Competitive endothelial adhesion between Plasmodium falciparum isolates under physiological flow conditions
Abstract Background Sequestration of parasitized red blood cells in the microvasculature of major organs involves a sequence of events that is believed to contribute to the pathogenesis of severe falciparum malaria. Plasmodium falciparum infections are commonly composed of multiple subpopulations of...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2009
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1475-2875-8-214 https://doaj.org/article/0e725ddba06b451cacdb54be2d569a9e |
id |
ftdoajarticles:oai:doaj.org/article:0e725ddba06b451cacdb54be2d569a9e |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:0e725ddba06b451cacdb54be2d569a9e 2023-05-15T15:09:23+02:00 Competitive endothelial adhesion between Plasmodium falciparum isolates under physiological flow conditions Molyneux Malcolm Montgomery Jacqui Phiri Happy Craig Alister 2009-09-01T00:00:00Z https://doi.org/10.1186/1475-2875-8-214 https://doaj.org/article/0e725ddba06b451cacdb54be2d569a9e EN eng BMC http://www.malariajournal.com/content/8/1/214 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-8-214 1475-2875 https://doaj.org/article/0e725ddba06b451cacdb54be2d569a9e Malaria Journal, Vol 8, Iss 1, p 214 (2009) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2009 ftdoajarticles https://doi.org/10.1186/1475-2875-8-214 2022-12-31T11:56:05Z Abstract Background Sequestration of parasitized red blood cells in the microvasculature of major organs involves a sequence of events that is believed to contribute to the pathogenesis of severe falciparum malaria. Plasmodium falciparum infections are commonly composed of multiple subpopulations of parasites with varied adhesive properties. A key question is: do these subpopulations compete for adhesion to endothelium? This study investigated whether, in a laboratory model of cytoadherence, there is competition in binding to endothelium between pRBC infected with P. falciparum of variant adhesive phenotypes, particularly under flow conditions. Methods Four different P. falciparum isolates, of known adherence phenotypes, were matched in pairs, mixed in different proportions and allowed to bind to cultured human endothelium. Using in vitro competitive static and flow-based adhesion assays, that allow simultaneous testing of the adhesive properties of two different parasite lines, adherence levels of paired P. falciparum isolates were quantified and analysed using either non-parametric Wilcoxon's paired signed rank test or Student paired test. Results Study findings show that P. falciparum parasite lines show marked differences in the efficiency of adhesion to endothelium. Conclusion Plasmodium falciparum variants will compete for adhesion to endothelia and variants can be ranked by their efficiency of binding. These findings suggest that variants from a mixed infection will not show uniform cytoadherence and so may vary in their ability to cause disease. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 8 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Molyneux Malcolm Montgomery Jacqui Phiri Happy Craig Alister Competitive endothelial adhesion between Plasmodium falciparum isolates under physiological flow conditions |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Sequestration of parasitized red blood cells in the microvasculature of major organs involves a sequence of events that is believed to contribute to the pathogenesis of severe falciparum malaria. Plasmodium falciparum infections are commonly composed of multiple subpopulations of parasites with varied adhesive properties. A key question is: do these subpopulations compete for adhesion to endothelium? This study investigated whether, in a laboratory model of cytoadherence, there is competition in binding to endothelium between pRBC infected with P. falciparum of variant adhesive phenotypes, particularly under flow conditions. Methods Four different P. falciparum isolates, of known adherence phenotypes, were matched in pairs, mixed in different proportions and allowed to bind to cultured human endothelium. Using in vitro competitive static and flow-based adhesion assays, that allow simultaneous testing of the adhesive properties of two different parasite lines, adherence levels of paired P. falciparum isolates were quantified and analysed using either non-parametric Wilcoxon's paired signed rank test or Student paired test. Results Study findings show that P. falciparum parasite lines show marked differences in the efficiency of adhesion to endothelium. Conclusion Plasmodium falciparum variants will compete for adhesion to endothelia and variants can be ranked by their efficiency of binding. These findings suggest that variants from a mixed infection will not show uniform cytoadherence and so may vary in their ability to cause disease. |
format |
Article in Journal/Newspaper |
author |
Molyneux Malcolm Montgomery Jacqui Phiri Happy Craig Alister |
author_facet |
Molyneux Malcolm Montgomery Jacqui Phiri Happy Craig Alister |
author_sort |
Molyneux Malcolm |
title |
Competitive endothelial adhesion between Plasmodium falciparum isolates under physiological flow conditions |
title_short |
Competitive endothelial adhesion between Plasmodium falciparum isolates under physiological flow conditions |
title_full |
Competitive endothelial adhesion between Plasmodium falciparum isolates under physiological flow conditions |
title_fullStr |
Competitive endothelial adhesion between Plasmodium falciparum isolates under physiological flow conditions |
title_full_unstemmed |
Competitive endothelial adhesion between Plasmodium falciparum isolates under physiological flow conditions |
title_sort |
competitive endothelial adhesion between plasmodium falciparum isolates under physiological flow conditions |
publisher |
BMC |
publishDate |
2009 |
url |
https://doi.org/10.1186/1475-2875-8-214 https://doaj.org/article/0e725ddba06b451cacdb54be2d569a9e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 8, Iss 1, p 214 (2009) |
op_relation |
http://www.malariajournal.com/content/8/1/214 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-8-214 1475-2875 https://doaj.org/article/0e725ddba06b451cacdb54be2d569a9e |
op_doi |
https://doi.org/10.1186/1475-2875-8-214 |
container_title |
Malaria Journal |
container_volume |
8 |
container_issue |
1 |
_version_ |
1766340589488439296 |