Immunologic activation of human syncytiotrophoblast by Plasmodium falciparum
Abstract Background Malaria during pregnancy is characterized by the sequestration of malaria-infected red blood cells (iRBC) in the intervillous spaces of the placenta, often accompanied by the infiltration of maternal mononuclear cells, causing substantial maternal and foetal/infant morbidity. The...
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ftdoajarticles:oai:doaj.org/article:101c77dbff9b485b9bf0674106a3b8f6 2023-05-15T15:11:03+02:00 Immunologic activation of human syncytiotrophoblast by Plasmodium falciparum Peterson David S Lucchi Naomi W Moore Julie M 2008-02-01T00:00:00Z https://doi.org/10.1186/1475-2875-7-42 https://doaj.org/article/101c77dbff9b485b9bf0674106a3b8f6 EN eng BMC http://www.malariajournal.com/content/7/1/42 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-42 1475-2875 https://doaj.org/article/101c77dbff9b485b9bf0674106a3b8f6 Malaria Journal, Vol 7, Iss 1, p 42 (2008) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2008 ftdoajarticles https://doi.org/10.1186/1475-2875-7-42 2022-12-31T08:21:59Z Abstract Background Malaria during pregnancy is characterized by the sequestration of malaria-infected red blood cells (iRBC) in the intervillous spaces of the placenta, often accompanied by the infiltration of maternal mononuclear cells, causing substantial maternal and foetal/infant morbidity. The iRBC bind to receptors expressed by the syncytiotrophoblast (ST). How ST responds to this interaction remains poorly understood. Because it is known that ST is immunoactive and can respond to infectious agents, the consequences of this ST-iRBC interaction should be investigated. Methods An in vitro system was used to assess the biochemical and immunological changes induced in ST by ST-adherent iRBCs. Changes in ST mitogen-activated protein kinase (MAPK) activation were assessed by immunoblotting and mRNA expression levels of selected cytokine and chemokines in primary ST bound by iRBC were determined using real-time, reverse transcription PCR. In addition, secreted cytokine and chemokine proteins were assayed by standard ELISA, and chemotaxis of PBMC was assessed using a two-chamber assay system. Results Following iRBC/ST interaction, ST C-Jun N-terminal kinase 1 (JNK1) was activated and modest increases in the mRNA expression of TGF-β and IL-8/CXCL8 were observed. In addition, this interaction increased secretion of MIF and MIP-1α/CCL3 by ST and induced migration of PBMC towards iRBC-stimulated ST. Conclusion Results from this study provide the first evidence that ST participates in shaping the local immunological milieu and in the recruitment of maternal immune cells to the maternal blood space during placental malaria infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 7 1 42 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Peterson David S Lucchi Naomi W Moore Julie M Immunologic activation of human syncytiotrophoblast by Plasmodium falciparum |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria during pregnancy is characterized by the sequestration of malaria-infected red blood cells (iRBC) in the intervillous spaces of the placenta, often accompanied by the infiltration of maternal mononuclear cells, causing substantial maternal and foetal/infant morbidity. The iRBC bind to receptors expressed by the syncytiotrophoblast (ST). How ST responds to this interaction remains poorly understood. Because it is known that ST is immunoactive and can respond to infectious agents, the consequences of this ST-iRBC interaction should be investigated. Methods An in vitro system was used to assess the biochemical and immunological changes induced in ST by ST-adherent iRBCs. Changes in ST mitogen-activated protein kinase (MAPK) activation were assessed by immunoblotting and mRNA expression levels of selected cytokine and chemokines in primary ST bound by iRBC were determined using real-time, reverse transcription PCR. In addition, secreted cytokine and chemokine proteins were assayed by standard ELISA, and chemotaxis of PBMC was assessed using a two-chamber assay system. Results Following iRBC/ST interaction, ST C-Jun N-terminal kinase 1 (JNK1) was activated and modest increases in the mRNA expression of TGF-β and IL-8/CXCL8 were observed. In addition, this interaction increased secretion of MIF and MIP-1α/CCL3 by ST and induced migration of PBMC towards iRBC-stimulated ST. Conclusion Results from this study provide the first evidence that ST participates in shaping the local immunological milieu and in the recruitment of maternal immune cells to the maternal blood space during placental malaria infection. |
format |
Article in Journal/Newspaper |
author |
Peterson David S Lucchi Naomi W Moore Julie M |
author_facet |
Peterson David S Lucchi Naomi W Moore Julie M |
author_sort |
Peterson David S |
title |
Immunologic activation of human syncytiotrophoblast by Plasmodium falciparum |
title_short |
Immunologic activation of human syncytiotrophoblast by Plasmodium falciparum |
title_full |
Immunologic activation of human syncytiotrophoblast by Plasmodium falciparum |
title_fullStr |
Immunologic activation of human syncytiotrophoblast by Plasmodium falciparum |
title_full_unstemmed |
Immunologic activation of human syncytiotrophoblast by Plasmodium falciparum |
title_sort |
immunologic activation of human syncytiotrophoblast by plasmodium falciparum |
publisher |
BMC |
publishDate |
2008 |
url |
https://doi.org/10.1186/1475-2875-7-42 https://doaj.org/article/101c77dbff9b485b9bf0674106a3b8f6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 7, Iss 1, p 42 (2008) |
op_relation |
http://www.malariajournal.com/content/7/1/42 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-42 1475-2875 https://doaj.org/article/101c77dbff9b485b9bf0674106a3b8f6 |
op_doi |
https://doi.org/10.1186/1475-2875-7-42 |
container_title |
Malaria Journal |
container_volume |
7 |
container_issue |
1 |
container_start_page |
42 |
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1766341967952740352 |