Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model
INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on th...
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Sociedade Brasileira de Medicina Tropical (SBMT)
2011
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ftdoajarticles:oai:doaj.org/article:0f88a21a0b844cb3b30dc0fd57065e2f 2023-05-15T15:09:32+02:00 Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model César Gómez-Hernández Karine Rezende-Oliveira Gabriel Antônio Nogueira Nascentes Lara Rocha Batista Henrique Borges Kappel José Alejandro Martinez-Ibarra Francisco Trujillo Contreras Eliane Lages-Silva Luis Eduardo Ramírez 2011-12-01T00:00:00Z https://doi.org/10.1590/s0037-86822011005000058 https://doaj.org/article/0f88a21a0b844cb3b30dc0fd57065e2f EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000600007&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/s0037-86822011005000058 https://doaj.org/article/0f88a21a0b844cb3b30dc0fd57065e2f Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 6, Pp 684-690 (2011) Trypanosoma cruzi México Caracterização biológica Caracterização genética Arctic medicine. Tropical medicine RC955-962 article 2011 ftdoajarticles https://doi.org/10.1590/s0037-86822011005000058 2022-12-30T21:27:52Z INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 44 6 684 690 |
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op_collection_id |
ftdoajarticles |
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English |
topic |
Trypanosoma cruzi México Caracterização biológica Caracterização genética Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Trypanosoma cruzi México Caracterização biológica Caracterização genética Arctic medicine. Tropical medicine RC955-962 César Gómez-Hernández Karine Rezende-Oliveira Gabriel Antônio Nogueira Nascentes Lara Rocha Batista Henrique Borges Kappel José Alejandro Martinez-Ibarra Francisco Trujillo Contreras Eliane Lages-Silva Luis Eduardo Ramírez Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model |
topic_facet |
Trypanosoma cruzi México Caracterização biológica Caracterização genética Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI. |
format |
Article in Journal/Newspaper |
author |
César Gómez-Hernández Karine Rezende-Oliveira Gabriel Antônio Nogueira Nascentes Lara Rocha Batista Henrique Borges Kappel José Alejandro Martinez-Ibarra Francisco Trujillo Contreras Eliane Lages-Silva Luis Eduardo Ramírez |
author_facet |
César Gómez-Hernández Karine Rezende-Oliveira Gabriel Antônio Nogueira Nascentes Lara Rocha Batista Henrique Borges Kappel José Alejandro Martinez-Ibarra Francisco Trujillo Contreras Eliane Lages-Silva Luis Eduardo Ramírez |
author_sort |
César Gómez-Hernández |
title |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model |
title_short |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model |
title_full |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model |
title_fullStr |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model |
title_full_unstemmed |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model |
title_sort |
molecular characterization of trypanosoma cruzi mexican strains and their behavior in the mouse experimental model |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2011 |
url |
https://doi.org/10.1590/s0037-86822011005000058 https://doaj.org/article/0f88a21a0b844cb3b30dc0fd57065e2f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 6, Pp 684-690 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000600007&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/s0037-86822011005000058 https://doaj.org/article/0f88a21a0b844cb3b30dc0fd57065e2f |
op_doi |
https://doi.org/10.1590/s0037-86822011005000058 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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44 |
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