Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth
Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins). The present study aimed to eval...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:0e6ccc99ea6c42d28954dbca35d0d8fd 2023-05-15T15:07:48+02:00 Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth A Della Torre LBL Albuquerque NM Farrapo Y Oshima-Franco MG Santos RVS Tavares ACD Rodas CA Dal Belo CRP Cardoso EA Varanda FC Groppo PS Lopes 2011-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992011000200010 https://doaj.org/article/0e6ccc99ea6c42d28954dbca35d0d8fd EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200010 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992011000200010 1678-9199 https://doaj.org/article/0e6ccc99ea6c42d28954dbca35d0d8fd Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 2, Pp 190-198 (2011) Ames test micronucleus test mutagenicity plant extract Plathymenia reticulata Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2011 ftdoajarticles https://doi.org/10.1590/S1678-91992011000200010 2022-12-31T03:40:38Z Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins). The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test) and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9) and without (-S9) metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P. reticulata extract was mutagenic to TA98 (-S9) and showed signs of mutagenic activity in TA97a and TA102 (both -S9) strains. Micronucleus test CBPI values showed that the endogenous metabolic system increased the number of viable cells when compared to the non-activated samples and the micronucleus frequency increased when the cells were treated in the absence of S9. We concluded that P. reticulata extract may present direct mutagenic properties. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 17 2 190 198 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Ames test micronucleus test mutagenicity plant extract Plathymenia reticulata Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Ames test micronucleus test mutagenicity plant extract Plathymenia reticulata Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 A Della Torre LBL Albuquerque NM Farrapo Y Oshima-Franco MG Santos RVS Tavares ACD Rodas CA Dal Belo CRP Cardoso EA Varanda FC Groppo PS Lopes Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth |
topic_facet |
Ames test micronucleus test mutagenicity plant extract Plathymenia reticulata Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins). The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test) and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9) and without (-S9) metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P. reticulata extract was mutagenic to TA98 (-S9) and showed signs of mutagenic activity in TA97a and TA102 (both -S9) strains. Micronucleus test CBPI values showed that the endogenous metabolic system increased the number of viable cells when compared to the non-activated samples and the micronucleus frequency increased when the cells were treated in the absence of S9. We concluded that P. reticulata extract may present direct mutagenic properties. |
format |
Article in Journal/Newspaper |
author |
A Della Torre LBL Albuquerque NM Farrapo Y Oshima-Franco MG Santos RVS Tavares ACD Rodas CA Dal Belo CRP Cardoso EA Varanda FC Groppo PS Lopes |
author_facet |
A Della Torre LBL Albuquerque NM Farrapo Y Oshima-Franco MG Santos RVS Tavares ACD Rodas CA Dal Belo CRP Cardoso EA Varanda FC Groppo PS Lopes |
author_sort |
A Della Torre |
title |
Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth |
title_short |
Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth |
title_full |
Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth |
title_fullStr |
Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth |
title_full_unstemmed |
Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth |
title_sort |
mutagenicity induced by the hydroalcoholic extract of the medicinal plant plathymenia reticulata benth |
publisher |
SciELO |
publishDate |
2011 |
url |
https://doi.org/10.1590/S1678-91992011000200010 https://doaj.org/article/0e6ccc99ea6c42d28954dbca35d0d8fd |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 2, Pp 190-198 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200010 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992011000200010 1678-9199 https://doaj.org/article/0e6ccc99ea6c42d28954dbca35d0d8fd |
op_doi |
https://doi.org/10.1590/S1678-91992011000200010 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
17 |
container_issue |
2 |
container_start_page |
190 |
op_container_end_page |
198 |
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1766339217098539008 |