Molecular Biomarkers of the Mitochondrial Quality Control Are Differently Affected by Hypoxia-Reoxygenation Stress in Marine Bivalves Crassostrea gigas and Mytilus edulis
Coastal environments commonly experience strong oxygen fluctuations. Resulting hypoxia/reoxygenation stress can negatively affect mitochondrial functions, since oxygen deficiency impairs ATP generation, whereas a surge of oxygen causes mitochondrial damage by oxidative stress. Marine intertidal biva...
Published in: | Frontiers in Marine Science |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2020
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fmars.2020.604411 https://doaj.org/article/0ee5d0a25d9346ce8fc646cd02045665 |
id |
ftdoajarticles:oai:doaj.org/article:0ee5d0a25d9346ce8fc646cd02045665 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:0ee5d0a25d9346ce8fc646cd02045665 2023-05-15T15:58:25+02:00 Molecular Biomarkers of the Mitochondrial Quality Control Are Differently Affected by Hypoxia-Reoxygenation Stress in Marine Bivalves Crassostrea gigas and Mytilus edulis Jennifer B. M. Steffen Halina I. Falfushynska Helen Piontkivska Inna M. Sokolova 2020-12-01T00:00:00Z https://doi.org/10.3389/fmars.2020.604411 https://doaj.org/article/0ee5d0a25d9346ce8fc646cd02045665 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmars.2020.604411/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2020.604411 https://doaj.org/article/0ee5d0a25d9346ce8fc646cd02045665 Frontiers in Marine Science, Vol 7 (2020) mitochondrial fusion and fission quantitative real time PCR (qPCR) mRNA expression mollusca proteolysis mitophagy Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2020 ftdoajarticles https://doi.org/10.3389/fmars.2020.604411 2022-12-31T04:25:25Z Coastal environments commonly experience strong oxygen fluctuations. Resulting hypoxia/reoxygenation stress can negatively affect mitochondrial functions, since oxygen deficiency impairs ATP generation, whereas a surge of oxygen causes mitochondrial damage by oxidative stress. Marine intertidal bivalves are adapted to fluctuating oxygen conditions, yet the underlying molecular mechanisms that sustain mitochondrial integrity and function during oxygen fluctuations are not yet well understood. We used targeted mRNA expression analysis to determine the potential involvement of the mitochondrial quality control mechanisms in responses to short-term hypoxia (24 h at <0.01% O2) and subsequent reoxygenation (1.5 h at 21% O2) in two hypoxia-tolerant marine bivalves, the Pacific oysters Crassostrea gigas and the blue mussels Mytilus edulis. We hypothesized that the genes involved in the mitochondrial quality control will be upregulated during hypoxia, and the less hypoxia-tolerant of the two studied species (M. edulis) will show a stronger dependence on transcriptional upregulation of these pathways than C. gigas. To test these hypotheses, mRNA expression of 17 (C. gigas) and 11 (M. edulis) marker genes involved in mitochondrial fusion, fission, proteolysis and mitophagy was analyzed in the digestive gland of M. edulis and C. gigas in normoxia and during hypoxia-reoxygenation (H/R) stress. In the mussels, the mRNA expression of the transcripts related to mitochondrial dynamics and quality control was strongly altered during H/R stress showing a shift toward fission, suppression of fusion, an increase in mitochondrial proteolysis and onset of mitophagy. These changes indicate that H/R stress induces mitochondrial injury in M. edulis requiring upregulation of the protective mechanisms to segregate the dysfunctional mitochondria by fission and degrade the oxidative damaged proteins and/or organelles. Unlike mussels, the transcript levels of all studied genes in the oysters remained at the baseline (normoxic) levels ... Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Pacific Frontiers in Marine Science 7 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
mitochondrial fusion and fission quantitative real time PCR (qPCR) mRNA expression mollusca proteolysis mitophagy Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
mitochondrial fusion and fission quantitative real time PCR (qPCR) mRNA expression mollusca proteolysis mitophagy Science Q General. Including nature conservation geographical distribution QH1-199.5 Jennifer B. M. Steffen Halina I. Falfushynska Helen Piontkivska Inna M. Sokolova Molecular Biomarkers of the Mitochondrial Quality Control Are Differently Affected by Hypoxia-Reoxygenation Stress in Marine Bivalves Crassostrea gigas and Mytilus edulis |
