Evaluation of the use of real-time PCR for human T cell lymphotropic virus 1 and 2 as a confirmatory test in screening for blood donors Análise do uso da PCR em tempo real para HTLV-1 e 2 como teste confirmatório na triagem de doadores de sangue

• Published in: Revista da Sociedade Brasileira de Medicina Tropical
• Main Authors: Rafaela Gomes Andrade, Maísa Aparecida Ribeiro, Maria Sueli Silva Namen-Lopes, Sônia Mara Nunes Silva, Fernando Valadares Basques, João Gabriel Ribas, Anna Bárbara de Freitas Carneiro-Proietti, Marina Lobato Martins
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2010
• Subjects: HTLV; Doadores de sangue; PCR em tempo real; Western blot; Blood donors; Real-time PCR; Arctic medicine. Tropical medicine; RC955-962; Alta; Arctic; Eia; Elevado
• Online Access: https://doi.org/10.1590/S0037-86822010000200001; https://doaj.org/article/0d8f676457774454baa335ed78d88739

INTRODUCTION: HTLV-1/2 screening among blood donors commonly utilizes an enzyme-linked immunosorbent assay (EIA), followed by a confirmatory method such as Western blot (WB) if the EIA is positive. However, this algorithm yields a high rate of inconclusive results, and is expensive. METHODS: Two qua...