Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus
Objective: To determine the inhibition mechanisms of secretome protein extracted from Paenibacillus polymyxa Kp10 (Kp10) and Lactococcus lactis Gh1 (Gh1) against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE). Methods: The sensitivity and viability of...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Language: | English |
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Wolters Kluwer Medknow Publications
2022
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Online Access: | https://doi.org/10.4103/2221-1691.360564 https://doaj.org/article/0f15a9db5fbc48eabe5bd117993c8836 |
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ftdoajarticles:oai:doaj.org/article:0f15a9db5fbc48eabe5bd117993c8836 2023-05-15T15:10:02+02:00 Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus Nurul Hana Zainal Baharin Nur Fadhilah Khairil Mokhtar Mohd Nasir Mohd Desa Nurul Diana Dzaraly Abdul Rahman Muthanna Mazen M. Jamil Al-Obaidi Mohd Hafis Yuswan Sahar Abbasiliasi Norasfaliza Rahmad Wan Ahmad Kamil Wan Nur Ismah Amalia Mohd Hashim Shuhaimi Mustafa 2022-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.360564 https://doaj.org/article/0f15a9db5fbc48eabe5bd117993c8836 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=11;spage=483;epage=494;aulast=Zainal https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.360564 https://doaj.org/article/0f15a9db5fbc48eabe5bd117993c8836 Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 11, Pp 483-494 (2022) antimicrobial proteins secretome proteins antibiotic-resistance paenibacillus polymyxa kp10 lactococcus lactis gh1 vancomycin-resistant enterococcus methicillin-resistant staphylococcus aureus mechanism Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.4103/2221-1691.360564 2022-12-30T20:04:21Z Objective: To determine the inhibition mechanisms of secretome protein extracted from Paenibacillus polymyxa Kp10 (Kp10) and Lactococcus lactis Gh1 (Gh1) against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE). Methods: The sensitivity and viability of MRSA and VRE treated with secretome proteins of Kp10 and Gh1 were determined using minimal inhibitory concentration, minimum bactericidal concentration, and time-to-kill assays. The morphological changes were observed using scanning electron microscopy and transmission electron microscopy. To elucidate the antimicrobial mechanism of secretome protein of Kp10 and Gh1 against MRSA and VRE, 2D gel proteomic analysis using liquid chromatography-mass spectrometry was run by comparing upregulated and downregulated proteins, and the proton motive force study including the efflux of ATP, pH gradient, and the membrane potential study were conducted. Results: MRSA and VRE were sensitive to Kp10 and Gh1 secretome protein extracts and displayed apparent morphological and internal composition changes. Several proteins associated with cellular component functions were either downregulated or upregulated in treated MRSA and VRE by changing the membrane potential gradient. Conclusions: Kp10 and Gh1 secretome proteins reduce the growth of VRE and MRSA by damaging the cell membrane. Cell division, cell wall biosynthesis, and protein synthesis are involved in the inhibition mechanism. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 12 11 483 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
antimicrobial proteins secretome proteins antibiotic-resistance paenibacillus polymyxa kp10 lactococcus lactis gh1 vancomycin-resistant enterococcus methicillin-resistant staphylococcus aureus mechanism Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
antimicrobial proteins secretome proteins antibiotic-resistance paenibacillus polymyxa kp10 lactococcus lactis gh1 vancomycin-resistant enterococcus methicillin-resistant staphylococcus aureus mechanism Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Nurul Hana Zainal Baharin Nur Fadhilah Khairil Mokhtar Mohd Nasir Mohd Desa Nurul Diana Dzaraly Abdul Rahman Muthanna Mazen M. Jamil Al-Obaidi Mohd Hafis Yuswan Sahar Abbasiliasi Norasfaliza Rahmad Wan Ahmad Kamil Wan Nur Ismah Amalia Mohd Hashim Shuhaimi Mustafa Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus |
topic_facet |
antimicrobial proteins secretome proteins antibiotic-resistance paenibacillus polymyxa kp10 lactococcus lactis gh1 vancomycin-resistant enterococcus methicillin-resistant staphylococcus aureus mechanism Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To determine the inhibition mechanisms of secretome protein extracted from Paenibacillus polymyxa Kp10 (Kp10) and Lactococcus lactis Gh1 (Gh1) against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE). Methods: The sensitivity and viability of MRSA and VRE treated with secretome proteins of Kp10 and Gh1 were determined using minimal inhibitory concentration, minimum bactericidal concentration, and time-to-kill assays. The morphological changes were observed using scanning electron microscopy and transmission electron microscopy. To elucidate the antimicrobial mechanism of secretome protein of Kp10 and Gh1 against MRSA and VRE, 2D gel proteomic analysis using liquid chromatography-mass spectrometry was run by comparing upregulated and downregulated proteins, and the proton motive force study including the efflux of ATP, pH gradient, and the membrane potential study were conducted. Results: MRSA and VRE were sensitive to Kp10 and Gh1 secretome protein extracts and displayed apparent morphological and internal composition changes. Several proteins associated with cellular component functions were either downregulated or upregulated in treated MRSA and VRE by changing the membrane potential gradient. Conclusions: Kp10 and Gh1 secretome proteins reduce the growth of VRE and MRSA by damaging the cell membrane. Cell division, cell wall biosynthesis, and protein synthesis are involved in the inhibition mechanism. |
format |
Article in Journal/Newspaper |
author |
Nurul Hana Zainal Baharin Nur Fadhilah Khairil Mokhtar Mohd Nasir Mohd Desa Nurul Diana Dzaraly Abdul Rahman Muthanna Mazen M. Jamil Al-Obaidi Mohd Hafis Yuswan Sahar Abbasiliasi Norasfaliza Rahmad Wan Ahmad Kamil Wan Nur Ismah Amalia Mohd Hashim Shuhaimi Mustafa |
author_facet |
Nurul Hana Zainal Baharin Nur Fadhilah Khairil Mokhtar Mohd Nasir Mohd Desa Nurul Diana Dzaraly Abdul Rahman Muthanna Mazen M. Jamil Al-Obaidi Mohd Hafis Yuswan Sahar Abbasiliasi Norasfaliza Rahmad Wan Ahmad Kamil Wan Nur Ismah Amalia Mohd Hashim Shuhaimi Mustafa |
author_sort |
Nurul Hana Zainal Baharin |
title |
Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus |
title_short |
Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus |
title_full |
Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus |
title_fullStr |
Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus |
title_full_unstemmed |
Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus |
title_sort |
inhibition mechanisms of secretome proteins from paenibacillus polymyxa kp10 and lactococcus lactis gh1 against methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococcus |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2022 |
url |
https://doi.org/10.4103/2221-1691.360564 https://doaj.org/article/0f15a9db5fbc48eabe5bd117993c8836 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 11, Pp 483-494 (2022) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=11;spage=483;epage=494;aulast=Zainal https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.360564 https://doaj.org/article/0f15a9db5fbc48eabe5bd117993c8836 |
op_doi |
https://doi.org/10.4103/2221-1691.360564 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
12 |
container_issue |
11 |
container_start_page |
483 |
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1766341101286850560 |