Lack of significant recovery of chloroquine sensitivity in Plasmodium falciparum parasites following discontinuance of chloroquine use in Papua New Guinea
Abstract Background Chloroquine treatment for Plasmodium falciparum has been discontinued in almost all endemic regions due to the spread of resistant isolates. Reversal of chloroquine susceptibility after chloroquine discontinuation has been reported in dozens of endemic regions. However, this phen...
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ftdoajarticles:oai:doaj.org/article:0f22cbd8a86445a68061ed4aa03a3ed4 2023-05-15T15:09:20+02:00 Lack of significant recovery of chloroquine sensitivity in Plasmodium falciparum parasites following discontinuance of chloroquine use in Papua New Guinea Makoto Sekihara Shin-Ichiro Tachibana Masato Yamauchi Shoki Yatsushiro Steven Tiwara Naoyuki Fukuda Mie Ikeda Toshiyuki Mori Makoto Hirai Francis Hombhanje Toshihiro Mita 2018-11-01T00:00:00Z https://doi.org/10.1186/s12936-018-2585-x https://doaj.org/article/0f22cbd8a86445a68061ed4aa03a3ed4 EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2585-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2585-x 1475-2875 https://doaj.org/article/0f22cbd8a86445a68061ed4aa03a3ed4 Malaria Journal, Vol 17, Iss 1, Pp 1-10 (2018) Plasmodium falciparum Chloroquine Resistance Recovery pfcrt Fitness Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2585-x 2022-12-31T12:02:16Z Abstract Background Chloroquine treatment for Plasmodium falciparum has been discontinued in almost all endemic regions due to the spread of resistant isolates. Reversal of chloroquine susceptibility after chloroquine discontinuation has been reported in dozens of endemic regions. However, this phenomenon has been mostly observed in Africa and is not well documented in other malaria endemic regions. To investigate this, an ex vivo study on susceptibility to chloroquine and lumefantrine was conducted during 2016–2018 in Wewak, Papua New Guinea where chloroquine had been removed from the official malaria treatment regimen in 2010. Genotyping of pfcrt and pfmdr1 was also performed. Results In total, 368 patients were enrolled in this study. Average IC50 values for chloroquine were 106.6, 80.5, and 87.6 nM in 2016, 2017, and 2018, respectively. These values were not significantly changed from those obtained in 2002/2003 (108 nM). The majority of parasites harboured a pfcrt K76T the mutation responsible for chloroquine resistance. However, a significant upward trend was observed in the frequency of the K76 (wild) allele from 2.3% in 2016 to 11.7% in 2018 (P = 0.008; Cochran–Armitage trend test). Conclusions Eight years of chloroquine withdrawal has not induced a significant recovery of susceptibility in Papua New Guinea. However, an increasing tendency of parasites harbouring chloroquine-susceptible K76 suggests a possibility of resurgence of chloroquine susceptibility in the future. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Armitage ENVELOPE(166.667,166.667,-77.850,-77.850) Malaria Journal 17 1 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Plasmodium falciparum Chloroquine Resistance Recovery pfcrt Fitness Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Plasmodium falciparum Chloroquine Resistance Recovery pfcrt Fitness Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Makoto Sekihara Shin-Ichiro Tachibana Masato Yamauchi Shoki Yatsushiro Steven Tiwara Naoyuki Fukuda Mie Ikeda Toshiyuki Mori Makoto Hirai Francis Hombhanje Toshihiro Mita Lack of significant recovery of chloroquine sensitivity in Plasmodium falciparum parasites following discontinuance of chloroquine use in Papua New Guinea |
topic_facet |
Plasmodium falciparum Chloroquine Resistance Recovery pfcrt Fitness Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Chloroquine treatment for Plasmodium falciparum has been discontinued in almost all endemic regions due to the spread of resistant isolates. Reversal of chloroquine susceptibility after chloroquine discontinuation has been reported in dozens of endemic regions. However, this phenomenon has been mostly observed in Africa and is not well documented in other malaria endemic regions. To investigate this, an ex vivo study on susceptibility to chloroquine and lumefantrine was conducted during 2016–2018 in Wewak, Papua New Guinea where chloroquine had been removed from the official malaria treatment regimen in 2010. Genotyping of pfcrt and pfmdr1 was also performed. Results In total, 368 patients were enrolled in this study. Average IC50 values for chloroquine were 106.6, 80.5, and 87.6 nM in 2016, 2017, and 2018, respectively. These values were not significantly changed from those obtained in 2002/2003 (108 nM). The majority of parasites harboured a pfcrt K76T the mutation responsible for chloroquine resistance. However, a significant upward trend was observed in the frequency of the K76 (wild) allele from 2.3% in 2016 to 11.7% in 2018 (P = 0.008; Cochran–Armitage trend test). Conclusions Eight years of chloroquine withdrawal has not induced a significant recovery of susceptibility in Papua New Guinea. However, an increasing tendency of parasites harbouring chloroquine-susceptible K76 suggests a possibility of resurgence of chloroquine susceptibility in the future. |
format |
Article in Journal/Newspaper |
author |
Makoto Sekihara Shin-Ichiro Tachibana Masato Yamauchi Shoki Yatsushiro Steven Tiwara Naoyuki Fukuda Mie Ikeda Toshiyuki Mori Makoto Hirai Francis Hombhanje Toshihiro Mita |
author_facet |
Makoto Sekihara Shin-Ichiro Tachibana Masato Yamauchi Shoki Yatsushiro Steven Tiwara Naoyuki Fukuda Mie Ikeda Toshiyuki Mori Makoto Hirai Francis Hombhanje Toshihiro Mita |
author_sort |
Makoto Sekihara |
title |
Lack of significant recovery of chloroquine sensitivity in Plasmodium falciparum parasites following discontinuance of chloroquine use in Papua New Guinea |
title_short |
Lack of significant recovery of chloroquine sensitivity in Plasmodium falciparum parasites following discontinuance of chloroquine use in Papua New Guinea |
title_full |
Lack of significant recovery of chloroquine sensitivity in Plasmodium falciparum parasites following discontinuance of chloroquine use in Papua New Guinea |
title_fullStr |
Lack of significant recovery of chloroquine sensitivity in Plasmodium falciparum parasites following discontinuance of chloroquine use in Papua New Guinea |
title_full_unstemmed |
Lack of significant recovery of chloroquine sensitivity in Plasmodium falciparum parasites following discontinuance of chloroquine use in Papua New Guinea |
title_sort |
lack of significant recovery of chloroquine sensitivity in plasmodium falciparum parasites following discontinuance of chloroquine use in papua new guinea |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2585-x https://doaj.org/article/0f22cbd8a86445a68061ed4aa03a3ed4 |
long_lat |
ENVELOPE(166.667,166.667,-77.850,-77.850) |
geographic |
Arctic Armitage |
geographic_facet |
Arctic Armitage |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-10 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2585-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2585-x 1475-2875 https://doaj.org/article/0f22cbd8a86445a68061ed4aa03a3ed4 |
op_doi |
https://doi.org/10.1186/s12936-018-2585-x |
container_title |
Malaria Journal |
container_volume |
17 |
container_issue |
1 |
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1766340546550300672 |