topic_facet |
mitochondrial fusion and fission quantitative real time PCR (qPCR) mRNA expression mollusca proteolysis mitophagy Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
Coastal environments commonly experience strong oxygen fluctuations. Resulting hypoxia/reoxygenation stress can negatively affect mitochondrial functions, since oxygen deficiency impairs ATP generation, whereas a surge of oxygen causes mitochondrial damage by oxidative stress. Marine intertidal bivalves are adapted to fluctuating oxygen conditions, yet the underlying molecular mechanisms that sustain mitochondrial integrity and function during oxygen fluctuations are not yet well understood. We used targeted mRNA expression analysis to determine the potential involvement of the mitochondrial quality control mechanisms in responses to short-term hypoxia (24 h at <0.01% O2) and subsequent reoxygenation (1.5 h at 21% O2) in two hypoxia-tolerant marine bivalves, the Pacific oysters Crassostrea gigas and the blue mussels Mytilus edulis. We hypothesized that the genes involved in the mitochondrial quality control will be upregulated during hypoxia, and the less hypoxia-tolerant of the two studied species (M. edulis) will show a stronger dependence on transcriptional upregulation of these pathways than C. gigas. To test these hypotheses, mRNA expression of 17 (C. gigas) and 11 (M. edulis) marker genes involved in mitochondrial fusion, fission, proteolysis and mitophagy was analyzed in the digestive gland of M. edulis and C. gigas in normoxia and during hypoxia-reoxygenation (H/R) stress. In the mussels, the mRNA expression of the transcripts related to mitochondrial dynamics and quality control was strongly altered during H/R stress showing a shift toward fission, suppression of fusion, an increase in mitochondrial proteolysis and onset of mitophagy. These changes indicate that H/R stress induces mitochondrial injury in M. edulis requiring upregulation of the protective mechanisms to segregate the dysfunctional mitochondria by fission and degrade the oxidative damaged proteins and/or organelles. Unlike mussels, the transcript levels of all studied genes in the oysters remained at the baseline (normoxic) levels ... |
format |
Article in Journal/Newspaper |
author |
Jennifer B. M. Steffen Halina I. Falfushynska Helen Piontkivska Inna M. Sokolova |
author_facet |
Jennifer B. M. Steffen Halina I. Falfushynska Helen Piontkivska Inna M. Sokolova |
author_sort |
Jennifer B. M. Steffen |
title |
Molecular Biomarkers of the Mitochondrial Quality Control Are Differently Affected by Hypoxia-Reoxygenation Stress in Marine Bivalves Crassostrea gigas and Mytilus edulis |
title_short |
Molecular Biomarkers of the Mitochondrial Quality Control Are Differently Affected by Hypoxia-Reoxygenation Stress in Marine Bivalves Crassostrea gigas and Mytilus edulis |
title_full |
Molecular Biomarkers of the Mitochondrial Quality Control Are Differently Affected by Hypoxia-Reoxygenation Stress in Marine Bivalves Crassostrea gigas and Mytilus edulis |
title_fullStr |
Molecular Biomarkers of the Mitochondrial Quality Control Are Differently Affected by Hypoxia-Reoxygenation Stress in Marine Bivalves Crassostrea gigas and Mytilus edulis |
title_full_unstemmed |
Molecular Biomarkers of the Mitochondrial Quality Control Are Differently Affected by Hypoxia-Reoxygenation Stress in Marine Bivalves Crassostrea gigas and Mytilus edulis |
title_sort |
molecular biomarkers of the mitochondrial quality control are differently affected by hypoxia-reoxygenation stress in marine bivalves crassostrea gigas and mytilus edulis |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmars.2020.604411 https://doaj.org/article/0ee5d0a25d9346ce8fc646cd02045665 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Frontiers in Marine Science, Vol 7 (2020) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmars.2020.604411/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2020.604411 https://doaj.org/article/0ee5d0a25d9346ce8fc646cd02045665 |
op_doi |
https://doi.org/10.3389/fmars.2020.604411 |
container_title |
Frontiers in Marine Science |
container_volume |
7 |
_version_ |
1766394169102696448 |