Immunogenic Properties of a BCG Adjuvanted Chitosan Nanoparticle-Based Dengue Vaccine in Human Dendritic Cells.
Dengue viruses (DENVs) are among the most rapidly and efficiently spreading arboviruses. WHO recently estimated that about half of the world's population is now at risk for DENV infection. There is no specific treatment or vaccine available to treat or prevent DENV infections. Here, we report t...
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ftdoajarticles:oai:doaj.org/article:0e905b18520c4cc7a6cf5b537e11ba39 2023-05-15T15:09:25+02:00 Immunogenic Properties of a BCG Adjuvanted Chitosan Nanoparticle-Based Dengue Vaccine in Human Dendritic Cells. Taweewun Hunsawong Panya Sunintaboon Saradee Warit Butsaya Thaisomboonsuk Richard G Jarman In-Kyu Yoon Sukathida Ubol Stefan Fernandez 2015-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003958 https://doaj.org/article/0e905b18520c4cc7a6cf5b537e11ba39 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4578877?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003958 https://doaj.org/article/0e905b18520c4cc7a6cf5b537e11ba39 PLoS Neglected Tropical Diseases, Vol 9, Iss 9, p e0003958 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003958 2022-12-31T16:25:01Z Dengue viruses (DENVs) are among the most rapidly and efficiently spreading arboviruses. WHO recently estimated that about half of the world's population is now at risk for DENV infection. There is no specific treatment or vaccine available to treat or prevent DENV infections. Here, we report the development of a novel dengue nanovaccine (DNV) composed of UV-inactivated DENV-2 (UVI-DENV) and Mycobacterium bovis Bacillus Calmette-Guerin cell wall components (BCG-CWCs) loaded into chitosan nanoparticles (CS-NPs). CS-NPs were prepared by an emulsion polymerization method prior to loading of the BCG-CWCs and UVI-DENV components. Using a scanning electron microscope and a zetasizer, DNV was determined to be of spherical shape with a diameter of 372.0 ± 11.2 nm in average and cationic surface properties. The loading efficacies of BCG-CWCs and UVI-DENV into the CS-NPs and BCG-CS-NPs were up to 97.2 and 98.4%, respectively. THP-1 cellular uptake of UVI-DENV present in the DNV was higher than soluble UVI-DENV alone. DNV stimulation of immature dendritic cells (iDCs) resulted in a significantly higher expression of DCs maturation markers (CD80, CD86 and HLA-DR) and induction of various cytokine and chemokine productions than in UVI-DENV-treated iDCs, suggesting a potential use of BCG- CS-NPs as adjuvant and delivery system for dengue vaccines. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Calmette ENVELOPE(-67.150,-67.150,-68.050,-68.050) PLOS Neglected Tropical Diseases 9 9 e0003958 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Taweewun Hunsawong Panya Sunintaboon Saradee Warit Butsaya Thaisomboonsuk Richard G Jarman In-Kyu Yoon Sukathida Ubol Stefan Fernandez Immunogenic Properties of a BCG Adjuvanted Chitosan Nanoparticle-Based Dengue Vaccine in Human Dendritic Cells. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Dengue viruses (DENVs) are among the most rapidly and efficiently spreading arboviruses. WHO recently estimated that about half of the world's population is now at risk for DENV infection. There is no specific treatment or vaccine available to treat or prevent DENV infections. Here, we report the development of a novel dengue nanovaccine (DNV) composed of UV-inactivated DENV-2 (UVI-DENV) and Mycobacterium bovis Bacillus Calmette-Guerin cell wall components (BCG-CWCs) loaded into chitosan nanoparticles (CS-NPs). CS-NPs were prepared by an emulsion polymerization method prior to loading of the BCG-CWCs and UVI-DENV components. Using a scanning electron microscope and a zetasizer, DNV was determined to be of spherical shape with a diameter of 372.0 ± 11.2 nm in average and cationic surface properties. The loading efficacies of BCG-CWCs and UVI-DENV into the CS-NPs and BCG-CS-NPs were up to 97.2 and 98.4%, respectively. THP-1 cellular uptake of UVI-DENV present in the DNV was higher than soluble UVI-DENV alone. DNV stimulation of immature dendritic cells (iDCs) resulted in a significantly higher expression of DCs maturation markers (CD80, CD86 and HLA-DR) and induction of various cytokine and chemokine productions than in UVI-DENV-treated iDCs, suggesting a potential use of BCG- CS-NPs as adjuvant and delivery system for dengue vaccines. |
format |
Article in Journal/Newspaper |
author |
Taweewun Hunsawong Panya Sunintaboon Saradee Warit Butsaya Thaisomboonsuk Richard G Jarman In-Kyu Yoon Sukathida Ubol Stefan Fernandez |
author_facet |
Taweewun Hunsawong Panya Sunintaboon Saradee Warit Butsaya Thaisomboonsuk Richard G Jarman In-Kyu Yoon Sukathida Ubol Stefan Fernandez |
author_sort |
Taweewun Hunsawong |
title |
Immunogenic Properties of a BCG Adjuvanted Chitosan Nanoparticle-Based Dengue Vaccine in Human Dendritic Cells. |
title_short |
Immunogenic Properties of a BCG Adjuvanted Chitosan Nanoparticle-Based Dengue Vaccine in Human Dendritic Cells. |
title_full |
Immunogenic Properties of a BCG Adjuvanted Chitosan Nanoparticle-Based Dengue Vaccine in Human Dendritic Cells. |
title_fullStr |
Immunogenic Properties of a BCG Adjuvanted Chitosan Nanoparticle-Based Dengue Vaccine in Human Dendritic Cells. |
title_full_unstemmed |
Immunogenic Properties of a BCG Adjuvanted Chitosan Nanoparticle-Based Dengue Vaccine in Human Dendritic Cells. |
title_sort |
immunogenic properties of a bcg adjuvanted chitosan nanoparticle-based dengue vaccine in human dendritic cells. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003958 https://doaj.org/article/0e905b18520c4cc7a6cf5b537e11ba39 |
long_lat |
ENVELOPE(-67.150,-67.150,-68.050,-68.050) |
geographic |
Arctic Calmette |
geographic_facet |
Arctic Calmette |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 9, p e0003958 (2015) |
op_relation |
http://europepmc.org/articles/PMC4578877?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003958 https://doaj.org/article/0e905b18520c4cc7a6cf5b537e11ba39 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003958 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
9 |
container_issue |
9 |
container_start_page |
e0003958 |
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1766340620099518464 |