Detection of proteases from Sporosarcina aquimarina and Algoriphagus antarcticus isolated from Antarctic soil
Two psychrophilic bacterial samples were isolated from King George Island soil, in Antarctica. The phylogenetic analysis based on the 16S rRNA (rrs) gene led to the correlation with the closest related isolates as Sporosarcina aquimarina (99%) and Algoriphagus antarcticus(99%), with query coverage o...
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ftdoajarticles:oai:doaj.org/article:0c2a0bb3695f425e858ac36c03bf4ed6 2023-05-15T14:06:38+02:00 Detection of proteases from Sporosarcina aquimarina and Algoriphagus antarcticus isolated from Antarctic soil Anderson F. Santos Fabiano Pires Hugo E. Jesus André L.S. Santos Raquel Peixoto Alexandre S. Rosado Claudia M. D'Avila-Levy Marta H. Branquinha 2015-03-01T00:00:00Z https://doi.org/10.1590/0001-3765201520130519 https://doaj.org/article/0c2a0bb3695f425e858ac36c03bf4ed6 EN eng Academia Brasileira de Ciências http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100109&lng=en&tlng=en https://doaj.org/toc/1678-2690 1678-2690 doi:10.1590/0001-3765201520130519 https://doaj.org/article/0c2a0bb3695f425e858ac36c03bf4ed6 Anais da Academia Brasileira de Ciências, Vol 87, Iss 1, Pp 109-119 (2015) Algoriphagus antarcticus Antártica Sporosarcina aquimarina Protease Science Q article 2015 ftdoajarticles https://doi.org/10.1590/0001-3765201520130519 2022-12-31T13:40:54Z Two psychrophilic bacterial samples were isolated from King George Island soil, in Antarctica. The phylogenetic analysis based on the 16S rRNA (rrs) gene led to the correlation with the closest related isolates as Sporosarcina aquimarina (99%) and Algoriphagus antarcticus(99%), with query coverage of 99% and 98%, respectively.The spent culture media from both isolates displayed proteolytic activities detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis containing gelatin as protein substrate. Under the employed conditions, S. aquimarina showed a 55 kDa protease with the best activity detected at pH 7.0 and at 27°C. A. antarcticusalso showed a single extracellular protease, however its molecular mass was around 90kDa and its best activity was detected at pH 9.0 and at 37°C. The proteases from both isolates were inhibited by 1,10-phenanthroline and EDTA, two metalloprotease inhibitors. This is the first record of protease detection in both species, and our results may contribute to broaden the basic knowledge of proteases from the Antarctica environment and may help prospecting future biotechnological applications of these enzymes. Article in Journal/Newspaper Antarc* Antarctic Antarctica antarcticus Antártica King George Island Directory of Open Access Journals: DOAJ Articles Antarctic King George Island Anais da Academia Brasileira de Ciências 87 1 109 119 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Algoriphagus antarcticus Antártica Sporosarcina aquimarina Protease Science Q |
spellingShingle |
Algoriphagus antarcticus Antártica Sporosarcina aquimarina Protease Science Q Anderson F. Santos Fabiano Pires Hugo E. Jesus André L.S. Santos Raquel Peixoto Alexandre S. Rosado Claudia M. D'Avila-Levy Marta H. Branquinha Detection of proteases from Sporosarcina aquimarina and Algoriphagus antarcticus isolated from Antarctic soil |
topic_facet |
Algoriphagus antarcticus Antártica Sporosarcina aquimarina Protease Science Q |
description |
Two psychrophilic bacterial samples were isolated from King George Island soil, in Antarctica. The phylogenetic analysis based on the 16S rRNA (rrs) gene led to the correlation with the closest related isolates as Sporosarcina aquimarina (99%) and Algoriphagus antarcticus(99%), with query coverage of 99% and 98%, respectively.The spent culture media from both isolates displayed proteolytic activities detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis containing gelatin as protein substrate. Under the employed conditions, S. aquimarina showed a 55 kDa protease with the best activity detected at pH 7.0 and at 27°C. A. antarcticusalso showed a single extracellular protease, however its molecular mass was around 90kDa and its best activity was detected at pH 9.0 and at 37°C. The proteases from both isolates were inhibited by 1,10-phenanthroline and EDTA, two metalloprotease inhibitors. This is the first record of protease detection in both species, and our results may contribute to broaden the basic knowledge of proteases from the Antarctica environment and may help prospecting future biotechnological applications of these enzymes. |
format |
Article in Journal/Newspaper |
author |
Anderson F. Santos Fabiano Pires Hugo E. Jesus André L.S. Santos Raquel Peixoto Alexandre S. Rosado Claudia M. D'Avila-Levy Marta H. Branquinha |
author_facet |
Anderson F. Santos Fabiano Pires Hugo E. Jesus André L.S. Santos Raquel Peixoto Alexandre S. Rosado Claudia M. D'Avila-Levy Marta H. Branquinha |
author_sort |
Anderson F. Santos |
title |
Detection of proteases from Sporosarcina aquimarina and Algoriphagus antarcticus isolated from Antarctic soil |
title_short |
Detection of proteases from Sporosarcina aquimarina and Algoriphagus antarcticus isolated from Antarctic soil |
title_full |
Detection of proteases from Sporosarcina aquimarina and Algoriphagus antarcticus isolated from Antarctic soil |
title_fullStr |
Detection of proteases from Sporosarcina aquimarina and Algoriphagus antarcticus isolated from Antarctic soil |
title_full_unstemmed |
Detection of proteases from Sporosarcina aquimarina and Algoriphagus antarcticus isolated from Antarctic soil |
title_sort |
detection of proteases from sporosarcina aquimarina and algoriphagus antarcticus isolated from antarctic soil |
publisher |
Academia Brasileira de Ciências |
publishDate |
2015 |
url |
https://doi.org/10.1590/0001-3765201520130519 https://doaj.org/article/0c2a0bb3695f425e858ac36c03bf4ed6 |
geographic |
Antarctic King George Island |
geographic_facet |
Antarctic King George Island |
genre |
Antarc* Antarctic Antarctica antarcticus Antártica King George Island |
genre_facet |
Antarc* Antarctic Antarctica antarcticus Antártica King George Island |
op_source |
Anais da Academia Brasileira de Ciências, Vol 87, Iss 1, Pp 109-119 (2015) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100109&lng=en&tlng=en https://doaj.org/toc/1678-2690 1678-2690 doi:10.1590/0001-3765201520130519 https://doaj.org/article/0c2a0bb3695f425e858ac36c03bf4ed6 |
op_doi |
https://doi.org/10.1590/0001-3765201520130519 |
container_title |
Anais da Academia Brasileira de Ciências |
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87 |
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1 |
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109 |
op_container_end_page |
119 |
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1766278614731456512 |