Production of cytokine and chemokines by human mononuclear cells and whole blood cells after infection with Trypanosoma cruzi
INTRODUCTION: The innate immune response is the first mechanism of protection against Trypanosoma cruzi, and the interaction of inflammatory cells with parasite molecules may activate this response and modulate the adaptive immune system. This study aimed to analyze the levels of cytokines and chemo...
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ftdoajarticles:oai:doaj.org/article:0e33d984206542f0b8e41b8a9dc8e528 2023-05-15T15:09:27+02:00 Production of cytokine and chemokines by human mononuclear cells and whole blood cells after infection with Trypanosoma cruzi Karine Rezende-Oliveira Ronaldo Rodrigues Sarmento Virmondes Rodrigues Junior 2012-02-01T00:00:00Z https://doi.org/10.1590/S0037-86822012000100009 https://doaj.org/article/0e33d984206542f0b8e41b8a9dc8e528 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000100009&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000100009 https://doaj.org/article/0e33d984206542f0b8e41b8a9dc8e528 Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 1, Pp 45-50 (2012) Trypanosoma cruzi Imunidade inata Citocinas Quimiocinas Arctic medicine. Tropical medicine RC955-962 article 2012 ftdoajarticles https://doi.org/10.1590/S0037-86822012000100009 2022-12-30T21:37:16Z INTRODUCTION: The innate immune response is the first mechanism of protection against Trypanosoma cruzi, and the interaction of inflammatory cells with parasite molecules may activate this response and modulate the adaptive immune system. This study aimed to analyze the levels of cytokines and chemokines synthesized by the whole blood cells (WBC) and peripheral blood mononuclear cells (PBMC) of individuals seronegative for Chagas disease after interaction with live T. cruzi trypomastigotes. METHODS: IL-12, IL-10, TNF-α, TGF-β, CCL-5, CCL-2, CCL-3, and CXCL-9 were measured by ELISA. Nitrite was determined by the Griess method. RESULTS: IL-10 was produced at high levels by WBC compared with PBMC, even after incubation with live trypomastigotes. Production of TNF-α by both PBMC and WBC was significantly higher after stimulation with trypomastigotes. Only PBMC produced significantly higher levels of IL-12 after parasite stimulation. Stimulation of cultures with trypomastigotes induced an increase of CXCL-9 levels produced by WBC. Nitrite levels produced by PBMC increased after the addition of parasites to the culture. CONCLUSIONS: Surface molecules of T. cruzi may induce the production of cytokines and chemokines by cells of the innate immune system through the activation of specific receptors not evaluated in this experiment. The ability to induce IL-12 and TNF-α contributes to shift the adaptive response towards a Th1 profile. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 45 1 45 50 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Trypanosoma cruzi Imunidade inata Citocinas Quimiocinas Arctic medicine. Tropical medicine RC955-962 |
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Trypanosoma cruzi Imunidade inata Citocinas Quimiocinas Arctic medicine. Tropical medicine RC955-962 Karine Rezende-Oliveira Ronaldo Rodrigues Sarmento Virmondes Rodrigues Junior Production of cytokine and chemokines by human mononuclear cells and whole blood cells after infection with Trypanosoma cruzi |
topic_facet |
Trypanosoma cruzi Imunidade inata Citocinas Quimiocinas Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: The innate immune response is the first mechanism of protection against Trypanosoma cruzi, and the interaction of inflammatory cells with parasite molecules may activate this response and modulate the adaptive immune system. This study aimed to analyze the levels of cytokines and chemokines synthesized by the whole blood cells (WBC) and peripheral blood mononuclear cells (PBMC) of individuals seronegative for Chagas disease after interaction with live T. cruzi trypomastigotes. METHODS: IL-12, IL-10, TNF-α, TGF-β, CCL-5, CCL-2, CCL-3, and CXCL-9 were measured by ELISA. Nitrite was determined by the Griess method. RESULTS: IL-10 was produced at high levels by WBC compared with PBMC, even after incubation with live trypomastigotes. Production of TNF-α by both PBMC and WBC was significantly higher after stimulation with trypomastigotes. Only PBMC produced significantly higher levels of IL-12 after parasite stimulation. Stimulation of cultures with trypomastigotes induced an increase of CXCL-9 levels produced by WBC. Nitrite levels produced by PBMC increased after the addition of parasites to the culture. CONCLUSIONS: Surface molecules of T. cruzi may induce the production of cytokines and chemokines by cells of the innate immune system through the activation of specific receptors not evaluated in this experiment. The ability to induce IL-12 and TNF-α contributes to shift the adaptive response towards a Th1 profile. |
format |
Article in Journal/Newspaper |
author |
Karine Rezende-Oliveira Ronaldo Rodrigues Sarmento Virmondes Rodrigues Junior |
author_facet |
Karine Rezende-Oliveira Ronaldo Rodrigues Sarmento Virmondes Rodrigues Junior |
author_sort |
Karine Rezende-Oliveira |
title |
Production of cytokine and chemokines by human mononuclear cells and whole blood cells after infection with Trypanosoma cruzi |
title_short |
Production of cytokine and chemokines by human mononuclear cells and whole blood cells after infection with Trypanosoma cruzi |
title_full |
Production of cytokine and chemokines by human mononuclear cells and whole blood cells after infection with Trypanosoma cruzi |
title_fullStr |
Production of cytokine and chemokines by human mononuclear cells and whole blood cells after infection with Trypanosoma cruzi |
title_full_unstemmed |
Production of cytokine and chemokines by human mononuclear cells and whole blood cells after infection with Trypanosoma cruzi |
title_sort |
production of cytokine and chemokines by human mononuclear cells and whole blood cells after infection with trypanosoma cruzi |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2012 |
url |
https://doi.org/10.1590/S0037-86822012000100009 https://doaj.org/article/0e33d984206542f0b8e41b8a9dc8e528 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 1, Pp 45-50 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000100009&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000100009 https://doaj.org/article/0e33d984206542f0b8e41b8a9dc8e528 |
op_doi |
https://doi.org/10.1590/S0037-86822012000100009 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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45 |
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1 |
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45 |
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50 |
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1766340650558554112 |