Transcriptomic profiling of gametogenesis in triploid Pacific Oysters Crassostrea gigas: towards an understanding of partial sterility associated with triploidy.
Triploidy can occur in many animal species but is often lethal. Among invertebrates, amphibians and fishes, triploids are viable although often sterile or infertile. Most triploids of the Pacific oyster Crassostrea gigas are almost sterile (named "3nβ") yet a low but significant proportion...
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ftdoajarticles:oai:doaj.org/article:0c657a4acbdc4935b171de609691c37e 2023-05-15T15:58:12+02:00 Transcriptomic profiling of gametogenesis in triploid Pacific Oysters Crassostrea gigas: towards an understanding of partial sterility associated with triploidy. Nolwenn M Dheilly Aude Jouaux Pierre Boudry Pascal Favrel Christophe Lelong 2014-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0112094 https://doaj.org/article/0c657a4acbdc4935b171de609691c37e EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4222980?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0112094 https://doaj.org/article/0c657a4acbdc4935b171de609691c37e PLoS ONE, Vol 9, Iss 11, p e112094 (2014) Medicine R Science Q article 2014 ftdoajarticles https://doi.org/10.1371/journal.pone.0112094 2022-12-31T03:45:15Z Triploidy can occur in many animal species but is often lethal. Among invertebrates, amphibians and fishes, triploids are viable although often sterile or infertile. Most triploids of the Pacific oyster Crassostrea gigas are almost sterile (named "3nβ") yet a low but significant proportion show an advanced gametogenesis (named "3nα"). These oysters thus constitute an interesting model to study the effect of triploidy on germ cell development. We used microarrays to compare the gonad transcriptomes of diploid 2n and the abovementioned triploid 3nβ and 3nα male and female oysters throughout gametogenesis.All triploids displayed an upregulation of genes related to DNA repair and apoptosis and a downregulation of genes associated with cell division. The comparison of 3nα and 3nβ transcriptomes with 2n revealed the likely involvement of a cell cycle checkpoint during mitosis in the successful but delayed development of gonads in 3nα individuals. In contrast, a disruption of sex differentiation mechanisms may explain the sterility of 3nβ individuals with 3nβ females expressing male-specific genes and 3nβ males expressing female-specific genes.The disruption of sex differentiation and mitosis may be responsible for the impaired gametogenesis of triploid Pacific oysters. The function of the numerous candidate genes identified in our study should now be studied in detail in order to elucidate their role in sex determination, mitosis/meiosis control, pachytene cell cycle checkpoint, and the control of DNA repair/apoptosis. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific PLoS ONE 9 11 e112094 |
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Medicine R Science Q |
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Medicine R Science Q Nolwenn M Dheilly Aude Jouaux Pierre Boudry Pascal Favrel Christophe Lelong Transcriptomic profiling of gametogenesis in triploid Pacific Oysters Crassostrea gigas: towards an understanding of partial sterility associated with triploidy. |
topic_facet |
Medicine R Science Q |
description |
Triploidy can occur in many animal species but is often lethal. Among invertebrates, amphibians and fishes, triploids are viable although often sterile or infertile. Most triploids of the Pacific oyster Crassostrea gigas are almost sterile (named "3nβ") yet a low but significant proportion show an advanced gametogenesis (named "3nα"). These oysters thus constitute an interesting model to study the effect of triploidy on germ cell development. We used microarrays to compare the gonad transcriptomes of diploid 2n and the abovementioned triploid 3nβ and 3nα male and female oysters throughout gametogenesis.All triploids displayed an upregulation of genes related to DNA repair and apoptosis and a downregulation of genes associated with cell division. The comparison of 3nα and 3nβ transcriptomes with 2n revealed the likely involvement of a cell cycle checkpoint during mitosis in the successful but delayed development of gonads in 3nα individuals. In contrast, a disruption of sex differentiation mechanisms may explain the sterility of 3nβ individuals with 3nβ females expressing male-specific genes and 3nβ males expressing female-specific genes.The disruption of sex differentiation and mitosis may be responsible for the impaired gametogenesis of triploid Pacific oysters. The function of the numerous candidate genes identified in our study should now be studied in detail in order to elucidate their role in sex determination, mitosis/meiosis control, pachytene cell cycle checkpoint, and the control of DNA repair/apoptosis. |
format |
Article in Journal/Newspaper |
author |
Nolwenn M Dheilly Aude Jouaux Pierre Boudry Pascal Favrel Christophe Lelong |
author_facet |
Nolwenn M Dheilly Aude Jouaux Pierre Boudry Pascal Favrel Christophe Lelong |
author_sort |
Nolwenn M Dheilly |
title |
Transcriptomic profiling of gametogenesis in triploid Pacific Oysters Crassostrea gigas: towards an understanding of partial sterility associated with triploidy. |
title_short |
Transcriptomic profiling of gametogenesis in triploid Pacific Oysters Crassostrea gigas: towards an understanding of partial sterility associated with triploidy. |
title_full |
Transcriptomic profiling of gametogenesis in triploid Pacific Oysters Crassostrea gigas: towards an understanding of partial sterility associated with triploidy. |
title_fullStr |
Transcriptomic profiling of gametogenesis in triploid Pacific Oysters Crassostrea gigas: towards an understanding of partial sterility associated with triploidy. |
title_full_unstemmed |
Transcriptomic profiling of gametogenesis in triploid Pacific Oysters Crassostrea gigas: towards an understanding of partial sterility associated with triploidy. |
title_sort |
transcriptomic profiling of gametogenesis in triploid pacific oysters crassostrea gigas: towards an understanding of partial sterility associated with triploidy. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pone.0112094 https://doaj.org/article/0c657a4acbdc4935b171de609691c37e |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
PLoS ONE, Vol 9, Iss 11, p e112094 (2014) |
op_relation |
http://europepmc.org/articles/PMC4222980?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0112094 https://doaj.org/article/0c657a4acbdc4935b171de609691c37e |
op_doi |
https://doi.org/10.1371/journal.pone.0112094 |
container_title |
PLoS ONE |
container_volume |
9 |
container_issue |
11 |
container_start_page |
e112094 |
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