Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region
Abstract Background Different antigens are needed to characterize Plasmodium falciparum infection in terms of seroreactivity and targets for invasion inhibition, in order to guide and identify the proper use of such proteins as tools for the development of serological markers and/or as vaccine candi...
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ftdoajarticles:oai:doaj.org/article:0d549ebaa7bd4cea8377e8b56352dcf1 2023-05-15T15:14:45+02:00 Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region Jorge Bendezu Elizabeth Villasis Sandra Morales Ruiz Katherine Garro Berónica Infante Renzo Gutierrez-Loli Pamela Rodríguez Manolo Fernández-Díaz Dionicia Gamboa Katherine Torres 2019-09-01T00:00:00Z https://doi.org/10.1186/s12936-019-2959-8 https://doaj.org/article/0d549ebaa7bd4cea8377e8b56352dcf1 EN eng BMC http://link.springer.com/article/10.1186/s12936-019-2959-8 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2959-8 1475-2875 https://doaj.org/article/0d549ebaa7bd4cea8377e8b56352dcf1 Malaria Journal, Vol 18, Iss 1, Pp 1-13 (2019) Plasmodium falciparum PfMSP10 Monoclonal antibodies Peptides Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2019 ftdoajarticles https://doi.org/10.1186/s12936-019-2959-8 2022-12-31T03:27:55Z Abstract Background Different antigens are needed to characterize Plasmodium falciparum infection in terms of seroreactivity and targets for invasion inhibition, in order to guide and identify the proper use of such proteins as tools for the development of serological markers and/or as vaccine candidates. Methods IgG responses in 84 serum samples from individuals with P. falciparum infection [classified as symptomatic (Sym) or asymptomatic (Asym)], or acute Plasmodium vivax infection, from the Peruvian Amazon region, were evaluated by enzyme-linked immunosorbent assays specific for a baculovirus-produced recombinant protein P. falciparum Merozoite Surface Protein 10 (rMSP10) and for non-EGF region selected peptides of PfMSP10 selected by a bioinformatics tool (PfMSP10-1, PfMSP10-2 and PfMSP10-3). Monoclonal antibodies against the selected peptides were evaluated by western blotting, confocal microscopy and inhibition invasion assays. Results Seroreactivity analysis of the P. falciparum Sym- and Asym-infected individuals against rMSP10 showed a higher response as compared to the individuals with P. vivax acute infection. IgG responses against peptide PfMSP10-1 were weak. Interestingly high IgG response was found against peptide PfMSP10-2 and the combination of peptides PfMSP10-1 + PfMSP10-2. Monoclonal antibodies were capable of detecting native PfMSP10 on purified schizonts by western blot and confocal microscopy. A low percentage of inhibition of merozoite invasion of erythrocytes in vitro was observed when the monoclonal antibodies were compared with the control antibody against AMA-1 antigen. Further studies are needed to evaluate the role of PfMSP10 in the merozoite invasion. Conclusions The rMSP10 and the PfMSP10-2 peptide synthesized for this study may be useful antigens for evaluation of P. falciparum malaria exposure in Sym and Asym individuals from the Peruvian Amazon region. Moreover, these antigens can be used for further investigation of the role of this protein in other malaria-endemic areas. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 18 1 |
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English |
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Plasmodium falciparum PfMSP10 Monoclonal antibodies Peptides Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Plasmodium falciparum PfMSP10 Monoclonal antibodies Peptides Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Jorge Bendezu Elizabeth Villasis Sandra Morales Ruiz Katherine Garro Berónica Infante Renzo Gutierrez-Loli Pamela Rodríguez Manolo Fernández-Díaz Dionicia Gamboa Katherine Torres Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region |
topic_facet |
Plasmodium falciparum PfMSP10 Monoclonal antibodies Peptides Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Different antigens are needed to characterize Plasmodium falciparum infection in terms of seroreactivity and targets for invasion inhibition, in order to guide and identify the proper use of such proteins as tools for the development of serological markers and/or as vaccine candidates. Methods IgG responses in 84 serum samples from individuals with P. falciparum infection [classified as symptomatic (Sym) or asymptomatic (Asym)], or acute Plasmodium vivax infection, from the Peruvian Amazon region, were evaluated by enzyme-linked immunosorbent assays specific for a baculovirus-produced recombinant protein P. falciparum Merozoite Surface Protein 10 (rMSP10) and for non-EGF region selected peptides of PfMSP10 selected by a bioinformatics tool (PfMSP10-1, PfMSP10-2 and PfMSP10-3). Monoclonal antibodies against the selected peptides were evaluated by western blotting, confocal microscopy and inhibition invasion assays. Results Seroreactivity analysis of the P. falciparum Sym- and Asym-infected individuals against rMSP10 showed a higher response as compared to the individuals with P. vivax acute infection. IgG responses against peptide PfMSP10-1 were weak. Interestingly high IgG response was found against peptide PfMSP10-2 and the combination of peptides PfMSP10-1 + PfMSP10-2. Monoclonal antibodies were capable of detecting native PfMSP10 on purified schizonts by western blot and confocal microscopy. A low percentage of inhibition of merozoite invasion of erythrocytes in vitro was observed when the monoclonal antibodies were compared with the control antibody against AMA-1 antigen. Further studies are needed to evaluate the role of PfMSP10 in the merozoite invasion. Conclusions The rMSP10 and the PfMSP10-2 peptide synthesized for this study may be useful antigens for evaluation of P. falciparum malaria exposure in Sym and Asym individuals from the Peruvian Amazon region. Moreover, these antigens can be used for further investigation of the role of this protein in other malaria-endemic areas. |
format |
Article in Journal/Newspaper |
author |
Jorge Bendezu Elizabeth Villasis Sandra Morales Ruiz Katherine Garro Berónica Infante Renzo Gutierrez-Loli Pamela Rodríguez Manolo Fernández-Díaz Dionicia Gamboa Katherine Torres |
author_facet |
Jorge Bendezu Elizabeth Villasis Sandra Morales Ruiz Katherine Garro Berónica Infante Renzo Gutierrez-Loli Pamela Rodríguez Manolo Fernández-Díaz Dionicia Gamboa Katherine Torres |
author_sort |
Jorge Bendezu |
title |
Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region |
title_short |
Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region |
title_full |
Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region |
title_fullStr |
Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region |
title_full_unstemmed |
Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region |
title_sort |
evaluation of plasmodium falciparum msp10 and its development as a serological tool for the peruvian amazon region |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12936-019-2959-8 https://doaj.org/article/0d549ebaa7bd4cea8377e8b56352dcf1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 18, Iss 1, Pp 1-13 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s12936-019-2959-8 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2959-8 1475-2875 https://doaj.org/article/0d549ebaa7bd4cea8377e8b56352dcf1 |
op_doi |
https://doi.org/10.1186/s12936-019-2959-8 |
container_title |
Malaria Journal |
container_volume |
18 |
container_issue |
1 |
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1766345168617734144 |