DETECTION OF HUMAN ANTI-ZIKA VIRUS IgG BY ELISA USING AN ANTIGEN FROM in vitro INFECTED VERO CELLS: PRELIMINARY RESULTS
SUMMARY Zika virus (ZKV) infection is a huge public health problem in Brazil because of the increased incidence of microcephaly in neonates from infected mothers. Detection of specific IgG antibodies in maternal serum samples constitutes an important approach for diagnosing ZKV infection and evaluat...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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ftdoajarticles:oai:doaj.org/article:0eb4fea3b4a147d4a9ddc0d3cb74994f 2024-09-09T19:28:28+00:00 DETECTION OF HUMAN ANTI-ZIKA VIRUS IgG BY ELISA USING AN ANTIGEN FROM in vitro INFECTED VERO CELLS: PRELIMINARY RESULTS Laura Masami SUMITA Jaqueline Polizeli RODRIGUES Noely Evangelista FERREIRA Alvina Clara FELIX Nathalia Caroline Santiago SOUZA Clarisse Martins MACHADO Heitor Franco de ANDRADE JÚNIOR https://doi.org/10.1590/s1678-9946201658089 https://doaj.org/article/0eb4fea3b4a147d4a9ddc0d3cb74994f EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652016005000270&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201658089 https://doaj.org/article/0eb4fea3b4a147d4a9ddc0d3cb74994f Revista do Instituto de Medicina Tropical de São Paulo, Vol 58, Iss 0 Zika virus Dengue virus Congenital infection IgG Serology Avidity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article ftdoajarticles https://doi.org/10.1590/s1678-9946201658089 2024-08-05T17:49:31Z SUMMARY Zika virus (ZKV) infection is a huge public health problem in Brazil because of the increased incidence of microcephaly in neonates from infected mothers. Detection of specific IgG antibodies in maternal serum samples constitutes an important approach for diagnosing ZKV infection and evaluating its relationship with neonatal microcephaly. However, as there is no serological test produced in Brazil to detect IgM and IgG antibodies against ZKV, we sought to examine specific IgG in serum samples from patients or suspected mothers to detect previous infection and to test for specificity with regard to flaviviral infections occurring in the same area. Brazilian Zika virus native antigens were obtained from infected Vero cell layers or free virions in the culture medium and then used in ELISA. We tested sera from eight ZKV RNA-diagnosed infected patients (ZKVR), seven neonates with microcephaly and their mothers after delivery (MM), 140 dengue virus IgM-positive (DM) and IgG (DG)-positive patients, and 100 yellow fever (YF)-vaccinated patients. According to the ELISA, ZKVR samples were mostly positive (7/8), and all the MM serum samples were positive for ZKV IgG (7/7). In contrast, cross-reactions for dengue or yellow fever-vaccinated patients were observed, including DM (48/95), DG (10/45) or YF (3/100) serum samples; however, these cross-reactions exhibited low antigen avidity so that 6 M urea largely removed this cross-reactivity, with only a few cross-reacting samples remaining (8/140). ELISA based on extracted virions was much more specific, with all ZKVR (8/8) and MM sera being positive for ZKV IgG (7/7) and only borderline cross-reactivity found for DM (6/95), DG (3/45) or YF (4/100)-vaccinated serum samples. This technique (ELISA) can identify specific IgG in ZKV-infected patients and may be helpful in diagnosing congenital infetions after maternal RNA virus clearance or in epidemiological studies. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 58 0 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Zika virus Dengue virus Congenital infection IgG Serology Avidity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Zika virus Dengue virus Congenital infection IgG Serology Avidity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Laura Masami SUMITA Jaqueline Polizeli RODRIGUES Noely Evangelista FERREIRA Alvina Clara FELIX Nathalia Caroline Santiago SOUZA Clarisse Martins MACHADO Heitor Franco de ANDRADE JÚNIOR DETECTION OF HUMAN ANTI-ZIKA VIRUS IgG BY ELISA USING AN ANTIGEN FROM in vitro INFECTED VERO CELLS: PRELIMINARY RESULTS |
