprotectant type used. The laboratory tests (intracerebral inoculation of mice, viral titration and direct immunofluorescence) were performed at regular intervals (360 and 720 days) to assess the viability of the viral samples according to the different preservation techniques used. Results After 1 year of cryopreservation, the fluorescence intensity of intracellular corpuscles of the rabies virus and the median survival time of the mice differed between the positive controls and the treatments with the cryoprotectants. After 2 years, most of the samples subjected to the cryopreservation protocols (including the controls) did not produce fluorescence. However, the virus samples exposed to the cryoprotectant sucrose (68% solution) responded positively in the direct immunofluorescence assay and in the intracerebral inoculation of the mice. Conclusions Medium-term cryopreservation of the rabies virus inactivates the viral sample. However, the cryoprotectant agent sucrose (68%) produces a preservative effect in cryopreserved rabies virus samples. |
format |
Article in Journal/Newspaper |
author |
Tereza D'avila de Freitas Aguiar Maria Fatima da Silva Teixeira Edmara Chaves Costa Allan Bezerra Vitaliano Carlos Henrique de Andrade Teles Igor Ciriaco Barroso Ronaldo Pereira Dias Nelio Batista de Moraes |
author_facet |
Tereza D'avila de Freitas Aguiar Maria Fatima da Silva Teixeira Edmara Chaves Costa Allan Bezerra Vitaliano Carlos Henrique de Andrade Teles Igor Ciriaco Barroso Ronaldo Pereira Dias Nelio Batista de Moraes |
author_sort |
Tereza D'avila de Freitas Aguiar |
title |
Medium-term cryopreservation of rabies virus samples |
title_short |
Medium-term cryopreservation of rabies virus samples |
title_full |
Medium-term cryopreservation of rabies virus samples |
title_fullStr |
Medium-term cryopreservation of rabies virus samples |
title_full_unstemmed |
Medium-term cryopreservation of rabies virus samples |
title_sort |
medium-term cryopreservation of rabies virus samples |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2013 |
url |
https://doi.org/10.1590/0037-8682-0135-2013 https://doaj.org/article/105c6f3b626e4fee95ffe25a0ed71c2e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 46, Iss 6, Pp 678-683 (2013) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000600678&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0135-2013 https://doaj.org/article/105c6f3b626e4fee95ffe25a0ed71c2e |
op_doi |
https://doi.org/10.1590/0037-8682-0135-2013 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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46 |
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