Copepod-Associated Gammaproteobacterial Alkaline Phosphatases in the North Atlantic Subtropical Gyre
Planktonic organisms may provide a niche to associated bacteria in the oligotrophic ocean. Bacterial fitness strategies in association with copepods – abundant planktonic crustaceans – were examined by sampling and incubation experiments in the North Atlantic Subtropical Gyre (NASG). The bacterial m...
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ftdoajarticles:oai:doaj.org/article:0d300c02a8d5495d88a23ebde4471713 2023-05-15T17:28:21+02:00 Copepod-Associated Gammaproteobacterial Alkaline Phosphatases in the North Atlantic Subtropical Gyre Katyanne M. Shoemaker Elizabeth A. McCliment Pia H. Moisander 2020-05-01T00:00:00Z https://doi.org/10.3389/fmicb.2020.01033 https://doaj.org/article/0d300c02a8d5495d88a23ebde4471713 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fmicb.2020.01033/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2020.01033 https://doaj.org/article/0d300c02a8d5495d88a23ebde4471713 Frontiers in Microbiology, Vol 11 (2020) metatranscriptome copepod microbiome zoosphere alkaline phophatase gammaproteobacteria Vibrio Microbiology QR1-502 article 2020 ftdoajarticles https://doi.org/10.3389/fmicb.2020.01033 2022-12-31T11:39:27Z Planktonic organisms may provide a niche to associated bacteria in the oligotrophic ocean. Bacterial fitness strategies in association with copepods – abundant planktonic crustaceans – were examined by sampling and incubation experiments in the North Atlantic Subtropical Gyre (NASG). The bacterial metatranscriptome was dominated by Gammaproteobacteria and showed expression of complete bacterial pathways including chemotaxis, cell signaling, and alkaline phosphatase activity. Quantitative PCR and reverse transcriptase qPCR revealed the consistent presence and expression of alkaline phosphatase genes primarily by Vibrio spp. in the copepod association. Copepod-associated bacteria appear to respond to prevailing phosphorus limitation by using alkaline phosphatases to break down organophosphoesters, presumably originating from the copepods. The results suggest that the basin-wide tendency for phosphorus limitation in the North Atlantic Ocean is occurring at microscales in these nitrogen-enriched copepod microenvironments. The bacterial communities and their fitness strategies supported by associations with these abundant mesozooplankton are unique from the surrounding seawater and could have large-scale implications for biogeochemical cycling, marine food web structuring, and copepod and ecosystem health. Article in Journal/Newspaper North Atlantic Copepods Directory of Open Access Journals: DOAJ Articles Frontiers in Microbiology 11 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
metatranscriptome copepod microbiome zoosphere alkaline phophatase gammaproteobacteria Vibrio Microbiology QR1-502 |
spellingShingle |
metatranscriptome copepod microbiome zoosphere alkaline phophatase gammaproteobacteria Vibrio Microbiology QR1-502 Katyanne M. Shoemaker Elizabeth A. McCliment Pia H. Moisander Copepod-Associated Gammaproteobacterial Alkaline Phosphatases in the North Atlantic Subtropical Gyre |
topic_facet |
metatranscriptome copepod microbiome zoosphere alkaline phophatase gammaproteobacteria Vibrio Microbiology QR1-502 |
description |
Planktonic organisms may provide a niche to associated bacteria in the oligotrophic ocean. Bacterial fitness strategies in association with copepods – abundant planktonic crustaceans – were examined by sampling and incubation experiments in the North Atlantic Subtropical Gyre (NASG). The bacterial metatranscriptome was dominated by Gammaproteobacteria and showed expression of complete bacterial pathways including chemotaxis, cell signaling, and alkaline phosphatase activity. Quantitative PCR and reverse transcriptase qPCR revealed the consistent presence and expression of alkaline phosphatase genes primarily by Vibrio spp. in the copepod association. Copepod-associated bacteria appear to respond to prevailing phosphorus limitation by using alkaline phosphatases to break down organophosphoesters, presumably originating from the copepods. The results suggest that the basin-wide tendency for phosphorus limitation in the North Atlantic Ocean is occurring at microscales in these nitrogen-enriched copepod microenvironments. The bacterial communities and their fitness strategies supported by associations with these abundant mesozooplankton are unique from the surrounding seawater and could have large-scale implications for biogeochemical cycling, marine food web structuring, and copepod and ecosystem health. |
format |
Article in Journal/Newspaper |
author |
Katyanne M. Shoemaker Elizabeth A. McCliment Pia H. Moisander |
author_facet |
Katyanne M. Shoemaker Elizabeth A. McCliment Pia H. Moisander |
author_sort |
Katyanne M. Shoemaker |
title |
Copepod-Associated Gammaproteobacterial Alkaline Phosphatases in the North Atlantic Subtropical Gyre |
title_short |
Copepod-Associated Gammaproteobacterial Alkaline Phosphatases in the North Atlantic Subtropical Gyre |
title_full |
Copepod-Associated Gammaproteobacterial Alkaline Phosphatases in the North Atlantic Subtropical Gyre |
title_fullStr |
Copepod-Associated Gammaproteobacterial Alkaline Phosphatases in the North Atlantic Subtropical Gyre |
title_full_unstemmed |
Copepod-Associated Gammaproteobacterial Alkaline Phosphatases in the North Atlantic Subtropical Gyre |
title_sort |
copepod-associated gammaproteobacterial alkaline phosphatases in the north atlantic subtropical gyre |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmicb.2020.01033 https://doaj.org/article/0d300c02a8d5495d88a23ebde4471713 |
genre |
North Atlantic Copepods |
genre_facet |
North Atlantic Copepods |
op_source |
Frontiers in Microbiology, Vol 11 (2020) |
op_relation |
https://www.frontiersin.org/article/10.3389/fmicb.2020.01033/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2020.01033 https://doaj.org/article/0d300c02a8d5495d88a23ebde4471713 |
op_doi |
https://doi.org/10.3389/fmicb.2020.01033 |
container_title |
Frontiers in Microbiology |
container_volume |
11 |
_version_ |
1766120971337465856 |