Whole genome sequence of Vibrio cholerae directly from dried spotted filter paper.
Background Global estimates for cholera annually approximate 4 million cases worldwide with 95,000 deaths. Recent outbreaks, including Haiti and Yemen, are reminders that cholera is still a global health concern. Cholera outbreaks can rapidly induce high death tolls by overwhelming the capacity of h...
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ftdoajarticles:oai:doaj.org/article:0edefdd3d4a04231b77f74a8b89429ea 2023-05-15T15:15:13+02:00 Whole genome sequence of Vibrio cholerae directly from dried spotted filter paper. Angèle H M Bénard Etienne Guenou Maria Fookes Jerome Ateudjieu Watipaso Kasambara Matthew Siever Stanislas Rebaudet Jacques Boncy Paul Adrien Renaud Piarroux David A Sack Nicholas Thomson Amanda K Debes 2019-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007330 https://doaj.org/article/0edefdd3d4a04231b77f74a8b89429ea EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007330 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007330 https://doaj.org/article/0edefdd3d4a04231b77f74a8b89429ea PLoS Neglected Tropical Diseases, Vol 13, Iss 5, p e0007330 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007330 2022-12-31T08:00:29Z Background Global estimates for cholera annually approximate 4 million cases worldwide with 95,000 deaths. Recent outbreaks, including Haiti and Yemen, are reminders that cholera is still a global health concern. Cholera outbreaks can rapidly induce high death tolls by overwhelming the capacity of health facilities, especially in remote areas or areas of civil unrest. Recent studies demonstrated that stool specimens preserved on filter paper facilitate molecular analysis of Vibrio cholerae in resource limited settings. Specimens preserved in a rapid, low-cost, safe and sustainable manner for sequencing provides previously unavailable data about circulating cholera strains. This may ultimately contribute new information to shape public policy response on cholera control and elimination. Methodology/principal findings Whole genome sequencing (WGS) recovered close to a complete sequence of the V. cholerae O1 genome with satisfactory genome coverage from stool specimens enriched in alkaline peptone water (APW) and V. cholerae culture isolates, both spotted on filter paper. The minimum concentration of V. cholerae DNA sufficient to produce quality genomic information was 0.02 ng/μL. The genomic data confirmed the presence or absence of genes of epidemiological interest, including cholera toxin and pilus loci. WGS identified a variety of diarrheal pathogens from APW-enriched specimen spotted filter paper, highlighting the potential for this technique to explore the gut microbiome, potentially identifying co-infections, which may impact the severity of disease. WGS demonstrated that these specimens fit within the current global cholera phylogenetic tree, identifying the strains as the 7th pandemic El Tor. Conclusions WGS results allowed for mapping of short reads from APW-enriched specimen and culture isolate spotted filter papers. This provided valuable molecular epidemiological sequence information on V. cholerae strains from remote, low-resource settings. These results identified the presence of co-infecting ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 5 e0007330 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Angèle H M Bénard Etienne Guenou Maria Fookes Jerome Ateudjieu Watipaso Kasambara Matthew Siever Stanislas Rebaudet Jacques Boncy Paul Adrien Renaud Piarroux David A Sack Nicholas Thomson Amanda K Debes Whole genome sequence of Vibrio cholerae directly from dried spotted filter paper. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Global estimates for cholera annually approximate 4 million cases worldwide with 95,000 deaths. Recent outbreaks, including Haiti and Yemen, are reminders that cholera is still a global health concern. Cholera outbreaks can rapidly induce high death tolls by overwhelming the capacity of health facilities, especially in remote areas or areas of civil unrest. Recent studies demonstrated that stool specimens preserved on filter paper facilitate molecular analysis of Vibrio cholerae in resource limited settings. Specimens preserved in a rapid, low-cost, safe and sustainable manner for sequencing provides previously unavailable data about circulating cholera strains. This may ultimately contribute new information to shape public policy response on cholera control and elimination. Methodology/principal findings Whole genome sequencing (WGS) recovered close to a complete sequence of the V. cholerae O1 genome with satisfactory genome coverage from stool specimens enriched in alkaline peptone water (APW) and V. cholerae culture isolates, both spotted on filter paper. The minimum concentration of V. cholerae DNA sufficient to produce quality genomic information was 0.02 ng/μL. The genomic data confirmed the presence or absence of genes of epidemiological interest, including cholera toxin and pilus loci. WGS identified a variety of diarrheal pathogens from APW-enriched specimen spotted filter paper, highlighting the potential for this technique to explore the gut microbiome, potentially identifying co-infections, which may impact the severity of disease. WGS demonstrated that these specimens fit within the current global cholera phylogenetic tree, identifying the strains as the 7th pandemic El Tor. Conclusions WGS results allowed for mapping of short reads from APW-enriched specimen and culture isolate spotted filter papers. This provided valuable molecular epidemiological sequence information on V. cholerae strains from remote, low-resource settings. These results identified the presence of co-infecting ... |
format |
Article in Journal/Newspaper |
author |
Angèle H M Bénard Etienne Guenou Maria Fookes Jerome Ateudjieu Watipaso Kasambara Matthew Siever Stanislas Rebaudet Jacques Boncy Paul Adrien Renaud Piarroux David A Sack Nicholas Thomson Amanda K Debes |
author_facet |
Angèle H M Bénard Etienne Guenou Maria Fookes Jerome Ateudjieu Watipaso Kasambara Matthew Siever Stanislas Rebaudet Jacques Boncy Paul Adrien Renaud Piarroux David A Sack Nicholas Thomson Amanda K Debes |
author_sort |
Angèle H M Bénard |
title |
Whole genome sequence of Vibrio cholerae directly from dried spotted filter paper. |
title_short |
Whole genome sequence of Vibrio cholerae directly from dried spotted filter paper. |
title_full |
Whole genome sequence of Vibrio cholerae directly from dried spotted filter paper. |
title_fullStr |
Whole genome sequence of Vibrio cholerae directly from dried spotted filter paper. |
title_full_unstemmed |
Whole genome sequence of Vibrio cholerae directly from dried spotted filter paper. |
title_sort |
whole genome sequence of vibrio cholerae directly from dried spotted filter paper. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007330 https://doaj.org/article/0edefdd3d4a04231b77f74a8b89429ea |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 5, p e0007330 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007330 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007330 https://doaj.org/article/0edefdd3d4a04231b77f74a8b89429ea |
op_doi |
https://doi.org/10.1371/journal.pntd.0007330 |
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PLOS Neglected Tropical Diseases |
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13 |
container_issue |
5 |
container_start_page |
e0007330 |
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