Effect of crocin carotenoid on BDNF and CREB gene expression in brain ventral tegmental area of morphine treated rats
Objective: To investigate the effect of crocin carotenoid on BNDF and CREB gene expression in the brain ventral tegmental area (VTA) and the serum level of BDNF in morphine-treated rats compared to control. Methods: In this study, 40 male Wistar rats (200-250 g) were used in 5 experimental groups: 1...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:0c069afbc9b0481a9a652c4020602087 2023-05-15T15:11:47+02:00 Effect of crocin carotenoid on BDNF and CREB gene expression in brain ventral tegmental area of morphine treated rats Marzieh Rezai Mehdi Mahmoodi Ayat Kaeidi Mojgan Noroozi Karimabad Alireza Khoshdel Mohammad Reza Hajizadeh 2018-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.239426 https://doaj.org/article/0c069afbc9b0481a9a652c4020602087 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=8;spage=387;epage=393;aulast=Rezai https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.239426 https://doaj.org/article/0c069afbc9b0481a9a652c4020602087 Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 8, Pp 387-393 (2018) morphine bdnf creb crocin ventral tegmental area rat Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.4103/2221-1691.239426 2022-12-31T09:50:15Z Objective: To investigate the effect of crocin carotenoid on BNDF and CREB gene expression in the brain ventral tegmental area (VTA) and the serum level of BDNF in morphine-treated rats compared to control. Methods: In this study, 40 male Wistar rats (200-250 g) were used in 5 experimental groups: 1) non morphine treat rats (control); 2) non morphine-treated rats with 25 mg/kg crocin carotenoid (i.p., for 21 d); 3) morphine treated rats (10 mg/kg twice a day, s.c., 21 d); 4 and 5) morphine-treated rats with 12.5 and 25 mg/kg crocin carotenoid, respectively. By the end of research, BDNF and CREB expression was determined by real-time-PCR method. ELISA analysis was also applied for assessing the serum BDNF level. Results: The data indicated that morphine treatment could cause a significant decrease in BDNF and CREB gene expression (P<0.01 and P<0.001, respectively) in brain VTA as well as serum level of BDNF (P<0.01) in comparison to control group. Treatment with 25 mg/kg crocin carotenoid caused a significant enhancement in BDNF and CREF gene expression (P<0.01 and P<0.05, respectively) and serum level of BDNF (P<0.01) in morphine-treated rats in comparison to morphine-treated group. Conclusions: Regarding to obtained results, crocin carotenoid can inhibit unfavorable effects of morphine on the neural system to some extent through enhancing BDNF and CREB gene expression in brain VTA and serum level of BDNF. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 8 8 387 |
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morphine bdnf creb crocin ventral tegmental area rat Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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morphine bdnf creb crocin ventral tegmental area rat Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Marzieh Rezai Mehdi Mahmoodi Ayat Kaeidi Mojgan Noroozi Karimabad Alireza Khoshdel Mohammad Reza Hajizadeh Effect of crocin carotenoid on BDNF and CREB gene expression in brain ventral tegmental area of morphine treated rats |
topic_facet |
morphine bdnf creb crocin ventral tegmental area rat Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the effect of crocin carotenoid on BNDF and CREB gene expression in the brain ventral tegmental area (VTA) and the serum level of BDNF in morphine-treated rats compared to control. Methods: In this study, 40 male Wistar rats (200-250 g) were used in 5 experimental groups: 1) non morphine treat rats (control); 2) non morphine-treated rats with 25 mg/kg crocin carotenoid (i.p., for 21 d); 3) morphine treated rats (10 mg/kg twice a day, s.c., 21 d); 4 and 5) morphine-treated rats with 12.5 and 25 mg/kg crocin carotenoid, respectively. By the end of research, BDNF and CREB expression was determined by real-time-PCR method. ELISA analysis was also applied for assessing the serum BDNF level. Results: The data indicated that morphine treatment could cause a significant decrease in BDNF and CREB gene expression (P<0.01 and P<0.001, respectively) in brain VTA as well as serum level of BDNF (P<0.01) in comparison to control group. Treatment with 25 mg/kg crocin carotenoid caused a significant enhancement in BDNF and CREF gene expression (P<0.01 and P<0.05, respectively) and serum level of BDNF (P<0.01) in morphine-treated rats in comparison to morphine-treated group. Conclusions: Regarding to obtained results, crocin carotenoid can inhibit unfavorable effects of morphine on the neural system to some extent through enhancing BDNF and CREB gene expression in brain VTA and serum level of BDNF. |
format |
Article in Journal/Newspaper |
author |
Marzieh Rezai Mehdi Mahmoodi Ayat Kaeidi Mojgan Noroozi Karimabad Alireza Khoshdel Mohammad Reza Hajizadeh |
author_facet |
Marzieh Rezai Mehdi Mahmoodi Ayat Kaeidi Mojgan Noroozi Karimabad Alireza Khoshdel Mohammad Reza Hajizadeh |
author_sort |
Marzieh Rezai |
title |
Effect of crocin carotenoid on BDNF and CREB gene expression in brain ventral tegmental area of morphine treated rats |
title_short |
Effect of crocin carotenoid on BDNF and CREB gene expression in brain ventral tegmental area of morphine treated rats |
title_full |
Effect of crocin carotenoid on BDNF and CREB gene expression in brain ventral tegmental area of morphine treated rats |
title_fullStr |
Effect of crocin carotenoid on BDNF and CREB gene expression in brain ventral tegmental area of morphine treated rats |
title_full_unstemmed |
Effect of crocin carotenoid on BDNF and CREB gene expression in brain ventral tegmental area of morphine treated rats |
title_sort |
effect of crocin carotenoid on bdnf and creb gene expression in brain ventral tegmental area of morphine treated rats |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/2221-1691.239426 https://doaj.org/article/0c069afbc9b0481a9a652c4020602087 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 8, Pp 387-393 (2018) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=8;spage=387;epage=393;aulast=Rezai https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.239426 https://doaj.org/article/0c069afbc9b0481a9a652c4020602087 |
op_doi |
https://doi.org/10.4103/2221-1691.239426 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
8 |
container_issue |
8 |
container_start_page |
387 |
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1766342577190076416 |