topic_facet |
Zika virus Dengue virus Congenital infection IgG Serology Avidity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
SUMMARY Zika virus (ZKV) infection is a huge public health problem in Brazil because of the increased incidence of microcephaly in neonates from infected mothers. Detection of specific IgG antibodies in maternal serum samples constitutes an important approach for diagnosing ZKV infection and evaluating its relationship with neonatal microcephaly. However, as there is no serological test produced in Brazil to detect IgM and IgG antibodies against ZKV, we sought to examine specific IgG in serum samples from patients or suspected mothers to detect previous infection and to test for specificity with regard to flaviviral infections occurring in the same area. Brazilian Zika virus native antigens were obtained from infected Vero cell layers or free virions in the culture medium and then used in ELISA. We tested sera from eight ZKV RNA-diagnosed infected patients (ZKVR), seven neonates with microcephaly and their mothers after delivery (MM), 140 dengue virus IgM-positive (DM) and IgG (DG)-positive patients, and 100 yellow fever (YF)-vaccinated patients. According to the ELISA, ZKVR samples were mostly positive (7/8), and all the MM serum samples were positive for ZKV IgG (7/7). In contrast, cross-reactions for dengue or yellow fever-vaccinated patients were observed, including DM (48/95), DG (10/45) or YF (3/100) serum samples; however, these cross-reactions exhibited low antigen avidity so that 6 M urea largely removed this cross-reactivity, with only a few cross-reacting samples remaining (8/140). ELISA based on extracted virions was much more specific, with all ZKVR (8/8) and MM sera being positive for ZKV IgG (7/7) and only borderline cross-reactivity found for DM (6/95), DG (3/45) or YF (4/100)-vaccinated serum samples. This technique (ELISA) can identify specific IgG in ZKV-infected patients and may be helpful in diagnosing congenital infetions after maternal RNA virus clearance or in epidemiological studies. |
format |
Article in Journal/Newspaper |
author |
Laura Masami SUMITA Jaqueline Polizeli RODRIGUES Noely Evangelista FERREIRA Alvina Clara FELIX Nathalia Caroline Santiago SOUZA Clarisse Martins MACHADO Heitor Franco de ANDRADE JÚNIOR |
author_facet |
Laura Masami SUMITA Jaqueline Polizeli RODRIGUES Noely Evangelista FERREIRA Alvina Clara FELIX Nathalia Caroline Santiago SOUZA Clarisse Martins MACHADO Heitor Franco de ANDRADE JÚNIOR |
author_sort |
Laura Masami SUMITA |
title |
DETECTION OF HUMAN ANTI-ZIKA VIRUS IgG BY ELISA USING AN ANTIGEN FROM in vitro INFECTED VERO CELLS: PRELIMINARY RESULTS |
title_short |
DETECTION OF HUMAN ANTI-ZIKA VIRUS IgG BY ELISA USING AN ANTIGEN FROM in vitro INFECTED VERO CELLS: PRELIMINARY RESULTS |
title_full |
DETECTION OF HUMAN ANTI-ZIKA VIRUS IgG BY ELISA USING AN ANTIGEN FROM in vitro INFECTED VERO CELLS: PRELIMINARY RESULTS |
title_fullStr |
DETECTION OF HUMAN ANTI-ZIKA VIRUS IgG BY ELISA USING AN ANTIGEN FROM in vitro INFECTED VERO CELLS: PRELIMINARY RESULTS |
title_full_unstemmed |
DETECTION OF HUMAN ANTI-ZIKA VIRUS IgG BY ELISA USING AN ANTIGEN FROM in vitro INFECTED VERO CELLS: PRELIMINARY RESULTS |
title_sort |
detection of human anti-zika virus igg by elisa using an antigen from in vitro infected vero cells: preliminary results |
publisher |
Universidade de São Paulo (USP) |
url |
https://doi.org/10.1590/s1678-9946201658089 https://doaj.org/article/0eb4fea3b4a147d4a9ddc0d3cb74994f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 58, Iss 0 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652016005000270&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201658089 https://doaj.org/article/0eb4fea3b4a147d4a9ddc0d3cb74994f |
op_doi |
https://doi.org/10.1590/s1678-9946201658089 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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58 |
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