Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi
Abstract Background The zoonotic simian parasite Plasmodium cynomolgi develops into replicating schizonts and dormant hypnozoites during the infection of hepatocytes and is used as a model organism to study relapsing malaria. The transcriptional profiling of P. cynomolgi liver stages was previously...
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ftdoajarticles:oai:doaj.org/article:0e680a8fe8ac407888ecdc3feea9dfd8 2023-05-15T15:07:36+02:00 Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi Gabriel Mitchell Guglielmo Roma Annemarie Voorberg-van der Wel Martin Beibel Anne-Marie Zeeman Sven Schuierer Laura Torres Erika L. Flannery Clemens H. M. Kocken Sebastian A. Mikolajczak Thierry T. Diagana 2022-12-01T00:00:00Z https://doi.org/10.1186/s12936-022-04411-3 https://doaj.org/article/0e680a8fe8ac407888ecdc3feea9dfd8 EN eng BMC https://doi.org/10.1186/s12936-022-04411-3 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-022-04411-3 1475-2875 https://doaj.org/article/0e680a8fe8ac407888ecdc3feea9dfd8 Malaria Journal, Vol 21, Iss 1, Pp 1-12 (2022) Host response Relapsing malaria Schizonts Hypnozoites RNA sequencing Liver cells Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2022 ftdoajarticles https://doi.org/10.1186/s12936-022-04411-3 2022-12-30T19:28:17Z Abstract Background The zoonotic simian parasite Plasmodium cynomolgi develops into replicating schizonts and dormant hypnozoites during the infection of hepatocytes and is used as a model organism to study relapsing malaria. The transcriptional profiling of P. cynomolgi liver stages was previously reported and revealed many important biological features of the parasite but left out the host response to malaria infection. Methods Previously published RNA sequencing data were used to quantify the expression of host genes in rhesus macaque hepatocytes infected with P . cynomolgi in comparison to either cells from uninfected samples or uninfected bystander cells. Results Although the dataset could not be used to resolve the transcriptional profile of hypnozoite-infected hepatocytes, it provided a snapshot of the host response to liver stage schizonts at 9–10 day post-infection and identified specific host pathways that are modulated during the exo-erythrocytic stage of P. cynomolgi. Conclusions This study constitutes a valuable resource characterizing the hepatocyte response to P. cynomolgi infection and provides a framework to build on future research that aims at understanding hepatocyte-parasite interactions during relapsing malaria infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 21 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Host response Relapsing malaria Schizonts Hypnozoites RNA sequencing Liver cells Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Host response Relapsing malaria Schizonts Hypnozoites RNA sequencing Liver cells Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Gabriel Mitchell Guglielmo Roma Annemarie Voorberg-van der Wel Martin Beibel Anne-Marie Zeeman Sven Schuierer Laura Torres Erika L. Flannery Clemens H. M. Kocken Sebastian A. Mikolajczak Thierry T. Diagana Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi |
topic_facet |
Host response Relapsing malaria Schizonts Hypnozoites RNA sequencing Liver cells Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The zoonotic simian parasite Plasmodium cynomolgi develops into replicating schizonts and dormant hypnozoites during the infection of hepatocytes and is used as a model organism to study relapsing malaria. The transcriptional profiling of P. cynomolgi liver stages was previously reported and revealed many important biological features of the parasite but left out the host response to malaria infection. Methods Previously published RNA sequencing data were used to quantify the expression of host genes in rhesus macaque hepatocytes infected with P . cynomolgi in comparison to either cells from uninfected samples or uninfected bystander cells. Results Although the dataset could not be used to resolve the transcriptional profile of hypnozoite-infected hepatocytes, it provided a snapshot of the host response to liver stage schizonts at 9–10 day post-infection and identified specific host pathways that are modulated during the exo-erythrocytic stage of P. cynomolgi. Conclusions This study constitutes a valuable resource characterizing the hepatocyte response to P. cynomolgi infection and provides a framework to build on future research that aims at understanding hepatocyte-parasite interactions during relapsing malaria infection. |
format |
Article in Journal/Newspaper |
author |
Gabriel Mitchell Guglielmo Roma Annemarie Voorberg-van der Wel Martin Beibel Anne-Marie Zeeman Sven Schuierer Laura Torres Erika L. Flannery Clemens H. M. Kocken Sebastian A. Mikolajczak Thierry T. Diagana |
author_facet |
Gabriel Mitchell Guglielmo Roma Annemarie Voorberg-van der Wel Martin Beibel Anne-Marie Zeeman Sven Schuierer Laura Torres Erika L. Flannery Clemens H. M. Kocken Sebastian A. Mikolajczak Thierry T. Diagana |
author_sort |
Gabriel Mitchell |
title |
Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi |
title_short |
Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi |
title_full |
Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi |
title_fullStr |
Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi |
title_full_unstemmed |
Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi |
title_sort |
transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite plasmodium cynomolgi |
publisher |
BMC |
publishDate |
2022 |
url |
https://doi.org/10.1186/s12936-022-04411-3 https://doaj.org/article/0e680a8fe8ac407888ecdc3feea9dfd8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 21, Iss 1, Pp 1-12 (2022) |
op_relation |
https://doi.org/10.1186/s12936-022-04411-3 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-022-04411-3 1475-2875 https://doaj.org/article/0e680a8fe8ac407888ecdc3feea9dfd8 |
op_doi |
https://doi.org/10.1186/s12936-022-04411-3 |
container_title |
Malaria Journal |
container_volume |
21 |
container_issue |
1 |
_version_ |
1766339072103546880 |