Comparación entre los métodos de inmunocromatografía e inmunoensayo enzimático (ELISA) en el diagnóstico del dengue

• Main Authors: Valero Nereida, Montiel Milagros, Arias Julia, Fuentes Belkis, Mavarez Alibeth, Nava Lesmy, Hernández José
• Format: Article in Journal/Newspaper
• Language: English; Spanish
• Published: Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales 2006
• Subjects: Anticuerpos; dengue; inmunoanálisis enzimático; inmunocromatografía; Antibodies; enzymatic immune-analysis; immune-chromatography; Arctic medicine. Tropical medicine; RC955-962; Public aspects of medicine; RA1-1270; Arctic
• Online Access: https://doaj.org/article/103b42b89c7246d6b4f1ad010276dde1

Abstract: The search for techniques for the diagnostic confirmation of Dengue (IgM and IgG) and recent commercialization of such serums requires of the study of their sensibility, specificity, reproductibility, speed and costs. For the purpose of comparing two serological techniques for the diagnosis of this infection, 184 serum samples of patients with confirmed diagnosis of Dengue and 60 with Rubella, were analyzed by immune-chromatographic (IC) and enzyme linked immune-absorbent tests (ELISA) by determination of anti-dengue antibody IgM and IgG. In the IC and ELISA techniques levels of 0,70 and 0,79 in nosologic sensibility were obtained, respectively in the determination of antibody IgM; while the sensibility of ELISA IgG resulted significantly elevated (p<0,0001) with respect to IC (0,92 vs 0,65). Both techniques reached reproduction levels of 98% and a specificity of 100%. These results suggest that ELISA is best for the detection of primary and secondary infections, while IC was the fastest assay and applicable in outbreaks and epidemics; however, it was less sensitive for the diagnosis of secondary infections for dengue virus and consequently less adequate for the screening of dengue hemorrhagic fever and dengue shock syndrome (DHF/DSS) in endemic areas. Resumen: La búsqueda de técnicas para la confirmación diagnóstica de Dengue y su reciente comercialización requieren su estudio en cuanto a sensibilidad, especificidad, reproducibilidad, rapidez y costo. Con el objetivo de comparar dos técnicas serológicas para el diagnóstico de esta infección, se analizaron 184 sueros provenientes de pacientes con diagnóstico confirmatorio de Dengue y 60 de Rubéola para la evaluación de la especificidad. Fueron procesados por el método de Inmunocromatografía (IC) e Inmunoensayo Enzimático (ELISA) para la determinación de anticuerpos IgM e IgG antidengue. Para IC y ELISA se obtuvo un 0,70 y 0,79 respectivamente, de sensibilidad nosológica en la determinación de anticuerpos IgM; en la determinación de IgG la ...