Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected
Detection of Toxoplasma gondii (T. gondii) DNA in blood can help to diagnose the disease in its acute phase; however, it must be considered that hemoglobin, present in blood, can inhibit polymerase activity, making impracticable the detection of DNA in samples. Mice were experimentally infected via...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/S1678-91992006000200004 https://doaj.org/article/0c9c9ddb0381463591fd76237ec344fd |
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ftdoajarticles:oai:doaj.org/article:0c9c9ddb0381463591fd76237ec344fd 2023-05-15T15:04:11+02:00 Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected H. Langoni C. B. Das Dores R. C. Silva S. B. Pezerico A. P. B. Castro A. V. Da Silva 2006-04-01T00:00:00Z https://doi.org/10.1590/S1678-91992006000200004 https://doaj.org/article/0c9c9ddb0381463591fd76237ec344fd EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200004 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992006000200004 1678-9199 https://doaj.org/article/0c9c9ddb0381463591fd76237ec344fd Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 12, Iss 2, Pp 202-214 (2006) toxoplasmosis PCR serum experimental infection mice Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2006 ftdoajarticles https://doi.org/10.1590/S1678-91992006000200004 2022-12-31T06:24:42Z Detection of Toxoplasma gondii (T. gondii) DNA in blood can help to diagnose the disease in its acute phase; however, it must be considered that hemoglobin, present in blood, can inhibit polymerase activity, making impracticable the detection of DNA in samples. Mice were experimentally infected via oral route with ME49 and BTU2 strains cysts and RH strain tachyzoites; polymerase chain reaction was used to detect T. gondii DNA in mice sera 18, 24, 48, 96, and 192 hours post infection (PI). Toxoplama gondii DNA was detected in only one animal infected with BTU2 strain, genotype III (isolated from a dog with neurological signs) 18 hours PI. The agent's DNA was not detected in any sample of the other experimental groups. New studies must be carried out to verify the technique sensitivity in researches on this agent's genetic material using sera samples of acute-phase toxoplasmosis patients, especially in cases of immunosuppression. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 12 2 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
toxoplasmosis PCR serum experimental infection mice Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
toxoplasmosis PCR serum experimental infection mice Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 H. Langoni C. B. Das Dores R. C. Silva S. B. Pezerico A. P. B. Castro A. V. Da Silva Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected |
topic_facet |
toxoplasmosis PCR serum experimental infection mice Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Detection of Toxoplasma gondii (T. gondii) DNA in blood can help to diagnose the disease in its acute phase; however, it must be considered that hemoglobin, present in blood, can inhibit polymerase activity, making impracticable the detection of DNA in samples. Mice were experimentally infected via oral route with ME49 and BTU2 strains cysts and RH strain tachyzoites; polymerase chain reaction was used to detect T. gondii DNA in mice sera 18, 24, 48, 96, and 192 hours post infection (PI). Toxoplama gondii DNA was detected in only one animal infected with BTU2 strain, genotype III (isolated from a dog with neurological signs) 18 hours PI. The agent's DNA was not detected in any sample of the other experimental groups. New studies must be carried out to verify the technique sensitivity in researches on this agent's genetic material using sera samples of acute-phase toxoplasmosis patients, especially in cases of immunosuppression. |
format |
Article in Journal/Newspaper |
author |
H. Langoni C. B. Das Dores R. C. Silva S. B. Pezerico A. P. B. Castro A. V. Da Silva |
author_facet |
H. Langoni C. B. Das Dores R. C. Silva S. B. Pezerico A. P. B. Castro A. V. Da Silva |
author_sort |
H. Langoni |
title |
Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected |
title_short |
Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected |
title_full |
Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected |
title_fullStr |
Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected |
title_full_unstemmed |
Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected |
title_sort |
detection of toxoplasma gondii dna in sera samples of mice experimentally infected |
publisher |
SciELO |
publishDate |
2006 |
url |
https://doi.org/10.1590/S1678-91992006000200004 https://doaj.org/article/0c9c9ddb0381463591fd76237ec344fd |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 12, Iss 2, Pp 202-214 (2006) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200004 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992006000200004 1678-9199 https://doaj.org/article/0c9c9ddb0381463591fd76237ec344fd |
op_doi |
https://doi.org/10.1590/S1678-91992006000200004 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
12 |
container_issue |
2 |
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1766335991644160000 |