Production and Kinetics of Isoamyl Acetate from Acetic Anhydride using Candida Antarctica Lipase B in a Solvent-Free System
Isoamyl acetate is the most essential esters in food industry. Studies on isoamyl acetate production were expanded since 1976 through having 1174 patents that contains isoamyl acetate on that year. Most researches have used hexane or heptanes as solvent which are not preferable due to their toxic pr...
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ftdoajarticles:oai:doaj.org/article:0e6e35131a4849f9af6ecf40f748939a 2023-05-15T13:44:49+02:00 Production and Kinetics of Isoamyl Acetate from Acetic Anhydride using Candida Antarctica Lipase B in a Solvent-Free System N. Yusoff Azudin M. Mat Don S.R. Abdul Shukor 2013-06-01T00:00:00Z https://doi.org/10.3303/CET1332177 https://doaj.org/article/0e6e35131a4849f9af6ecf40f748939a EN eng AIDIC Servizi S.r.l. https://www.cetjournal.it/index.php/cet/article/view/6571 https://doaj.org/toc/2283-9216 doi:10.3303/CET1332177 2283-9216 https://doaj.org/article/0e6e35131a4849f9af6ecf40f748939a Chemical Engineering Transactions, Vol 32 (2013) Chemical engineering TP155-156 Computer engineering. Computer hardware TK7885-7895 article 2013 ftdoajarticles https://doi.org/10.3303/CET1332177 2022-12-31T15:30:56Z Isoamyl acetate is the most essential esters in food industry. Studies on isoamyl acetate production were expanded since 1976 through having 1174 patents that contains isoamyl acetate on that year. Most researches have used hexane or heptanes as solvent which are not preferable due to their toxic properties. Therefore, the risk and unfavorable conditions were removed by introducing solvent-free system. In this study, isoamyl acetate was produced using Candida Antarctica Lipase-B (CALB) by esterification reaction between acetic anhydride and isoamyl alcohol in a solvent-free system. The parameters studied were acid-alcohol molar ratio (0.1 – 2), reaction time (2-6 h), temperature (30-50 °C) and mass of enzyme (4-12 wt%). The isoamyl acetate production was maximum (41.6-fold) at acid to alcohol molar ratio of 2, mass of enzyme of 5.78 wt%, at time of 3 h and 44 °C reaction temperature. An enzymatic kinetic model based on modified Michaelis-Menten equation was developed and fitted to the non-linear regression to the experimental data. The model developed was in agreement with the experimental data with R2 value equals to 0.999. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Chemical engineering TP155-156 Computer engineering. Computer hardware TK7885-7895 |
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Chemical engineering TP155-156 Computer engineering. Computer hardware TK7885-7895 N. Yusoff Azudin M. Mat Don S.R. Abdul Shukor Production and Kinetics of Isoamyl Acetate from Acetic Anhydride using Candida Antarctica Lipase B in a Solvent-Free System |
topic_facet |
Chemical engineering TP155-156 Computer engineering. Computer hardware TK7885-7895 |
description |
Isoamyl acetate is the most essential esters in food industry. Studies on isoamyl acetate production were expanded since 1976 through having 1174 patents that contains isoamyl acetate on that year. Most researches have used hexane or heptanes as solvent which are not preferable due to their toxic properties. Therefore, the risk and unfavorable conditions were removed by introducing solvent-free system. In this study, isoamyl acetate was produced using Candida Antarctica Lipase-B (CALB) by esterification reaction between acetic anhydride and isoamyl alcohol in a solvent-free system. The parameters studied were acid-alcohol molar ratio (0.1 – 2), reaction time (2-6 h), temperature (30-50 °C) and mass of enzyme (4-12 wt%). The isoamyl acetate production was maximum (41.6-fold) at acid to alcohol molar ratio of 2, mass of enzyme of 5.78 wt%, at time of 3 h and 44 °C reaction temperature. An enzymatic kinetic model based on modified Michaelis-Menten equation was developed and fitted to the non-linear regression to the experimental data. The model developed was in agreement with the experimental data with R2 value equals to 0.999. |
format |
Article in Journal/Newspaper |
author |
N. Yusoff Azudin M. Mat Don S.R. Abdul Shukor |
author_facet |
N. Yusoff Azudin M. Mat Don S.R. Abdul Shukor |
author_sort |
N. Yusoff Azudin |
title |
Production and Kinetics of Isoamyl Acetate from Acetic Anhydride using Candida Antarctica Lipase B in a Solvent-Free System |
title_short |
Production and Kinetics of Isoamyl Acetate from Acetic Anhydride using Candida Antarctica Lipase B in a Solvent-Free System |
title_full |
Production and Kinetics of Isoamyl Acetate from Acetic Anhydride using Candida Antarctica Lipase B in a Solvent-Free System |
title_fullStr |
Production and Kinetics of Isoamyl Acetate from Acetic Anhydride using Candida Antarctica Lipase B in a Solvent-Free System |
title_full_unstemmed |
Production and Kinetics of Isoamyl Acetate from Acetic Anhydride using Candida Antarctica Lipase B in a Solvent-Free System |
title_sort |
production and kinetics of isoamyl acetate from acetic anhydride using candida antarctica lipase b in a solvent-free system |
publisher |
AIDIC Servizi S.r.l. |
publishDate |
2013 |
url |
https://doi.org/10.3303/CET1332177 https://doaj.org/article/0e6e35131a4849f9af6ecf40f748939a |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Chemical Engineering Transactions, Vol 32 (2013) |
op_relation |
https://www.cetjournal.it/index.php/cet/article/view/6571 https://doaj.org/toc/2283-9216 doi:10.3303/CET1332177 2283-9216 https://doaj.org/article/0e6e35131a4849f9af6ecf40f748939a |
op_doi |
https://doi.org/10.3303/CET1332177 |
_version_ |
1766207195000602624 |