Screening of phytocompounds, molecular docking studies, and in vivo anti-inflammatory activity of heartwood aqueous extract of Pterocarpus santalinus L.f.
Objective: To evaluate the anti-inflammatory potential of aqueous extract of Pterocarpus santalinus L.f. heartwood using molecular docking and in vivo experiment. Methods: An aqueous extract of Pterocarpus santalinus heartwood was prepared using a Soxhlet apparatus. Phytocompounds in the extract wer...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2021
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Online Access: | https://doi.org/10.4103/2221-1691.303604 https://doaj.org/article/0cdc6a6c8f844244ad5a9ca3193ff73b |
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ftdoajarticles:oai:doaj.org/article:0cdc6a6c8f844244ad5a9ca3193ff73b 2023-05-15T15:08:44+02:00 Screening of phytocompounds, molecular docking studies, and in vivo anti-inflammatory activity of heartwood aqueous extract of Pterocarpus santalinus L.f. C N Shanti Vasudevan Bibu John Kariyil D Athira Nair I'ma Neerakkal 2021-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.303604 https://doaj.org/article/0cdc6a6c8f844244ad5a9ca3193ff73b EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=2;spage=59;epage=65;aulast=Vasudevan https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.303604 https://doaj.org/article/0cdc6a6c8f844244ad5a9ca3193ff73b Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 2, Pp 59-65 (2021) phloridzin cox-1 cox-2 pges-1 5-lox Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.4103/2221-1691.303604 2022-12-31T00:28:41Z Objective: To evaluate the anti-inflammatory potential of aqueous extract of Pterocarpus santalinus L.f. heartwood using molecular docking and in vivo experiment. Methods: An aqueous extract of Pterocarpus santalinus heartwood was prepared using a Soxhlet apparatus. Phytocompounds in the extract were tentatively identified using high-resolution mass spectrometry. Molecular docking experiments were carried out to evaluate the binding affinity of selected compounds, phloridzin to cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), prostaglandin E synthase-1 (PGES-1) and 5-lipoxygenase (5-LOX). Anti-inflammatory potential was evaluated by carageenan induced paw edema model in rats. Results: The presence of major component phloridzin along with quercetin, parthenin, ginkgolide B, picrotoxinin, usnic acid, octopine, and epigallocatechin was detected in the extract. Molecular docking study showed that phloridzin inhibited COX-1, COX-2, PGES-1 and 5-LOX with more affinity than ibuprofen and paracetamol. Pterocarpus santalinus heartwood extract at 200 and 400 mg/kg BW showed significant reduction in carageenan-induced hind paw edema in a dose-dependent manner, but the effect was slow when compared with the standard ibuprofen (30 mg/kg p.o.). Conclusions: The study indicated that after clinical trials, the aqueous extract of Pterocarpus santalinus heartwood can be effectively used in phytotherapy to treat inflammation. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 11 2 59 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
phloridzin cox-1 cox-2 pges-1 5-lox Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
phloridzin cox-1 cox-2 pges-1 5-lox Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 C N Shanti Vasudevan Bibu John Kariyil D Athira Nair I'ma Neerakkal Screening of phytocompounds, molecular docking studies, and in vivo anti-inflammatory activity of heartwood aqueous extract of Pterocarpus santalinus L.f. |
topic_facet |
phloridzin cox-1 cox-2 pges-1 5-lox Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the anti-inflammatory potential of aqueous extract of Pterocarpus santalinus L.f. heartwood using molecular docking and in vivo experiment. Methods: An aqueous extract of Pterocarpus santalinus heartwood was prepared using a Soxhlet apparatus. Phytocompounds in the extract were tentatively identified using high-resolution mass spectrometry. Molecular docking experiments were carried out to evaluate the binding affinity of selected compounds, phloridzin to cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), prostaglandin E synthase-1 (PGES-1) and 5-lipoxygenase (5-LOX). Anti-inflammatory potential was evaluated by carageenan induced paw edema model in rats. Results: The presence of major component phloridzin along with quercetin, parthenin, ginkgolide B, picrotoxinin, usnic acid, octopine, and epigallocatechin was detected in the extract. Molecular docking study showed that phloridzin inhibited COX-1, COX-2, PGES-1 and 5-LOX with more affinity than ibuprofen and paracetamol. Pterocarpus santalinus heartwood extract at 200 and 400 mg/kg BW showed significant reduction in carageenan-induced hind paw edema in a dose-dependent manner, but the effect was slow when compared with the standard ibuprofen (30 mg/kg p.o.). Conclusions: The study indicated that after clinical trials, the aqueous extract of Pterocarpus santalinus heartwood can be effectively used in phytotherapy to treat inflammation. |
format |
Article in Journal/Newspaper |
author |
C N Shanti Vasudevan Bibu John Kariyil D Athira Nair I'ma Neerakkal |
author_facet |
C N Shanti Vasudevan Bibu John Kariyil D Athira Nair I'ma Neerakkal |
author_sort |
C N Shanti Vasudevan |
title |
Screening of phytocompounds, molecular docking studies, and in vivo anti-inflammatory activity of heartwood aqueous extract of Pterocarpus santalinus L.f. |
title_short |
Screening of phytocompounds, molecular docking studies, and in vivo anti-inflammatory activity of heartwood aqueous extract of Pterocarpus santalinus L.f. |
title_full |
Screening of phytocompounds, molecular docking studies, and in vivo anti-inflammatory activity of heartwood aqueous extract of Pterocarpus santalinus L.f. |
title_fullStr |
Screening of phytocompounds, molecular docking studies, and in vivo anti-inflammatory activity of heartwood aqueous extract of Pterocarpus santalinus L.f. |
title_full_unstemmed |
Screening of phytocompounds, molecular docking studies, and in vivo anti-inflammatory activity of heartwood aqueous extract of Pterocarpus santalinus L.f. |
title_sort |
screening of phytocompounds, molecular docking studies, and in vivo anti-inflammatory activity of heartwood aqueous extract of pterocarpus santalinus l.f. |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2021 |
url |
https://doi.org/10.4103/2221-1691.303604 https://doaj.org/article/0cdc6a6c8f844244ad5a9ca3193ff73b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 2, Pp 59-65 (2021) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=2;spage=59;epage=65;aulast=Vasudevan https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.303604 https://doaj.org/article/0cdc6a6c8f844244ad5a9ca3193ff73b |
op_doi |
https://doi.org/10.4103/2221-1691.303604 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
11 |
container_issue |
2 |
container_start_page |
59 |
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1766340045756694528 |