Modeling Powassan virus infection in Peromyscus leucopus, a natural host.
The tick-borne flavivirus, Powassan virus (POWV) causes life-threatening encephalitis in humans in North America and Europe. POWV is transmitted by ixodid tick vectors that feed on small to medium-sized mammals, such as Peromyscus leucopus mice, which may serve as either reservoir, bridge or amplifi...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2017
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0005346 https://doaj.org/article/100965001b3848b693cc8a3235b43362 |
id |
ftdoajarticles:oai:doaj.org/article:100965001b3848b693cc8a3235b43362 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:100965001b3848b693cc8a3235b43362 2023-05-15T15:08:53+02:00 Modeling Powassan virus infection in Peromyscus leucopus, a natural host. Luwanika Mlera Kimberly Meade-White Greg Saturday Dana Scott Marshall E Bloom 2017-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005346 https://doaj.org/article/100965001b3848b693cc8a3235b43362 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5302833?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005346 https://doaj.org/article/100965001b3848b693cc8a3235b43362 PLoS Neglected Tropical Diseases, Vol 11, Iss 1, p e0005346 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005346 2022-12-31T04:12:31Z The tick-borne flavivirus, Powassan virus (POWV) causes life-threatening encephalitis in humans in North America and Europe. POWV is transmitted by ixodid tick vectors that feed on small to medium-sized mammals, such as Peromyscus leucopus mice, which may serve as either reservoir, bridge or amplification hosts. Intraperitoneal and intracranial inoculation of 4-week old Peromyscus leucopus mice with 103 PFU of POWV did not result in overt clinical signs of disease. However, following intracranial inoculation, infected mice seroconverted to POWV and histopathological examinations revealed that the mice uniformly developed mild lymphocytic perivascular cuffing and microgliosis in the brain and spinal cord from 5 to 15 days post infection (dpi), suggesting an early inflammatory response. In contrast, intracranial inoculation of 4-week old C57BL/6 and BALB/c mice was lethal by 5 dpi. Intraperitoneal inoculation was lethal in BALB/c mice, but 40% (2/5) of C57BL/6 mice survived. We concluded that Peromyscus leucopus mice infected i.c. with a lethal dose of POWV support a limited infection, restricted to the central nervous system and mount an antibody response to the virus. However, they fail to develop clinical signs of disease and are able to control the infection. These results suggest the involvement of restriction factors, and the mechanism by which Peromyscus leucopus mice restrict POWV infection remains under study. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 1 e0005346 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Luwanika Mlera Kimberly Meade-White Greg Saturday Dana Scott Marshall E Bloom Modeling Powassan virus infection in Peromyscus leucopus, a natural host. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The tick-borne flavivirus, Powassan virus (POWV) causes life-threatening encephalitis in humans in North America and Europe. POWV is transmitted by ixodid tick vectors that feed on small to medium-sized mammals, such as Peromyscus leucopus mice, which may serve as either reservoir, bridge or amplification hosts. Intraperitoneal and intracranial inoculation of 4-week old Peromyscus leucopus mice with 103 PFU of POWV did not result in overt clinical signs of disease. However, following intracranial inoculation, infected mice seroconverted to POWV and histopathological examinations revealed that the mice uniformly developed mild lymphocytic perivascular cuffing and microgliosis in the brain and spinal cord from 5 to 15 days post infection (dpi), suggesting an early inflammatory response. In contrast, intracranial inoculation of 4-week old C57BL/6 and BALB/c mice was lethal by 5 dpi. Intraperitoneal inoculation was lethal in BALB/c mice, but 40% (2/5) of C57BL/6 mice survived. We concluded that Peromyscus leucopus mice infected i.c. with a lethal dose of POWV support a limited infection, restricted to the central nervous system and mount an antibody response to the virus. However, they fail to develop clinical signs of disease and are able to control the infection. These results suggest the involvement of restriction factors, and the mechanism by which Peromyscus leucopus mice restrict POWV infection remains under study. |
format |
Article in Journal/Newspaper |
author |
Luwanika Mlera Kimberly Meade-White Greg Saturday Dana Scott Marshall E Bloom |
author_facet |
Luwanika Mlera Kimberly Meade-White Greg Saturday Dana Scott Marshall E Bloom |
author_sort |
Luwanika Mlera |
title |
Modeling Powassan virus infection in Peromyscus leucopus, a natural host. |
title_short |
Modeling Powassan virus infection in Peromyscus leucopus, a natural host. |
title_full |
Modeling Powassan virus infection in Peromyscus leucopus, a natural host. |
title_fullStr |
Modeling Powassan virus infection in Peromyscus leucopus, a natural host. |
title_full_unstemmed |
Modeling Powassan virus infection in Peromyscus leucopus, a natural host. |
title_sort |
modeling powassan virus infection in peromyscus leucopus, a natural host. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005346 https://doaj.org/article/100965001b3848b693cc8a3235b43362 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 1, p e0005346 (2017) |
op_relation |
http://europepmc.org/articles/PMC5302833?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005346 https://doaj.org/article/100965001b3848b693cc8a3235b43362 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005346 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
1 |
container_start_page |
e0005346 |
_version_ |
1766340159474761728 |