Cloning and characterization of O-xylosyltransferase gene from Pinctada fucata martensii
Glycosaminoglycan (GAG), an important component of proteoglycan (PG), was biosynthesized with the initiation by peptide O-xylosyltransferase. O-xylosyltransferase activity was presented as a marker for increased PG synthesis. In the present study, a novel O-xylosyltransferase (OXT) gene was identifi...
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ftdoajarticles:oai:doaj.org/article:0e6c13ca2ff24f0493e394db9b469ebd 2023-05-15T15:58:44+02:00 Cloning and characterization of O-xylosyltransferase gene from Pinctada fucata martensii Ruijuan Hao Zhe Zheng Xiaodong Du Yu Jiao Yuewen Deng 2019-01-01T00:00:00Z https://doi.org/10.1080/09712119.2019.1650051 https://doaj.org/article/0e6c13ca2ff24f0493e394db9b469ebd EN eng Taylor & Francis Group http://dx.doi.org/10.1080/09712119.2019.1650051 https://doaj.org/toc/0971-2119 https://doaj.org/toc/0974-1844 0971-2119 0974-1844 doi:10.1080/09712119.2019.1650051 https://doaj.org/article/0e6c13ca2ff24f0493e394db9b469ebd Journal of Applied Animal Research, Vol 47, Iss 1, Pp 408-416 (2019) pinctada fucata martensii xylosyltransferase oxt glycosaminoglycan proteoglycans biomineralization Veterinary medicine SF600-1100 article 2019 ftdoajarticles https://doi.org/10.1080/09712119.2019.1650051 2022-12-31T11:16:01Z Glycosaminoglycan (GAG), an important component of proteoglycan (PG), was biosynthesized with the initiation by peptide O-xylosyltransferase. O-xylosyltransferase activity was presented as a marker for increased PG synthesis. In the present study, a novel O-xylosyltransferase (OXT) gene was identified from Pinctada fucata martensii (PmOXT). The PmOXT-deduced protein sequence carried a typical water-soluble carbohydrate domain, branch domain, and xylosyltransferase domain. Homologous analysis of PmOXT presented the conserved DXD motif and catalytic structure characteristics. Phylogenetic tree analysis showed the traditional taxonomy and PmOXT clustered with Crassostrea gigas. PmOXT was expressed in all the detected tissues and developmental stages. PmOXT had a significantly higher expression level in the shell formation associated tissues and developmental stages. PmOXT expressed significantly decreased in the central zone of the mantle and marginal zone of the mantle after RNA interference; additionally, OXT activity and GAG content in the extrapallial fluid were significantly reduced compared with the control. Furthermore, the crystal tablets of prismatic layer displayed obvious holes and disordered crystals with obviously rough surface and irregular crystal tablets were observed in the nacre after RNAi. Results suggested that PmOXT affected the shell formation by influencing the formation of GAGs in the process of addition to the core proteins. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Journal of Applied Animal Research 47 1 408 416 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
pinctada fucata martensii xylosyltransferase oxt glycosaminoglycan proteoglycans biomineralization Veterinary medicine SF600-1100 |
spellingShingle |
pinctada fucata martensii xylosyltransferase oxt glycosaminoglycan proteoglycans biomineralization Veterinary medicine SF600-1100 Ruijuan Hao Zhe Zheng Xiaodong Du Yu Jiao Yuewen Deng Cloning and characterization of O-xylosyltransferase gene from Pinctada fucata martensii |
topic_facet |
pinctada fucata martensii xylosyltransferase oxt glycosaminoglycan proteoglycans biomineralization Veterinary medicine SF600-1100 |
description |
Glycosaminoglycan (GAG), an important component of proteoglycan (PG), was biosynthesized with the initiation by peptide O-xylosyltransferase. O-xylosyltransferase activity was presented as a marker for increased PG synthesis. In the present study, a novel O-xylosyltransferase (OXT) gene was identified from Pinctada fucata martensii (PmOXT). The PmOXT-deduced protein sequence carried a typical water-soluble carbohydrate domain, branch domain, and xylosyltransferase domain. Homologous analysis of PmOXT presented the conserved DXD motif and catalytic structure characteristics. Phylogenetic tree analysis showed the traditional taxonomy and PmOXT clustered with Crassostrea gigas. PmOXT was expressed in all the detected tissues and developmental stages. PmOXT had a significantly higher expression level in the shell formation associated tissues and developmental stages. PmOXT expressed significantly decreased in the central zone of the mantle and marginal zone of the mantle after RNA interference; additionally, OXT activity and GAG content in the extrapallial fluid were significantly reduced compared with the control. Furthermore, the crystal tablets of prismatic layer displayed obvious holes and disordered crystals with obviously rough surface and irregular crystal tablets were observed in the nacre after RNAi. Results suggested that PmOXT affected the shell formation by influencing the formation of GAGs in the process of addition to the core proteins. |
format |
Article in Journal/Newspaper |
author |
Ruijuan Hao Zhe Zheng Xiaodong Du Yu Jiao Yuewen Deng |
author_facet |
Ruijuan Hao Zhe Zheng Xiaodong Du Yu Jiao Yuewen Deng |
author_sort |
Ruijuan Hao |
title |
Cloning and characterization of O-xylosyltransferase gene from Pinctada fucata martensii |
title_short |
Cloning and characterization of O-xylosyltransferase gene from Pinctada fucata martensii |
title_full |
Cloning and characterization of O-xylosyltransferase gene from Pinctada fucata martensii |
title_fullStr |
Cloning and characterization of O-xylosyltransferase gene from Pinctada fucata martensii |
title_full_unstemmed |
Cloning and characterization of O-xylosyltransferase gene from Pinctada fucata martensii |
title_sort |
cloning and characterization of o-xylosyltransferase gene from pinctada fucata martensii |
publisher |
Taylor & Francis Group |
publishDate |
2019 |
url |
https://doi.org/10.1080/09712119.2019.1650051 https://doaj.org/article/0e6c13ca2ff24f0493e394db9b469ebd |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Journal of Applied Animal Research, Vol 47, Iss 1, Pp 408-416 (2019) |
op_relation |
http://dx.doi.org/10.1080/09712119.2019.1650051 https://doaj.org/toc/0971-2119 https://doaj.org/toc/0974-1844 0971-2119 0974-1844 doi:10.1080/09712119.2019.1650051 https://doaj.org/article/0e6c13ca2ff24f0493e394db9b469ebd |
op_doi |
https://doi.org/10.1080/09712119.2019.1650051 |
container_title |
Journal of Applied Animal Research |
container_volume |
47 |
container_issue |
1 |
container_start_page |
408 |
op_container_end_page |
416 |
_version_ |
1766394507851464704 |