Nested Spatial and Temporal Modeling of Environmental Conditions Associated With Genetic Markers of Vibrio parahaemolyticus in Washington State Pacific Oysters

The Pacific Northwest (PNW) is one of the largest commercial harvesting areas for Pacific oysters (Crassostrea gigas) in the United States. Vibrio parahaemolyticus, a bacterium naturally present in estuarine waters accumulates in shellfish and is a major cause of seafood-borne illness. Growers, cons...

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Published in:Frontiers in Microbiology
Main Authors: Brendan Fries, Benjamin J. K. Davis, Anne E. Corrigan, Angelo DePaola, Frank C. Curriero
Format: Article in Journal/Newspaper
Language:English
Published: Frontiers Media S.A. 2022
Subjects:
Online Access:https://doi.org/10.3389/fmicb.2022.849336
https://doaj.org/article/0f584bd7229b4f74a00f7ebf60199438
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spelling ftdoajarticles:oai:doaj.org/article:0f584bd7229b4f74a00f7ebf60199438 2023-05-15T15:58:27+02:00 Nested Spatial and Temporal Modeling of Environmental Conditions Associated With Genetic Markers of Vibrio parahaemolyticus in Washington State Pacific Oysters Brendan Fries Benjamin J. K. Davis Anne E. Corrigan Angelo DePaola Frank C. Curriero 2022-03-01T00:00:00Z https://doi.org/10.3389/fmicb.2022.849336 https://doaj.org/article/0f584bd7229b4f74a00f7ebf60199438 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmicb.2022.849336/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2022.849336 https://doaj.org/article/0f584bd7229b4f74a00f7ebf60199438 Frontiers in Microbiology, Vol 13 (2022) Vibrio parahaemolyticus Pacific oysters (Crassostrea gigas) spatial modeling temporal modeling mixed-effects model Washington (state) Microbiology QR1-502 article 2022 ftdoajarticles https://doi.org/10.3389/fmicb.2022.849336 2022-12-31T08:22:36Z The Pacific Northwest (PNW) is one of the largest commercial harvesting areas for Pacific oysters (Crassostrea gigas) in the United States. Vibrio parahaemolyticus, a bacterium naturally present in estuarine waters accumulates in shellfish and is a major cause of seafood-borne illness. Growers, consumers, and public-health officials have raised concerns about rising vibriosis cases in the region. Vibrio parahaemolyticus genetic markers (tlh, tdh, and trh) were estimated using an most-probable-number (MPN)-PCR technique in Washington State Pacific oysters regularly sampled between May and October from 2005 to 2019 (N = 2,836); environmental conditions were also measured at each sampling event. Multilevel mixed-effects regression models were used to assess relationships between environmental measures and genetic markers as well as genetic marker ratios (trh:tlh, tdh:tlh, and tdh:trh), accounting for variation across space and time. Spatial and temporal dependence were also accounted for in the model structure. Model fit improved when including environmental measures from previous weeks (1-week lag for air temperature, 3-week lag for salinity). Positive associations were found between tlh and surface water temp, specifically between 15 and 26°C, and between trh and surface water temperature up to 26°C. tlh and trh were negatively associated with 3-week lagged salinity in the most saline waters (> 27 ppt). There was also a positive relationship between tissue temperature and tdh, but only above 20°C. The tdh:tlh ratio displayed analogous inverted non-linear relationships as tlh. The non-linear associations found between the genetic targets and environmental measures demonstrate the complex habitat suitability of V. parahaemolyticus. Additional associations with both spatial and temporal variables also suggest there are influential unmeasured environmental conditions that could further explain bacterium variability. Overall, these findings confirm previous ecological risk factors for vibriosis in Washington State, ... Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Pacific Frontiers in Microbiology 13
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Vibrio parahaemolyticus
Pacific oysters (Crassostrea gigas)
spatial modeling
temporal modeling
mixed-effects model
Washington (state)
Microbiology
QR1-502
spellingShingle Vibrio parahaemolyticus
Pacific oysters (Crassostrea gigas)
spatial modeling
temporal modeling
mixed-effects model
Washington (state)
Microbiology
QR1-502
Brendan Fries
