Diagnosis of cytomegalovirus infections by qualitative and quantitative PCR in HIV infected patients
A high incidence of cytomegalovirus (CMV) infections is observed in Brazil. These viruses are causatives of significant morbidity and mortality among patients with advanced human immunodeficiency virus (HIV) infection. This work, shows the application of a PCR on determination of CMV load in the buf...
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:0f63fc4e89364277b2b4380dfe39f7f6 2024-09-09T19:26:56+00:00 Diagnosis of cytomegalovirus infections by qualitative and quantitative PCR in HIV infected patients Aldo de Albuquerque CUNHA Lauro Juliano MARIN Victor Hugo AQUINO Luiz Tadeu Moraes FIGUEIREDO 2002-01-01T00:00:00Z https://doaj.org/article/0f63fc4e89364277b2b4380dfe39f7f6 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652002000300003&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/0f63fc4e89364277b2b4380dfe39f7f6 Revista do Instituto de Medicina Tropical de São Paulo, Vol 44, Iss 3, Pp 127-132 (2002) CMV Semiquantitative PCR HIV patients Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2002 ftdoajarticles 2024-08-05T17:49:30Z A high incidence of cytomegalovirus (CMV) infections is observed in Brazil. These viruses are causatives of significant morbidity and mortality among patients with advanced human immunodeficiency virus (HIV) infection. This work, shows the application of a PCR on determination of CMV load in the buffy coat and plasma. We analyzed the samples of 247 HIV infected patients in order to diagnose CMV infection and disease. We developed a semi-quantitative PCR that amplifies part of the glycoprotein B (gB) gene of CMV. The semi-quantitative PCR was carried out only in positive clinical samples in a qualitative PCR confirmed by a nested-PCR. CD4 lymphocyte count, HIV viral load and CMV disease symptom were correlated with CMV load. CMV genome was detected in the buffy coat of 82 of 237 (34.6%) patients, in 10 of these the CMV load was determined varying between 928 and 332 880 viral copies/mug DNA. None of these 237 patients developed any suggestive manifestation of CMV disease. For the other 10 HIV infected patients selected based on the suspicion of CMV disease, CMV genome was detected in only one case. This patient presented a high CMV load, 8 000 000 copies/mug DNA, and developed a disseminated form of CMV disease including hepatitis and retinitis. Our results were greatly influenced by the impact of the highly active antiretroviral therapy that reduced incidence of CMV viremia and occurrence of CMV disease in the HIV infected patients. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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CMV Semiquantitative PCR HIV patients Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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CMV Semiquantitative PCR HIV patients Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Aldo de Albuquerque CUNHA Lauro Juliano MARIN Victor Hugo AQUINO Luiz Tadeu Moraes FIGUEIREDO Diagnosis of cytomegalovirus infections by qualitative and quantitative PCR in HIV infected patients |
topic_facet |
CMV Semiquantitative PCR HIV patients Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
A high incidence of cytomegalovirus (CMV) infections is observed in Brazil. These viruses are causatives of significant morbidity and mortality among patients with advanced human immunodeficiency virus (HIV) infection. This work, shows the application of a PCR on determination of CMV load in the buffy coat and plasma. We analyzed the samples of 247 HIV infected patients in order to diagnose CMV infection and disease. We developed a semi-quantitative PCR that amplifies part of the glycoprotein B (gB) gene of CMV. The semi-quantitative PCR was carried out only in positive clinical samples in a qualitative PCR confirmed by a nested-PCR. CD4 lymphocyte count, HIV viral load and CMV disease symptom were correlated with CMV load. CMV genome was detected in the buffy coat of 82 of 237 (34.6%) patients, in 10 of these the CMV load was determined varying between 928 and 332 880 viral copies/mug DNA. None of these 237 patients developed any suggestive manifestation of CMV disease. For the other 10 HIV infected patients selected based on the suspicion of CMV disease, CMV genome was detected in only one case. This patient presented a high CMV load, 8 000 000 copies/mug DNA, and developed a disseminated form of CMV disease including hepatitis and retinitis. Our results were greatly influenced by the impact of the highly active antiretroviral therapy that reduced incidence of CMV viremia and occurrence of CMV disease in the HIV infected patients. |
format |
Article in Journal/Newspaper |
author |
Aldo de Albuquerque CUNHA Lauro Juliano MARIN Victor Hugo AQUINO Luiz Tadeu Moraes FIGUEIREDO |
author_facet |
Aldo de Albuquerque CUNHA Lauro Juliano MARIN Victor Hugo AQUINO Luiz Tadeu Moraes FIGUEIREDO |
author_sort |
Aldo de Albuquerque CUNHA |
title |
Diagnosis of cytomegalovirus infections by qualitative and quantitative PCR in HIV infected patients |
title_short |
Diagnosis of cytomegalovirus infections by qualitative and quantitative PCR in HIV infected patients |
title_full |
Diagnosis of cytomegalovirus infections by qualitative and quantitative PCR in HIV infected patients |
title_fullStr |
Diagnosis of cytomegalovirus infections by qualitative and quantitative PCR in HIV infected patients |
title_full_unstemmed |
Diagnosis of cytomegalovirus infections by qualitative and quantitative PCR in HIV infected patients |
title_sort |
diagnosis of cytomegalovirus infections by qualitative and quantitative pcr in hiv infected patients |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2002 |
url |
https://doaj.org/article/0f63fc4e89364277b2b4380dfe39f7f6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 44, Iss 3, Pp 127-132 (2002) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652002000300003&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/0f63fc4e89364277b2b4380dfe39f7f6 |
_version_ |
1809896467318439936 |