Prevalence of Wēnzhōu virus in small mammals in Yunnan Province, China.
BACKGROUND:Mammarenaviruses are associated with human hemorrhagic fever diseases in Africa and America. Recently, a rodent mammarenavirus, Wēnzhōu virus (WENV) and related viruses, have been reported in China, Cambodia, and Thailand. Moreover, in Cambodia, these viruses were suspected to be associat...
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ftdoajarticles:oai:doaj.org/article:0dc815d159ae4372aad649017bb49683 2023-05-15T15:11:23+02:00 Prevalence of Wēnzhōu virus in small mammals in Yunnan Province, China. Jinxia Wang Xinglou Yang Haizhou Liu Li Wang Jihua Zhou Xi Han Yan Zhu Weihong Yang Hong Pan Yunzhi Zhang Zhengli Shi 2019-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007049 https://doaj.org/article/0dc815d159ae4372aad649017bb49683 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6395006?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007049 https://doaj.org/article/0dc815d159ae4372aad649017bb49683 PLoS Neglected Tropical Diseases, Vol 13, Iss 2, p e0007049 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007049 2022-12-31T03:41:33Z BACKGROUND:Mammarenaviruses are associated with human hemorrhagic fever diseases in Africa and America. Recently, a rodent mammarenavirus, Wēnzhōu virus (WENV) and related viruses, have been reported in China, Cambodia, and Thailand. Moreover, in Cambodia, these viruses were suspected to be associated with human disease. In China, Yunnan Province is famous for its abundant animal and plant diversity and is adjacent to several South-eastern Asia countries. Therefore, it is necessary to know whether WENV-related viruses, or other mammarenaviruses, are prevalent in this province. METHODOLOGY/PRINCIPAL FINDINGS:Small mammals were trapped, euthanized, and sampled. Mammarenavirus RNA was detected using a nested reverse transcription polymerase chain reaction (RT-PCR) and quantified by real-time RT-PCR. A total of 1040 small mammals belonging to 13 genera and 26 species were trapped in Yunnan Province. WENV-related mammarenaviruses were detected in 41 rodent liver samples, mainly in brown rats (Rattus norvegicus) and oriental house rats (R. tanezumi).Viral nucleocapsid protein was detected in liver sections by indirect immunofluorescence assay. Full-length-genomes were amplified by RT-PCR and used for phylogenetic analysis with the MEGA package. Recombination analysis was performed using the SimPlot and Recombination Detection Program. CONCLUSIONS/SIGNIFICANCE:WENV related viruses circulated in small mammals in Yunnan Province. Whole genome sequence analysis of five selected viral strains showed that these viruses are closely related to WENVs discovered in Asia and form an independent branch in the phylogenetic tree in the WENV clade. Paying attention to investigate the influence of these viruses to public health is essential in the epidemic regions. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 2 e0007049 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jinxia Wang Xinglou Yang Haizhou Liu Li Wang Jihua Zhou Xi Han Yan Zhu Weihong Yang Hong Pan Yunzhi Zhang Zhengli Shi Prevalence of Wēnzhōu virus in small mammals in Yunnan Province, China. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Mammarenaviruses are associated with human hemorrhagic fever diseases in Africa and America. Recently, a rodent mammarenavirus, Wēnzhōu virus (WENV) and related viruses, have been reported in China, Cambodia, and Thailand. Moreover, in Cambodia, these viruses were suspected to be associated with human disease. In China, Yunnan Province is famous for its abundant animal and plant diversity and is adjacent to several South-eastern Asia countries. Therefore, it is necessary to know whether WENV-related viruses, or other mammarenaviruses, are prevalent in this province. METHODOLOGY/PRINCIPAL FINDINGS:Small mammals were trapped, euthanized, and sampled. Mammarenavirus RNA was detected using a nested reverse transcription polymerase chain reaction (RT-PCR) and quantified by real-time RT-PCR. A total of 1040 small mammals belonging to 13 genera and 26 species were trapped in Yunnan Province. WENV-related mammarenaviruses were detected in 41 rodent liver samples, mainly in brown rats (Rattus norvegicus) and oriental house rats (R. tanezumi).Viral nucleocapsid protein was detected in liver sections by indirect immunofluorescence assay. Full-length-genomes were amplified by RT-PCR and used for phylogenetic analysis with the MEGA package. Recombination analysis was performed using the SimPlot and Recombination Detection Program. CONCLUSIONS/SIGNIFICANCE:WENV related viruses circulated in small mammals in Yunnan Province. Whole genome sequence analysis of five selected viral strains showed that these viruses are closely related to WENVs discovered in Asia and form an independent branch in the phylogenetic tree in the WENV clade. Paying attention to investigate the influence of these viruses to public health is essential in the epidemic regions. |
format |
Article in Journal/Newspaper |
author |
Jinxia Wang Xinglou Yang Haizhou Liu Li Wang Jihua Zhou Xi Han Yan Zhu Weihong Yang Hong Pan Yunzhi Zhang Zhengli Shi |
author_facet |
Jinxia Wang Xinglou Yang Haizhou Liu Li Wang Jihua Zhou Xi Han Yan Zhu Weihong Yang Hong Pan Yunzhi Zhang Zhengli Shi |
author_sort |
Jinxia Wang |
title |
Prevalence of Wēnzhōu virus in small mammals in Yunnan Province, China. |
title_short |
Prevalence of Wēnzhōu virus in small mammals in Yunnan Province, China. |
title_full |
Prevalence of Wēnzhōu virus in small mammals in Yunnan Province, China. |
title_fullStr |
Prevalence of Wēnzhōu virus in small mammals in Yunnan Province, China. |
title_full_unstemmed |
Prevalence of Wēnzhōu virus in small mammals in Yunnan Province, China. |
title_sort |
prevalence of wēnzhōu virus in small mammals in yunnan province, china. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007049 https://doaj.org/article/0dc815d159ae4372aad649017bb49683 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 2, p e0007049 (2019) |
op_relation |
http://europepmc.org/articles/PMC6395006?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007049 https://doaj.org/article/0dc815d159ae4372aad649017bb49683 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007049 |
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PLOS Neglected Tropical Diseases |
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13 |
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2 |
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e0007049 |
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