Screening of a library of traditional Chinese medicines to identify anti-malarial compounds and extracts
Abstract Background Malaria is a major infectious disease in the world. In 2015, approximately 212 million people were infected and 429,000 people were killed by this disease. Plasmodium falciparum, which causes falciparum malaria, is becoming resistant to artemisinin (ART) in Southeast Asia; theref...
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ftdoajarticles:oai:doaj.org/article:0c28a0b7f076422982fda379c5acb7f8 2023-05-15T15:16:12+02:00 Screening of a library of traditional Chinese medicines to identify anti-malarial compounds and extracts Motohiro Nonaka Yuho Murata Ryo Takano Yongmei Han Md. Hazzaz Bin Kabir Kentaro Kato 2018-06-01T00:00:00Z https://doi.org/10.1186/s12936-018-2392-4 https://doaj.org/article/0c28a0b7f076422982fda379c5acb7f8 EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2392-4 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2392-4 1475-2875 https://doaj.org/article/0c28a0b7f076422982fda379c5acb7f8 Malaria Journal, Vol 17, Iss 1, Pp 1-10 (2018) Antimalarial drugs Drug screening Traditional Chinese medicine Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2392-4 2022-12-31T09:38:17Z Abstract Background Malaria is a major infectious disease in the world. In 2015, approximately 212 million people were infected and 429,000 people were killed by this disease. Plasmodium falciparum, which causes falciparum malaria, is becoming resistant to artemisinin (ART) in Southeast Asia; therefore, new anti-malarial drugs are urgently needed. Some excellent anti-malarial drugs, such as quinine or ART, were originally obtained from natural plants. Hence, the authors screened a natural product library comprising traditional Chinese medicines (TCMs) to identify compounds/extracts with anti-malarial effects. Methods The authors performed three assays: a malaria growth inhibition assay (GIA), a cytotoxicity assay, and a malaria stage-specific GIA. The malaria GIA revealed the anti-malarial ability and half-maximal inhibitory concentrations (IC50) of the natural products, whereas the malaria stage-specific GIA revealed the point in the malaria life cycle where the products exerted their anti-malarial effects. The toxicity of the products to the host cells was evaluated with the cytotoxicity assay. Results Four natural compounds (berberine chloride, coptisine chloride, palmatine chloride, and dehydrocorydaline nitrate) showed strong anti-malarial effects (IC50 < 50 nM), and low cytotoxicity (cell viability > 90%) using P. falciparum 3D7 strain. Two natural extracts (Phellodendri cortex and Coptidis rhizoma) also showed strong antiplasmodial effects (IC50 < 1 µg/ml), and low cytotoxicity (cell viability > 80%). These natural products also demonstrated anti-malarial capability during the trophozoite and schizont stages of the malaria life cycle. Conclusions The authors identified four compounds (berberine chloride, coptisine chloride, palmatine chloride, and dehydrocorydaline nitrate) and two extracts (Phellodendri cortex and Coptidis rhizoma) with anti-malarial activity, neither of which had previously been described. The IC50 values of the compounds were comparable to that of chloroquine and better ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 17 1 |
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Antimalarial drugs Drug screening Traditional Chinese medicine Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Antimalarial drugs Drug screening Traditional Chinese medicine Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Motohiro Nonaka Yuho Murata Ryo Takano Yongmei Han Md. Hazzaz Bin Kabir Kentaro Kato Screening of a library of traditional Chinese medicines to identify anti-malarial compounds and extracts |
topic_facet |
Antimalarial drugs Drug screening Traditional Chinese medicine Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria is a major infectious disease in the world. In 2015, approximately 212 million people were infected and 429,000 people were killed by this disease. Plasmodium falciparum, which causes falciparum malaria, is becoming resistant to artemisinin (ART) in Southeast Asia; therefore, new anti-malarial drugs are urgently needed. Some excellent anti-malarial drugs, such as quinine or ART, were originally obtained from natural plants. Hence, the authors screened a natural product library comprising traditional Chinese medicines (TCMs) to identify compounds/extracts with anti-malarial effects. Methods The authors performed three assays: a malaria growth inhibition assay (GIA), a cytotoxicity assay, and a malaria stage-specific GIA. The malaria GIA revealed the anti-malarial ability and half-maximal inhibitory concentrations (IC50) of the natural products, whereas the malaria stage-specific GIA revealed the point in the malaria life cycle where the products exerted their anti-malarial effects. The toxicity of the products to the host cells was evaluated with the cytotoxicity assay. Results Four natural compounds (berberine chloride, coptisine chloride, palmatine chloride, and dehydrocorydaline nitrate) showed strong anti-malarial effects (IC50 < 50 nM), and low cytotoxicity (cell viability > 90%) using P. falciparum 3D7 strain. Two natural extracts (Phellodendri cortex and Coptidis rhizoma) also showed strong antiplasmodial effects (IC50 < 1 µg/ml), and low cytotoxicity (cell viability > 80%). These natural products also demonstrated anti-malarial capability during the trophozoite and schizont stages of the malaria life cycle. Conclusions The authors identified four compounds (berberine chloride, coptisine chloride, palmatine chloride, and dehydrocorydaline nitrate) and two extracts (Phellodendri cortex and Coptidis rhizoma) with anti-malarial activity, neither of which had previously been described. The IC50 values of the compounds were comparable to that of chloroquine and better ... |
format |
Article in Journal/Newspaper |
author |
Motohiro Nonaka Yuho Murata Ryo Takano Yongmei Han Md. Hazzaz Bin Kabir Kentaro Kato |
author_facet |
Motohiro Nonaka Yuho Murata Ryo Takano Yongmei Han Md. Hazzaz Bin Kabir Kentaro Kato |
author_sort |
Motohiro Nonaka |
title |
Screening of a library of traditional Chinese medicines to identify anti-malarial compounds and extracts |
title_short |
Screening of a library of traditional Chinese medicines to identify anti-malarial compounds and extracts |
title_full |
Screening of a library of traditional Chinese medicines to identify anti-malarial compounds and extracts |
title_fullStr |
Screening of a library of traditional Chinese medicines to identify anti-malarial compounds and extracts |
title_full_unstemmed |
Screening of a library of traditional Chinese medicines to identify anti-malarial compounds and extracts |
title_sort |
screening of a library of traditional chinese medicines to identify anti-malarial compounds and extracts |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2392-4 https://doaj.org/article/0c28a0b7f076422982fda379c5acb7f8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-10 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2392-4 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2392-4 1475-2875 https://doaj.org/article/0c28a0b7f076422982fda379c5acb7f8 |
op_doi |
https://doi.org/10.1186/s12936-018-2392-4 |
container_title |
Malaria Journal |
container_volume |
17 |
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1 |
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1766346494571446272 |