Restriction enzyme analysis of the human cytomegalovirus genome in specimens collected from immunodeficient patients in Belém, State of Pará, Brazil
INTRODUCTION: Human cytomegalovirus is an opportunistic betaherpesvirus that causes persistent and serious infections in immunodeficient patients. Recurrent infections occur due to the presence of the virus in a latent state in some cell types. It is possible to examine the virus using molecular met...
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ftdoajarticles:oai:doaj.org/article:0c397571a1af4324ad4606fbb0e3f9be 2023-05-15T15:11:44+02:00 Restriction enzyme analysis of the human cytomegalovirus genome in specimens collected from immunodeficient patients in Belém, State of Pará, Brazil Dorotéa Lobato da Silva Renato Lopes Fernandes de Medeiros Marluce Matos de Moraes Fernanda Sagicado Espírito Santo 2011-10-01T00:00:00Z https://doi.org/10.1590/s0037-86822011005000052 https://doaj.org/article/0c397571a1af4324ad4606fbb0e3f9be EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000500004&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/s0037-86822011005000052 https://doaj.org/article/0c397571a1af4324ad4606fbb0e3f9be Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 5, Pp 551-554 (2011) Citomegalovírus Imunodeficiente Diversidade genotípica DNA viral RFLP Arctic medicine. Tropical medicine RC955-962 article 2011 ftdoajarticles https://doi.org/10.1590/s0037-86822011005000052 2022-12-30T21:26:57Z INTRODUCTION: Human cytomegalovirus is an opportunistic betaherpesvirus that causes persistent and serious infections in immunodeficient patients. Recurrent infections occur due to the presence of the virus in a latent state in some cell types. It is possible to examine the virus using molecular methods to aid in the immunological diagnosis and to generate a molecular viral profile in immunodeficient patients. The objective of this study was to characterize cytomegalovirus genotypes and to generate the epidemiological and molecular viral profile in immunodeficient patients. METHODS: A total of 105 samples were collected from immunodeficient patients from the City of Belém, including newborns, hemodialysis patients, transplant recipients and HIV+ patients. An IgG and IgM antibody study was completed using ELISA, and enzymatic analysis by restriction fragment length polymorphism (RFLP) was performed to characterize viral genotypes. RESULTS: It was observed that 100% of the patients had IgG antibodies, 87% of which were IgG+/IgM-, consistent with a prior infection profile, 13% were IgG+/IgM+, suggestive of recent infection. The newborn group had the highest frequency (27%) of the IgG+/IgM+ profile. By RFLP analysis, only one genotype was observed, gB2, which corresponded to the standard AD169 strain. CONCLUSIONS: The presence of IgM antibodies in new borns indicates that HCMV continues to be an important cause of congenital infection. The low observed genotypic diversity could be attributed to the small sample size because newborns were excluded from the RFLP analysis. This study will be continued including samples from newborns to extend the knowledge of the general and molecular epidemiology of HCMV in immunodeficient patients. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 44 5 551 554 |
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Citomegalovírus Imunodeficiente Diversidade genotípica DNA viral RFLP Arctic medicine. Tropical medicine RC955-962 |
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Citomegalovírus Imunodeficiente Diversidade genotípica DNA viral RFLP Arctic medicine. Tropical medicine RC955-962 Dorotéa Lobato da Silva Renato Lopes Fernandes de Medeiros Marluce Matos de Moraes Fernanda Sagicado Espírito Santo Restriction enzyme analysis of the human cytomegalovirus genome in specimens collected from immunodeficient patients in Belém, State of Pará, Brazil |
topic_facet |
Citomegalovírus Imunodeficiente Diversidade genotípica DNA viral RFLP Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: Human cytomegalovirus is an opportunistic betaherpesvirus that causes persistent and serious infections in immunodeficient patients. Recurrent infections occur due to the presence of the virus in a latent state in some cell types. It is possible to examine the virus using molecular methods to aid in the immunological diagnosis and to generate a molecular viral profile in immunodeficient patients. The objective of this study was to characterize cytomegalovirus genotypes and to generate the epidemiological and molecular viral profile in immunodeficient patients. METHODS: A total of 105 samples were collected from immunodeficient patients from the City of Belém, including newborns, hemodialysis patients, transplant recipients and HIV+ patients. An IgG and IgM antibody study was completed using ELISA, and enzymatic analysis by restriction fragment length polymorphism (RFLP) was performed to characterize viral genotypes. RESULTS: It was observed that 100% of the patients had IgG antibodies, 87% of which were IgG+/IgM-, consistent with a prior infection profile, 13% were IgG+/IgM+, suggestive of recent infection. The newborn group had the highest frequency (27%) of the IgG+/IgM+ profile. By RFLP analysis, only one genotype was observed, gB2, which corresponded to the standard AD169 strain. CONCLUSIONS: The presence of IgM antibodies in new borns indicates that HCMV continues to be an important cause of congenital infection. The low observed genotypic diversity could be attributed to the small sample size because newborns were excluded from the RFLP analysis. This study will be continued including samples from newborns to extend the knowledge of the general and molecular epidemiology of HCMV in immunodeficient patients. |
format |
Article in Journal/Newspaper |
author |
Dorotéa Lobato da Silva Renato Lopes Fernandes de Medeiros Marluce Matos de Moraes Fernanda Sagicado Espírito Santo |
author_facet |
Dorotéa Lobato da Silva Renato Lopes Fernandes de Medeiros Marluce Matos de Moraes Fernanda Sagicado Espírito Santo |
author_sort |
Dorotéa Lobato da Silva |
title |
Restriction enzyme analysis of the human cytomegalovirus genome in specimens collected from immunodeficient patients in Belém, State of Pará, Brazil |
title_short |
Restriction enzyme analysis of the human cytomegalovirus genome in specimens collected from immunodeficient patients in Belém, State of Pará, Brazil |
title_full |
Restriction enzyme analysis of the human cytomegalovirus genome in specimens collected from immunodeficient patients in Belém, State of Pará, Brazil |
title_fullStr |
Restriction enzyme analysis of the human cytomegalovirus genome in specimens collected from immunodeficient patients in Belém, State of Pará, Brazil |
title_full_unstemmed |
Restriction enzyme analysis of the human cytomegalovirus genome in specimens collected from immunodeficient patients in Belém, State of Pará, Brazil |
title_sort |
restriction enzyme analysis of the human cytomegalovirus genome in specimens collected from immunodeficient patients in belém, state of pará, brazil |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2011 |
url |
https://doi.org/10.1590/s0037-86822011005000052 https://doaj.org/article/0c397571a1af4324ad4606fbb0e3f9be |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 5, Pp 551-554 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000500004&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/s0037-86822011005000052 https://doaj.org/article/0c397571a1af4324ad4606fbb0e3f9be |
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https://doi.org/10.1590/s0037-86822011005000052 |
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44 |
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