Investigation of the Initial Host Response of Naïve Atlantic Salmon ( Salmo salar ) Inoculated with Paramoeba perurans
Amoebic Gill Disease (AGD), caused by the ectoparasite Paramoeba perurans is characterised by hyperplasia of the gill epithelium and lamellar fusion. In this study, the initial host response of naïve Atlantic salmon ( Salmo salar ) inoculated with P. perurans was investigated. Using gel-free proteom...
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2021
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ftdoajarticles:oai:doaj.org/article:0f6188812d2446eba9af34f11e8e1792 2023-05-15T15:30:47+02:00 Investigation of the Initial Host Response of Naïve Atlantic Salmon ( Salmo salar ) Inoculated with Paramoeba perurans Michelle McCormack Eugene Dillon Ian O’Connor Eugene MacCarthy 2021-04-01T00:00:00Z https://doi.org/10.3390/microorganisms9040746 https://doaj.org/article/0f6188812d2446eba9af34f11e8e1792 EN eng MDPI AG https://www.mdpi.com/2076-2607/9/4/746 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms9040746 2076-2607 https://doaj.org/article/0f6188812d2446eba9af34f11e8e1792 Microorganisms, Vol 9, Iss 746, p 746 (2021) amoebic gill disease Atlantic salmon gel-free proteomics gill proteome serum proteome Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/microorganisms9040746 2022-12-31T12:23:08Z Amoebic Gill Disease (AGD), caused by the ectoparasite Paramoeba perurans is characterised by hyperplasia of the gill epithelium and lamellar fusion. In this study, the initial host response of naïve Atlantic salmon ( Salmo salar ) inoculated with P. perurans was investigated. Using gel-free proteomic techniques and mass spectrometry gill and serum samples were analysed at 7 timepoints (2, 3, 4, 7, 9, 11 and 14 days) post-inoculation with P. perurans . Differential expression of immune related proteins was assessed by comparison of protein expression from each time point against naïve controls. Few host immune molecules associated with innate immunity showed increased expression in response to gill colonisation by amoebae. Furthermore, many proteins with roles in immune signalling, phagocytosis and T-cell proliferation were found to be inhibited upon disease progression. Initially, various immune factors demonstrated the anticipated increase in expression in response to infection in the serum while some immune inhibition became apparent at the later stages of disease progression. Taken together, the pro-immune trend observed in serum, the lack of a robust early immune response in the gill and the diversity of those proteins in the gill whose altered expression negatively impact the immune response, support the concept of a pathogen-derived suppression of the host response. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Microorganisms 9 4 746 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
amoebic gill disease Atlantic salmon gel-free proteomics gill proteome serum proteome Biology (General) QH301-705.5 |
spellingShingle |
amoebic gill disease Atlantic salmon gel-free proteomics gill proteome serum proteome Biology (General) QH301-705.5 Michelle McCormack Eugene Dillon Ian O’Connor Eugene MacCarthy Investigation of the Initial Host Response of Naïve Atlantic Salmon ( Salmo salar ) Inoculated with Paramoeba perurans |
topic_facet |
amoebic gill disease Atlantic salmon gel-free proteomics gill proteome serum proteome Biology (General) QH301-705.5 |
description |
Amoebic Gill Disease (AGD), caused by the ectoparasite Paramoeba perurans is characterised by hyperplasia of the gill epithelium and lamellar fusion. In this study, the initial host response of naïve Atlantic salmon ( Salmo salar ) inoculated with P. perurans was investigated. Using gel-free proteomic techniques and mass spectrometry gill and serum samples were analysed at 7 timepoints (2, 3, 4, 7, 9, 11 and 14 days) post-inoculation with P. perurans . Differential expression of immune related proteins was assessed by comparison of protein expression from each time point against naïve controls. Few host immune molecules associated with innate immunity showed increased expression in response to gill colonisation by amoebae. Furthermore, many proteins with roles in immune signalling, phagocytosis and T-cell proliferation were found to be inhibited upon disease progression. Initially, various immune factors demonstrated the anticipated increase in expression in response to infection in the serum while some immune inhibition became apparent at the later stages of disease progression. Taken together, the pro-immune trend observed in serum, the lack of a robust early immune response in the gill and the diversity of those proteins in the gill whose altered expression negatively impact the immune response, support the concept of a pathogen-derived suppression of the host response. |
format |
Article in Journal/Newspaper |
author |
Michelle McCormack Eugene Dillon Ian O’Connor Eugene MacCarthy |
author_facet |
Michelle McCormack Eugene Dillon Ian O’Connor Eugene MacCarthy |
author_sort |
Michelle McCormack |
title |
Investigation of the Initial Host Response of Naïve Atlantic Salmon ( Salmo salar ) Inoculated with Paramoeba perurans |
title_short |
Investigation of the Initial Host Response of Naïve Atlantic Salmon ( Salmo salar ) Inoculated with Paramoeba perurans |
title_full |
Investigation of the Initial Host Response of Naïve Atlantic Salmon ( Salmo salar ) Inoculated with Paramoeba perurans |
title_fullStr |
Investigation of the Initial Host Response of Naïve Atlantic Salmon ( Salmo salar ) Inoculated with Paramoeba perurans |
title_full_unstemmed |
Investigation of the Initial Host Response of Naïve Atlantic Salmon ( Salmo salar ) Inoculated with Paramoeba perurans |
title_sort |
investigation of the initial host response of naïve atlantic salmon ( salmo salar ) inoculated with paramoeba perurans |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/microorganisms9040746 https://doaj.org/article/0f6188812d2446eba9af34f11e8e1792 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Microorganisms, Vol 9, Iss 746, p 746 (2021) |
op_relation |
https://www.mdpi.com/2076-2607/9/4/746 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms9040746 2076-2607 https://doaj.org/article/0f6188812d2446eba9af34f11e8e1792 |
op_doi |
https://doi.org/10.3390/microorganisms9040746 |
container_title |
Microorganisms |
container_volume |
9 |
container_issue |
4 |
container_start_page |
746 |
_version_ |
1766361234594070528 |