Benjamin J. K. Davis
Anne E. Corrigan
Angelo DePaola
Frank C. Curriero
Nested Spatial and Temporal Modeling of Environmental Conditions Associated With Genetic Markers of Vibrio parahaemolyticus in Washington State Pacific Oysters
topic_facet Vibrio parahaemolyticus
Pacific oysters (Crassostrea gigas)
spatial modeling
temporal modeling
mixed-effects model
Washington (state)
Microbiology
QR1-502
description The Pacific Northwest (PNW) is one of the largest commercial harvesting areas for Pacific oysters (Crassostrea gigas) in the United States. Vibrio parahaemolyticus, a bacterium naturally present in estuarine waters accumulates in shellfish and is a major cause of seafood-borne illness. Growers, consumers, and public-health officials have raised concerns about rising vibriosis cases in the region. Vibrio parahaemolyticus genetic markers (tlh, tdh, and trh) were estimated using an most-probable-number (MPN)-PCR technique in Washington State Pacific oysters regularly sampled between May and October from 2005 to 2019 (N = 2,836); environmental conditions were also measured at each sampling event. Multilevel mixed-effects regression models were used to assess relationships between environmental measures and genetic markers as well as genetic marker ratios (trh:tlh, tdh:tlh, and tdh:trh), accounting for variation across space and time. Spatial and temporal dependence were also accounted for in the model structure. Model fit improved when including environmental measures from previous weeks (1-week lag for air temperature, 3-week lag for salinity). Positive associations were found between tlh and surface water temp, specifically between 15 and 26°C, and between trh and surface water temperature up to 26°C. tlh and trh were negatively associated with 3-week lagged salinity in the most saline waters (> 27 ppt). There was also a positive relationship between tissue temperature and tdh, but only above 20°C. The tdh:tlh ratio displayed analogous inverted non-linear relationships as tlh. The non-linear associations found between the genetic targets and environmental measures demonstrate the complex habitat suitability of V. parahaemolyticus. Additional associations with both spatial and temporal variables also suggest there are influential unmeasured environmental conditions that could further explain bacterium variability. Overall, these findings confirm previous ecological risk factors for vibriosis in Washington State, ...
format Article in Journal/Newspaper
author Brendan Fries
Benjamin J. K. Davis
Anne E. Corrigan
Angelo DePaola
Frank C. Curriero
author_facet Brendan Fries
Benjamin J. K. Davis
Anne E. Corrigan
Angelo DePaola
Frank C. Curriero
author_sort Brendan Fries
title Nested Spatial and Temporal Modeling of Environmental Conditions Associated With Genetic Markers of Vibrio parahaemolyticus in Washington State Pacific Oysters
title_short Nested Spatial and Temporal Modeling of Environmental Conditions Associated With Genetic Markers of Vibrio parahaemolyticus in Washington State Pacific Oysters
title_full Nested Spatial and Temporal Modeling of Environmental Conditions Associated With Genetic Markers of Vibrio parahaemolyticus in Washington State Pacific Oysters
title_fullStr Nested Spatial and Temporal Modeling of Environmental Conditions Associated With Genetic Markers of Vibrio parahaemolyticus in Washington State Pacific Oysters
title_full_unstemmed Nested Spatial and Temporal Modeling of Environmental Conditions Associated With Genetic Markers of Vibrio parahaemolyticus in Washington State Pacific Oysters
title_sort nested spatial and temporal modeling of environmental conditions associated with genetic markers of vibrio parahaemolyticus in washington state pacific oysters
publisher Frontiers Media S.A.
publishDate 2022
url https://doi.org/10.3389/fmicb.2022.849336
https://doaj.org/article/0f584bd7229b4f74a00f7ebf60199438
geographic Pacific
geographic_facet Pacific
genre Crassostrea gigas
genre_facet Crassostrea gigas
op_source Frontiers in Microbiology, Vol 13 (2022)
op_relation https://www.frontiersin.org/articles/10.3389/fmicb.2022.849336/full
https://doaj.org/toc/1664-302X
1664-302X
doi:10.3389/fmicb.2022.849336
https://doaj.org/article/0f584bd7229b4f74a00f7ebf60199438
op_doi https://doi.org/10.3389/fmicb.2022.849336
container_title Frontiers in Microbiology
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