Green synthesis of isopropyl myristate in novel single phase medium Part I: Batch optimization studies
Isopropyl myristate finds many applications in food, cosmetic and pharmaceutical industries as an emollient, thickening agent, or lubricant. Using a homogeneous reaction phase, non-specific lipase derived from Candida antartica, marketed as Novozym 435, was determined to be most suitable for the enz...
Published in: | Biotechnology Reports |
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Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier
2015
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Online Access: | https://doi.org/10.1016/j.btre.2015.10.006 https://doaj.org/article/0eddb5e3e6bd41ca9705000349b96752 |
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author | Rajeshkumar N. Vadgama Annamma A. Odaneth Arvind M. Lali |
author_facet | Rajeshkumar N. Vadgama Annamma A. Odaneth Arvind M. Lali |
author_sort | Rajeshkumar N. Vadgama |
collection | Directory of Open Access Journals: DOAJ Articles |
container_start_page | 133 |
container_title | Biotechnology Reports |
container_volume | 8 |
description | Isopropyl myristate finds many applications in food, cosmetic and pharmaceutical industries as an emollient, thickening agent, or lubricant. Using a homogeneous reaction phase, non-specific lipase derived from Candida antartica, marketed as Novozym 435, was determined to be most suitable for the enzymatic synthesis of isopropyl myristate. The high molar ratio of alcohol to acid creates novel single phase medium which overcomes mass transfer effects and facilitates downstream processing. The effect of various reaction parameters was optimized to obtain a high yield of isopropyl myristate. Effect of temperature, agitation speed, organic solvent, biocatalyst loading and batch operational stability of the enzyme was systematically studied. The conversion of 87.65% was obtained when the molar ratio of isopropyl alcohol to myristic acid (15:1) was used with 4% (w/w) catalyst loading and agitation speed of 150 rpm at 60 °C. The enzyme has also shown good batch operational stability under optimized conditions. |
format | Article in Journal/Newspaper |
genre | antartic* |
genre_facet | antartic* |
id | ftdoajarticles:oai:doaj.org/article:0eddb5e3e6bd41ca9705000349b96752 |
institution | Open Polar |
language | English |
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op_container_end_page | 137 |
op_doi | https://doi.org/10.1016/j.btre.2015.10.006 |
op_relation | http://www.sciencedirect.com/science/article/pii/S2215017X15000600 https://doaj.org/toc/2215-017X 2215-017X doi:10.1016/j.btre.2015.10.006 https://doaj.org/article/0eddb5e3e6bd41ca9705000349b96752 |
op_source | Biotechnology Reports, Vol 8, Iss C, Pp 133-137 (2015) |
publishDate | 2015 |
publisher | Elsevier |
record_format | openpolar |
spelling | ftdoajarticles:oai:doaj.org/article:0eddb5e3e6bd41ca9705000349b96752 2025-01-16T19:45:25+00:00 Green synthesis of isopropyl myristate in novel single phase medium Part I: Batch optimization studies Rajeshkumar N. Vadgama Annamma A. Odaneth Arvind M. Lali 2015-12-01T00:00:00Z https://doi.org/10.1016/j.btre.2015.10.006 https://doaj.org/article/0eddb5e3e6bd41ca9705000349b96752 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2215017X15000600 https://doaj.org/toc/2215-017X 2215-017X doi:10.1016/j.btre.2015.10.006 https://doaj.org/article/0eddb5e3e6bd41ca9705000349b96752 Biotechnology Reports, Vol 8, Iss C, Pp 133-137 (2015) Isopropyl myristate Myristic acid Isopropyl alcohol Novozym 435 Esterification Biotechnology TP248.13-248.65 article 2015 ftdoajarticles https://doi.org/10.1016/j.btre.2015.10.006 2022-12-31T01:49:36Z Isopropyl myristate finds many applications in food, cosmetic and pharmaceutical industries as an emollient, thickening agent, or lubricant. Using a homogeneous reaction phase, non-specific lipase derived from Candida antartica, marketed as Novozym 435, was determined to be most suitable for the enzymatic synthesis of isopropyl myristate. The high molar ratio of alcohol to acid creates novel single phase medium which overcomes mass transfer effects and facilitates downstream processing. The effect of various reaction parameters was optimized to obtain a high yield of isopropyl myristate. Effect of temperature, agitation speed, organic solvent, biocatalyst loading and batch operational stability of the enzyme was systematically studied. The conversion of 87.65% was obtained when the molar ratio of isopropyl alcohol to myristic acid (15:1) was used with 4% (w/w) catalyst loading and agitation speed of 150 rpm at 60 °C. The enzyme has also shown good batch operational stability under optimized conditions. Article in Journal/Newspaper antartic* Directory of Open Access Journals: DOAJ Articles Biotechnology Reports 8 133 137 |
spellingShingle | Isopropyl myristate Myristic acid Isopropyl alcohol Novozym 435 Esterification Biotechnology TP248.13-248.65 Rajeshkumar N. Vadgama Annamma A. Odaneth Arvind M. Lali Green synthesis of isopropyl myristate in novel single phase medium Part I: Batch optimization studies |
title | Green synthesis of isopropyl myristate in novel single phase medium Part I: Batch optimization studies |
title_full | Green synthesis of isopropyl myristate in novel single phase medium Part I: Batch optimization studies |
title_fullStr | Green synthesis of isopropyl myristate in novel single phase medium Part I: Batch optimization studies |
title_full_unstemmed | Green synthesis of isopropyl myristate in novel single phase medium Part I: Batch optimization studies |
title_short | Green synthesis of isopropyl myristate in novel single phase medium Part I: Batch optimization studies |
title_sort | green synthesis of isopropyl myristate in novel single phase medium part i: batch optimization studies |
topic | Isopropyl myristate Myristic acid Isopropyl alcohol Novozym 435 Esterification Biotechnology TP248.13-248.65 |
topic_facet | Isopropyl myristate Myristic acid Isopropyl alcohol Novozym 435 Esterification Biotechnology TP248.13-248.65 |
url | https://doi.org/10.1016/j.btre.2015.10.006 https://doaj.org/article/0eddb5e3e6bd41ca9705000349b96752 |